INVESTIGATION ON THE MECHANISM OF ACTION OF THE LEAVES OF TRIANTHEMA PORTULACASTRUM ON HUMAN PATHOGENS
Abstract
Objective: Many of the plants are used for medicinal purposes in human health needs. Trianthema portulacastrum has got many medicinal values and
also a dietary plant, being used in Ayurveda. It exhibits nephroprotective, diuretic, anti-cancerous, antihelmintic, antipyretic, analgesic properties,
anti-inflammatory activity, and also antibacterial activity. The objective of this study is to determine the mechanism of action of the leaves of
T. portulacastrum on human pathogens.
Methods: The mechanism has been analyzed by membrane permeability, time-kill kinetics, hemolytic activity, DNA binding ability, post antibiotic
effect, and bacterial membrane damage by scanning electron microscopy.
Results: This study reveal that the flavonoid and methanol fractions of T. portulacastrum have potent antibacterial activity against Staphylococcus
aureus and Klebsiella pneumoniae. The leaves of T. portulacastrum has been proved as a safer drug indicating its noncytotoxic property and found to
kill the bacterial cells by permeating the membrane and damage the DNA of the bacterial cells.
Conclusion: This study proved the mode of antibacterial action of T. portulacastrum on S. aureus and K. pneumoniae indicating its antibacterial
efficacy on human pathogens.
Keywords: Staphylococcus aureus, Postantibiotic, Klebsiella pneumoniae, Trianthema portulacastrum.
Downloads
References
REFERENCES
Racaniello VR. Emerging infectious diseases. J Clin Invest
;113(6):796-8.
Sirelkhatim A, Mahmud S, Seeni A, Kaus NH, Ann LC, Bakhori SK,
et al. Review on zinc oxide nanoparticles: Antibacterial activity and
toxicity mechanism. Nano-Micro Lett 2015;7(3):219-42.
Boeckel TP, Gandra S, Ashok A, Caudron Q, Grenfell BT, Levin SA,
et al. Global antibiotic consumption 2000 to 2010: An analysis of
national pharmaceutical sales data. Lancet Infect Dis 2014;14:70780-7.
Shaik G, Sujatha N, Mehar SK. Medicinal plants as source of
antibacterial agents to counter Klebsiella pneumoniae. J Appl Pharm
Sci 2014;4(01):135-47.
Jagatheeswari D, Deepa J, Ali HS, Ranganathan P. Acalypha indica L
an important medicinal plant: A review of its traditional uses, and
pharmacological properties. Int J Res Bot 2013;3(1):19-22.
Raja R, Sreenivasulu RM. Medicinal plants secondary metabolites used
in pharmaceutical importance - An overview. World J Pharm Pharm Sci
;4:436-47.
Upadhyay A, Upadhyaya I, Kollanoor-Johny A, Venkitanarayanan K.
Combating pathogenic microorganisms using plant-derived
antimicrobials: A mini review of the mechanistic basis. BioMed Res Int
;18:761741.
Lakshmi SK, Prabhakaran V, Mallikarjuna G, Gowthami A. Antilithiatic
activity of Trianthema portulacastrum l. and Gymnema sylvestre R.Br
against ethylene glycol induced urolithiasis. Int J Pharm Sci Rev Res
;25(1):16-22.
Yaron S, Rydlo T, Shachar D, Mor A. Activity of dermaseptin K4-S4
against foodborne pathogens. Peptides 2003;24(11):1815-21.
Park Y, Park S, Park H, Shin YS, Kim Y, Hahm K. Structure-activity
relationship of Hp(2-20) analog peptide: Enhanced antimicrobial
activity by N- terminal random coil region deletion. Pept Sci
;88:199-207.
Heipieper HJ, Diefenbach R, Kewelol H. Conversation of CIS
unsaturated fatty acids to trans, a possible mechanism for the protection
of phenol degrading Pseudomonas putida P8 from substrate toxicity.
Appl Environ Microbiol 1992;58:1847-52.
