DIGESTIBILITY AND ANTI-INFLAMMATORY ACTIVITY IN VITRO OF SACHA INCHI (PLUKENETIA VOLUBILIS L.) PROTEINS

Authors

Abstract

Objective: The aim of this study was to evaluate the digestibility and anti-inflammatory activity in vitro of sacha inchi protein isolate.
Methods: Proteins were analyzed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Gastric digestibility was evaluated with pepsin
at different pHs and at different relation enzyme/substrate. Anti-inflammatory activity in vitro of sacha inchi protein isolate was evaluated using
denatured protein method with egg albumin.
Results: A yield of 20.88% of protein isolate of defatted sacha inchi flour at pH 4.0 with a 93.1% of protein was obtained. 11S globulins were resistant
to gastric and duodenal digestion. Sacha inchi protein isolate at pH 4.0 (1000 µg/ml) presents 78.13% of anti-inflammatory activity in vitro.
Conclusions: Sacha inchi seed is a good source of proteins. 11S globulins are resistant to pepsin and pancreatin hydrolysis. Sacha inchi protein isolate
has high anti-inflammatory activity in vitro.
Keywords: Sacha inchi, Gastric digestion, Duodenal digestion, Globulins and albumins.

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Author Biography

CARRILLO W, Universidad Técnica de Ambato

Facultad de Ciencia e Ingeería en Aliementos

References

Sathe SK, Hamaker BR, Sze-Tao KW, Venkatachalam M. Isolation,

purification, and biochemical characterization of a novel water soluble

protein from Inca peanut (Plukenetia volubilis L.). J Agric Food Chem

;50(17):4906-8.

Mao X, Hua Y, Chen G. Amino acid composition, molecular weight

distribution and gel electrophoresis of walnut (Juglans regia L.)

proteins and protein fractionations. Int J Mol Sci 2014;15(2):2003-14.

Mao XY, Hua YF. Chemical composition, molecular weight distribution,

secondary structure and effect of NaCl on functional properties of

walnut (Juglans regia L) protein isolates and concentrates. J Food Sci

Technol 2014;51(8):1473-82.

Czubinski J, Dwiecki K, Siger A, Kachlicki P, Neunert G,

Lampart-Szczapa E, et al. Release of flavonoids from lupin globulin

proteins during digestion in a model system. J Agric Food Chem

;60(7):1830-6.

Sathe SK, Kshirsagar HH, Sharma GM. Solubilization, fractionation, and

electrophoretic characterization of Inca peanut (Plukenetia volubilis L.)

proteins. Plant Foods Hum Nutr 2012;67(3):247-55.

Fanali C, Dugo L, Cacciola F, Beccaria M, Grasso S, Dachà M, et al.

Chemical characterization of Sacha Inchi (Plukenetia volubilis L.)

oil. J Agric Food Chem 2011;59(24):13043-9.

Alhakmani F, Kumar S, Khan SA. Estimation of total phenolic content,

in vitro antioxidant and anti–inflammatory activity of flowers of

Moringa oleifera. Asian Pac J Trop Biomed 2013;3:623-7.

Padmanabhan P, Jangle S.N. Evaluation of in-vitro anti-inflammatory

activity of herbal preparation, a combination of four medicinal plants.

Int J Basic App Med Scie 2012;1:109-16.

Chandra S, Chatterjee P, Dey P, Bhattacharya S. Evaluation of in vitro

anti-inflammatory activity of coffee against the denaturation of protein.

Asian Pac J Trop Biomed 2012;2:S178-S80.

Sabina EP, Rasool M. An in vivo and in vitro potential of Indian

ayurvedic herbal formulation Triphala on experimental gouty arthritis

in mice. Vascul Pharm 2008;48:14-20.

Martıínez NE, Añón MC. Composition and structural characterization

of amaranth protein isolates. An electrophoretic and calorimetric study.

J Agric Food Chem 1996;44:2523-30.

Carrillo W, García-Ruiz A, Recio I, Moreno-Arribas MV. Antibacterial

activity of hen egg white lysozyme modified by heat and enzymatic

treatments against oenological lactic acid bacteria and acetic acid

bacteria. J Food Prot 2014;77(10):1732-9.

Jiménez-Saiz R, Martos G, Carrillo W, López-Fandiño R, Molina E.

Susceptibility of lysozyme to in-vitro digestion and immunoreactivity

of its digests. Food Chem 2011;127:1719-26.

Martos G, Contreras P, Molina E, López-Fandiño R. Egg white

ovalbumin digestion mimicking physiological conditions. J Agric Food

Chem 2010;58(9):5640-8.

Laemmli UK. Cleavage of structural proteins during the assembly of

Asian J Pharm Clin Res, Vol 9, Issue 3, 2016, 303-306

Quinteros et al.

the head of bacteriophage T4. Nature 1970;227(5259):680-5.

Padmanabhan P, Jangle SN. Evaluation of in-vitro anti-inflammatory

activity of herbal preparation, a combination of four medicinal plants.

Int J Basic Appl Med Sci 2012;2:109-16.

Chandra S, Chatterjee P, Dey P, Bhattacharya S. Evaluation of in vitro

anti-inflammatory activity of coffee against the denaturation of protein.

Asian Pac J Trop Biomed 2012;2012:S178-80.

Romero-Zepeda H, Paredes-Lopez O. Isolation and characterizaton

of amaranthin. The 11S amaranth seed globulin. J Food Chem

;19:329-39.

Wolf WJ. Purification and properties of the proteins. In: Smith AK,

Circle SJ, editors. Soybean: Chemistry and Technology. Proteins.

Vol. 1. New York: Van Nostrand Reinhold AVl; 1980. p. 93-115.

Czubinski J, Dwiecki K, Siger A, Neunert G, Lampart-Szczapa E.

Characterisation of different digestion susceptibility of lupin seed

globulins. Food Chem 2014;143:418-26.

Brinegar C, Sine B, Nwokocha L. High-cysteine 2S seed storage

proteins from quinoa (Chenopodium quinoa). J Agric Food Chem

;44:1621-3.

Carbonaro M, Maselli P, Nucara A. Structural aspects of legume

proteins and nutraceutical properties. Food Res Int 2015;76:19-30.

Carbonaro M. 7S globulins from Phaseolus vulgaris L.: Impact of

structural aspectson the nutritional quality. Biosci Biotechnol Biochem

;70:2620-6.

Carbonaro M, Grant G, Cappelloni M. Heat-induced denaturation

impairs digestibility of legume (Phaseolus vulgaris L. and Vicia faba L.)

S and 7S globulins in the small intestine of rat. J Sci Food Agric

;85:65-72.

Faris RJ, Wang H, Wang T. Improving digestibility of soy flour

by reducing disulfide bonds with thioredoxin. J Agric Food Chem

;56:7146-50.

Lee HW, Keum EH, Lee SJ, Sung DE, Chung DH, Lee SI, et al.

Allergenicity of proteolytic hydrolysates of the soybean 11S globulin. J

Food Sci 2007;72(3):C168-72.

Published

01-05-2016

How to Cite

MF, Q., V. R, C. C, and C. . W. “DIGESTIBILITY AND ANTI-INFLAMMATORY ACTIVITY IN VITRO OF SACHA INCHI (PLUKENETIA VOLUBILIS L.) PROTEINS”. Asian Journal of Pharmaceutical and Clinical Research, vol. 9, no. 3, May 2016, pp. 303-6, https://journals.innovareacademics.in/index.php/ajpcr/article/view/11370.

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