HYPOGLYCEMIC EFFECTS OF BARLERIA NOCTIFLORA FRACTIONS ON HIGH FAT FED WITH LOW DOSE STREPTOZOTOCIN INDUCED TYPE-2 DIABETES IN RATS
Keywords:
Antidiabetic, Barleria noctiflora, Streptozotocin, Type-2 diabetesAbstract
Objective: To investigate in vitro and in vivo antidiabetic activity of Barleria noctiflora (B. noctiflora) fractions on high-fat diet (HFD) with low dose Streptozotocin (STZ) induced type-2 diabetes in rats.
Methods: B. noctiflora were successively extracted and then fractionated by Ethyl acetate and n. butanol. The in vitro antidiabetic activity from α-amylase and α-glucosidase was used to evaluate the potential activity of the fractions. The in vivo antidiabetic activity is evaluated against HFD/STZ induced type-2 diabetic in rats at a dose of 100 mg/kg, 200 mg/kg and 400 mg/kg, p. o for 28days.
Results: Ethyl acetate fraction of B. noctiflora (EAFBN) showed the highest antidiabetic activity and IC50 values of α-amylase inhibition (114.7±0.15) and α-glucosidase inhibition (104.93±0.28) than other fraction. In HFD/STZ (40 mg/kg) diabetic rats the EAFBN showed a dose dependent significant hypoglycemic property from body weight, blood glucose, serum lipids, serum cholesterol, serum triglycerides, urea, creatinine, hepatic enzymes and liver glycogen levels. The EAFBN significantly (P<0.01) increase the level of serum insulin. Histologically, focal necrosis was observed in the diabetic rat pancreas; however, was less obvious in treated groups.
Conclusion: The EAFBN is a potent hypoglycemic agent and beneficial in reducing the elevated blood glucose level, improve the lipid profile and insulin level and histopathological changes in the pancreas of HFD/STZâ€induced nonâ€genetic rat model of type-2 diabetes mellitus.
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