EVALUATION OF THE COMBINED ANTIBACTERIAL ACTIVITY OF KAEMPFERIA PANDURATA RHIZOME AND SENNA ALATA LEAVES AGAINST METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS

Authors

  • Pratiwi Wikaningtyas School of pharmacy, Bandung Institute of technology
  • Elin Yulinah Sukandar

Abstract

Objectives: The present study was performed to show the combination in vitro activities of ethanolic extract and ethyl acetate fraction of Kaempferia
pandurata and Senna alata against methicillin-resistant Staphylococcus aureus (MRSA).
Methods: The antibacterial activities were calculated based on minimum inhibitory concentration (MIC) using microdilution method and minimum
bactericidal concentration. The antimicrobial interaction between plant extract with antibiotic was performed using the paper disc and checkerboard
method.
Results: The similar MIC value was displayed by ethyl acetate fraction of K. pandurata and S. alata (128 µg/mL) while the ethanolic extract of
K. pandurata showed lower than S. alata, 512 µg/mL and 256 µg/mL, respectively. The combination between the ethanolic extract of K. pandurata
with S. alata showed synergism in all MIC tested and its fraction combination showed synergism in selected MIC value.
Conclusions: The observed antimicrobial efficacy and synergistic interactions indicate the beneficial aspects for the MRSA treatment.
Keywords: Antimicrobial, Interaction, Kaempferia pandurata, Senna alata, Minimum inhibition concentration, Checkerboard, Synergism.

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References

REFERENCES

Bukharie HA. A review of community-acquired methicillin-resistant

Staphylococcus aureus for primary care physicians. J Family

Community Med 2010;17(3):117-20.

Batabyal B, Kundu GK, Biswas S. Methicillin-resistant Staphylococcus

aureus: A brief review. Int Res J Biol Sci 2012;1(7):65-71.

Ofokansi KC, Attama AA, Uzor PF, Ovri MO. Evaluation of the

combined antimicrobial activity of the leaf extract of Phyllanthus

muellerianus with ciprofloxacin. J Pharm Technol Drug Res 2013;2:1-6.

Doughari J, Okafor B. Antimicrobial activity of Senna alata Linn. East

Cent Afr J Pharm Sci 2007;10:17-21.

Sukandar EY, Sunderam N, Fidrianny I. Activity of Kaempferia

pandurata (Roxb.) rhizome ethanol extract against MRSA, MRCNS,

MSSA, Bacillus subtilis and Salmonella typhi. Pak J Biol Sci

;17(1):1-7.

Baljeet SY, Simmy G, Ritika Y, Roshanlal Y. Antimicrobial activity

of individual and combined extracts of selected spices against

some pathogenic and food spoilage microorganisms. Int Food Res J

;22(6):2594-600.

Clinical and Laboratory Standards Institute (CLSI). Standards for

Antimicrobial Susceptibility Testing; Twenty-First Informational

Supplement. Vol. 31. USA: Clinical and Laboratory Standards Institute

(CLSI); 2011.

Clinical and Laboratory Standards Institute, M07-A08. Methods

Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow

Aerobically; Approve Standard. 8

ed. Pennsylvania: CLSI; 2009.

Eliopoulos GM, RC moelleringjr. Antimicrobial combinations. In: V

th

Lorian. ed, Antibiotics in Laboratory Medicines. 4thed. The Williams

& Wilkins Co, Baltimore, Md; 1996. p. 330-96.

Avila M, Saιd N, Ojcius DM. The book reopened on infectious diseases.

Microbes Infect 2008;10(9):942-7.

Miksusanti M, Jenie BS, Priosoeryanto BP, Syarief R, Rekso GT. Mode

of action Temu Kunci (Kaempferia pandurata) essential oil on E. coli

K1.1 cell determined by leakage of material cell and salt tolerance

assay. Hayati J Biosci 2008;15:56-60.

Bhamarapravati S, Juthapruth S, Mahachai W, Mahady G. Antibacterial

activity of Boesenbergia rotunda (L.) mansf. and Myristica fragrans

houtt. Against Helicobacter pylori. Songklanakarin J Sci Technol

;28:157-63.

Wikaningtyas P, Sukandar EY. The antibacterial activity study of Senna

alata leaf extract and fraction towards MRSA (methicillin resistant

Staphylococcus aureus) and its mode of action. World J Pharm Pharm

Sci 2015;4(4):126-33.

Rukayadi Y, Lee K, Han S, Yong D, Hwang JK. In vitro activities of

panduratin A against clinical Staphylococcus strains. Rob. Agents

Chemother 2009;53(10):4529-32.

Ncube B, Finnie JF, Van Staden J. In vitro antimicrobial synergism

within plant extract combinations from three South African medicinal

bulbs. J Ethnopharmacol 2012;139(1):81-9.

Adwan G, Mhanna M. Synergistic effect of plant extracts and antibiotics

on Staphylococcus aureus strains isolated from clinical specimens.

Asian Pac J Trop Med 2009;2(3):46-51.

Dunne Jr WM. Bacterial adhesion: Seen any good biofilms lately?. Clin

Microbiol Rev 2002;15(2):155-66.

Brooks JL, Jefferson KK. Staphylococcal biofilms. Quest for the magic

bullet. Adv Appl Microbiol 2012;81:63-87.

Published

01-05-2016

How to Cite

Wikaningtyas, P., and E. Y. Sukandar. “EVALUATION OF THE COMBINED ANTIBACTERIAL ACTIVITY OF KAEMPFERIA PANDURATA RHIZOME AND SENNA ALATA LEAVES AGAINST METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS”. Asian Journal of Pharmaceutical and Clinical Research, vol. 9, no. 3, May 2016, pp. 112-4, https://journals.innovareacademics.in/index.php/ajpcr/article/view/10340.

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