GREEN SYNTHESIS OF SILVER NANOBIOCONJUGATES FROM PIPER BETLE LEAVES AND ITS ANTICANCER ACTIVITY ON A549 CELLS

Abstract

ABSTRACT
Objective: This study focused on the anticancer effect of silver nanobioconjugates synthesis from betel leaf extract and its major component eugenol
(EU) and its non-conjugated form.
Methods: The silver nanobioconjugates were synthesized from betel leaf extract and its active compound EU under sunlight exposure. The anticancer
activity of nanobioconjugates was tested and reported in lung adenocarcinoma (A549 cell line). The non-toxicity of the conjugates was further affirmed
in human peripheral blood lymphocytes as non-cancerous cells. The anticancer activity was analyzed by cell viability (3(4,5-dimethly-thiazol-2-yl)2,5-diphenyl
tetrazolium
bromide
assay)
and staining techniques
(acridine
orange/ethidium
bromide),
which
were
carried out
in both the cancerous

and
non-cancerous
cells. The phase of cell death was
analyzed
by
flow
cytometry.
Results: The activity of nanobioconjugates was mediated by apoptosis as an evidence of low viability and nuclear fragmentation in lung cancer cell
line. The cell cycle analysis also confirmed the efficiency of silver nanobioconjugates; the data reveal that the phenolic compound of betel leaves has
potent anticancer activity against the cancer cells in the form of nano, when compared with the non-conjugates.
Conclusion: The study demonstrated the synthesized silver nanobioconjugates have potent anticancer activity than their non-nano form.
Keywords: Betel leaves, Eugenol, Silver nanoparticles, Anticancer, A549 cells.