ANALYSIS OF PROTEIN ISOLATE FROM QUINOA (CHENOPODIUM QUINOA WILLD)
Abstract
ABSTRACT
Objective: The aim of this study was to obtain protein isolate from quinoa using alkaline pH at different pHs of precipitation and to analyze protein
isolate with electrophoresis.
Methods: Quinoa protein isolates were obtained using isoelectric precipitation method at different pHs. Proteins were analyzed using electrophoresis
native-polyacrylamide gel electrophoresis (PAGE) and sodium dodecyl sulfate - PAGE.
Results: A yield of 6.29% of protein isolate of defatted quinoa at pH 4.0 was obtained. The content of protein isolate was higher than 64% in all
pH assays. Globulins and albumins in protein isolate at different pHs were observed. One band near 130 kDa was found. A band with MW 60 kDa
corresponding to 7S globulin was found. The bands, MW 33-36 kDa and MW 20-22 kDa, correspond to 11S globulin. Bands less to 15.4 kDa correspond
to albumins.
Conclusions: Quinoa is a good source of proteins. Globulins and albumins were identified in the quinoa protein isolate.
Keywords: Quinoa, Globulins, Albumins, Polypeptides, Protein isolate.
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REFERENCES
Hirose Y, Fujita T, Ishii T, Ueno N. Antioxidative properties and
flavonoid composition of Chenopodium quinoa seeds cultivated in
Japan. Food Chem 2010;119:1300-6.
Elsohaimy SA, Refaay TM, Zaytoun MA. Physico chemical and
functional properties of quinoa protein isolate. Ann Agric Sci
;60:297-305.
Alvarez-Jubete L, Arendt EK, Gallagher E. Nutritive value of
pseudocereals and their increasing use as functional gluten-free
ingredients. Trends Food Sci Technol 2010;21:106-13.
Vega-Gálvez A, Miranda M, Vergara J, Uribe E, Puente L,
MartÃnez EA. Nutrition facts and functional potential of quinoa
(Chenopodium quinoa Willd.), an ancient Andean grain: A review. J Sci
Food Agric 2010;90:2541-7.
Abugoch LE, Romero N, Tapia CA, Silva J, Rivera M. Study of some
physicochemical and functional properties of quinoa (Chenopodium
quinoa Willd) protein isolates. J Agric Food Chem 2008;56:4745-50.
AOAC. Official Methods of Analysis. Washington, DC, USA:
Association of Official Analytical Chemists; 2012.
MartıÃnez NE, Añón MC. Composition and structural characterization
of amaranth protein isolates. An electrophoretic and calorimetric study.
J Agric Food Chem 1996;44:2523-30.
Gornall AG, Bardawill CJ, David MM. Determination of serum proteins
by means of the biuret reaction. J Biol Chem 1949;177:751-66.
Laemmli UK. Cleavage of structural proteins during the assembly of
the head of bacteriophage T4. Nature 1970;227:680-5.
Karyotis T, Iliadis C, Noulas C, Mitsibonas T. Preliminary research on
seed production and nutrient content for certain quinoa varieties in a
saline-sodic. Soil J Agron Crop Sci 2003;189:402-8.
Abugoch JL. Quinoa (Chenopodium quinoa Willd.): Composition,
chemistry, nutritional, and functional properties. Adv Food Nutr Res
;58:1e31.
Kozioł M. Chemical composition and nutritional evaluation of quinoa
(Chenopodium quinoa Willd). J Food Compost Anal 1992;5:35-68.
Brinegar C, Sine B, Nwokocha L. High-cysteine 2S seed storage
proteins from quinoa (Chenopodium quinoa). J Agric Food Chem
;44:1621-3.
Brinegar C, Goundan S. Isolation and characterization of chenopodin,
the 11S seed storageprotein of quinoa (Chenopodium quinoa). J Agric
Food Chem 1993;41:182-5.
Srivastava R, Roy B. Proteomic analysis of different extracts from
amaranth (Amaranthus tricolor) grains. Asian J Pharm Clin Res
;6:37-9.
Romero-Zepeda H, Paredes-Lopez O. Isolation and characterizaton
of amaranthin. The 11s Amaranth seed globulin. J Food Chem
;19:329-39
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