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement
with the Folin phenol reagent. J Biol Chem 1951;193(1):265-75.
Agyare C, Apenteng JA, Adu F, Kesseeih E, Boakye YD. Antimicrobial,
antibiotic resistance modulation and cytotoxicity studies of different
extracts of Pupalia lappacea. Pharmacologia 2015;6(6):244-57.
Hasan S, Danishuddin M, Khan AU. Inhibitory effect of Zingiber
officinale towards Streptococcus mutans virulence and caries
development: In vitro and in vivo studies. BMC Microbiol 2015;15:1.
Li L, Shi C, Yin Z, Jia R, Peng L, Kang S, et al. Antibacterial activity
of a-terpineol may induce morphostructural alterations in Escherichia
coli. Braz J Microbiol 2015;45(4):1409-13.
Gupta A, Chaphalkar SR. Use of flow cytometry to measure the
immunostimulatory activity of aqueous extract of Jasminum
auriculatum. Int J Curr Adv Res 2015;4:87-91.
Kumar G, Karthik L, Vankata L, Rao KV. Phytochemical
composition and in vitro antioxidant activity of aqueous extract of
Aerva lanata (L.) Juss. ex Schult. Stem (Amaranthaceae). Asian Pac J
Trop Med 2013;6(3):180-7.
Shabbir M, Khan MR, Saeed N. Assessment of phytochemicals,
antioxidant, anti lipid peroxidation and anti-hemolytic activity of
extract and various fractions of Maytenus royleanus leaves. BMC
Complement Altern Med 2013;13:143.
Karsha PV, Lakshmi OB. Antibacterial activity of black pepper (Piper
nigrum Linn.) with special reference to its mode of action on bacteria.
Indian J Nat Prod Resour 2010;1(2):213-5.
Vani KP, Lakshmi OB. In-vitro antibacterial efficacy of common spices
and their effect on permeability of membrane. Int J Pharm Bio Sci
;5(4):1203-11.
Asian J Pharm Clin Res, Vol 9, Issue 3, 2016, 135-140
Kavitha et al.
Anandhi D, Srinivasan PT, Kumar GP, Jagatheesh S. Influence
of flavonoids and glycosides from Caesalpinia coriaria (Jacq)
wild as bactericidal compound. Int J Curr Microbiol Appl Sci
b;3(4):1043-51.
Gurunathan S. Rapid biological synthesis of silver nanoparticles and
their enhanced antibacterial effects against Escherichia fergusonii and
Streptococcus mutans. Arabian J Chem 2014;7:143-701.
Al-Omair MA, Sayed AR, Youssef MM. Synthesis of novel triazoles,
tetrazine, thiadiazoles and their biological activities. Molecules
;20(2):2591-610.
Li H, Chen Q, Zhao J, Urmila K. Enhancing the antimicrobial activity
of natural extraction using the synthetic ultrasmall metal nanoparticles.
Sci Rep 2015;5:11033.
Ahmad I, Hao H, Huang L, Sanders P, Wang X, Chen D, et al. Integration
of PK/PD for dose optimization of Cefquinome against Staphylococcus
aureus causing septicemia in cattle. Front Microbiol 2015;6:588.
Hensler ME, Jang KH, Thienphrapa W, Vuong L, Tran DN, Soubih E,
et al. Anthracimycin activity against contemporary methicillin-resistant
Staphylococcus aureus. J Antibiot 2014;67(8):1-5.
Haste NM, Hughes CC, Tran DN, Fenical W, Jensen PR, Nizet V,
et al. Pharmacological properties of the marine natural product
marinopyrrole. A against methicillin-resistant Staphylococcus aureus.
Antimicrob Agents Chemother 2011;55(7):3305-12.
Tyagi P, Singh M, Kumari H, Kumari A, Mukhopadhyay K. Bactericidal
activity of Curcumin I is associated with damaging of bacterial
membrane. PLoS One 2015;10(3):e0121313.
Published
How to Cite
Issue
Section
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.