• Vineet Mehta
  • Arun Sharma
  • Pallavi Kailkhura
  • Udayabanu Malairaman Jaypee University of Information Technology, Waknaghat, Himachal Pradesh, India-173234.



Objective: Ocimum sanctum is known to be beneficial in the management of diabetes, however, its mechanism remains unexplored. This study was
aimed to gain insite into the mechanisms through which it may counter diabetes and its complications.

Methods: Hydroalcoholic whole plant extract of O. sanctum was screened for its antidiabetic potential and ability to counter oxidative and
inflammatory stress through various in-vitro assays. Further, bioactive compounds that may be responsible for its antidiabetic activity were predicted
through molecular-docking studies.

Results: Crude extractive yield of 35.43% was obtained from Soxhlet extraction which mainly showed the presence of flavonoids, alkaloids, glycosides, and saponins. Plant extract showed good potential to scavenge 2,2-diphenyl-1-picrylhydrazyl free radical (40.95-68.71%) which may be attributed\ to its high phenolic (0.366 mg gallic acid equivalent/g) and flavonoid (0.113 mg quercetin equivalent/g) contents. Plant showed exceptional antiinflammatory activity which was evaluated through inhibition of protein denaturation (47.61-82.37%) and red blood cell membrane stabilization assay (43.66-78.28%). Further, extract treatment greatly inhibited α-glucosidase enzyme (34.17-71.45%) but failed to produce noticeable inhibition of α-amylase activity (1.94-14.88%). Docking studies predicted that rosmarinic acid, stigmasterol, linalool, bieugenol, and aesculin may be responsible for antidiabetic activity possessed by the plant through their interaction with the insulin receptor.

Conclusion: These findings conclude that O. sanctum may be beneficial in managing diabetes and its associated complications through inhibiting
α-glucosidase activity, reducing oxidative and inflammatory stress.

Keywords: Diabetes mellitus, Ocimum sanctum, Antioxidant, Anti-inflammatory, Docking, Diabetic complications.


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Author Biography

Udayabanu Malairaman, Jaypee University of Information Technology, Waknaghat, Himachal Pradesh, India-173234.

Udayabanu Malairaman, Assistant Professor,Jaypee University of Information Technology, Waknaghat, Himachal Pradesh, 173234, India.


International Diabetes Federation. The Diabetes Atlas. 7th ed. Brussels: International Diabetes Federation; 2015.

Cade WT. Diabetes-related microvascular and macrovascular diseases in the physical therapy setting. Phys Ther 2008;88(11):1322-35.

Mehta V, Malairaman U. Flavonoids: Prospective strategy for the management of diabetes and its associated complications. In: Wang V, editor. Handbook of Research on Advancing Health Education through Technology. Pennsylvania: IGI Global; 2006. p. 286-328.

Kolb H, Mandrup-Poulsen T. The global diabetes epidemic as a consequence of lifestyle-induced low-grade inflammation. Diabetologia 2010;53(1):10-20.

Bondia-Pons I, Ryan L, Martinez JA. Oxidative stress and inflammation interactions in human obesity. J Physiol Biochem 2012;68(4):701-11.

Winter WE, Signorino MR. Diabetes Mellitus: Pathophysiology, Etiologies, Complications, Management, and Laboratory Evaluation: Special Topics in Diagnostic Testing. Washington: American Association for Clinical Chemistry Press; 2002.

Gordon MC, David JN. Natural product drug discovery in the next millennium. Pharm Boil 2001;39:8-17.

Prakash P, Gupta N. Therapeutic uses of Ocimum sanctum Linn (Tulsi) with a note on eugenol and its pharmacological actions: A short review. Indian J Physiol Pharmacol 2005;49(2):125-31.

Modak M, Dixit P, Londhe J, Ghaskadbi S, Devasagayam TP. Indian herbs and herbal drugs used for the treatment of diabetes. J Clin Biochem Nutr 2007;40(3):163-73.

Patil RN, Patil RY, Ahirwar B, Ahirwar D. Evaluation of antidiabetic and related actions of some Indian medicinal plants in diabetic rats. Asian Pac J Trop Med 2011;4(1):20-3.

Chattopadhyay RR. A comparative evaluation of some blood sugar lowering agents of plant origin. J Ethnopharmacol 1999;67(3):367-72.

Hussain EH, Jamil K, Rao M. Hypoglycaemic, hypolipidemic and antioxidant properties of tulsi (Ocimum sanctum linn) on streptozotocin induced diabetes in rats. Indian J Clin Biochem 2001;16(2):190-4.

Sethi J, Sood S, Seth S, Talwar A. Evaluation of hypoglycemic and antioxidant effect of Ocimum sanctum. Indian J Clin Biochem 2004;19(2):152-5.

Gupta S, Mediratta PK, Singh S, Sharma KK, Shukla R. Antidiabetic, antihypercholesterolaemic and antioxidant effect of Ocimum sanctum (Linn) seed oil. Indian J Exp Biol 2006;44(4):300-4.

Hannan JM, Marenah L, Ali L, Rokeya B, Flatt PR, Abdel-Wahab YH. Ocimum sanctum leaf extracts stimulate insulin secretion from perfused pancreas, isolated islets and clonal pancreatic beta-cells. J Endocrinol 2006;189(1):127-36.

Husain I, Chander R, Saxena JK, Mahdi AA, Mahdi F. Antidyslipidemic effect of Ocimum sanctum leaf extract in streptozotocin induced diabetic rats. Indian J Clin Biochem 2015;30(1):72-7.

Halim EM, Mukhopadhyay AK. Effect of Ocimum sanctum (Tulsi) and vitamin E on biochemical parameters and retinopathy in streptozotocin induced diabetic rats. Indian J Clin Biochem 2006;21(2):181-8.

Khandelwal KR. Practical Pharmacognosy: Techniques and Experiments. 17th ed. Pune: Nirali Prakashan; 2007.

Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic 1965;16(3):144-58.

Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 1999;64:555-9.

Sharma OP, Bhat TK. DPPH antioxidant assay revisited. Food Chem 2009;113:1202-5.

Ullah HM, Zaman S, Juhara F, Akter L, Tareq SM, Masum EH, et al. Evaluation of antinociceptive, in-vivo & in-vitro anti-inflammatory activity of ethanolic extract of Curcuma zedoaria rhizome. BMC Complement Altern Med 2014;14:346.

Asanuma M, Taguchi C, Kumagi T, Uesaka H, Hosokawa H, Kuriya SI. The hydrogen ion concentration (pH) in blood samples with K-2EDTA and K-3EDTA affects mean corpuscular volume values in hemodialysis patients. Lab Hematol 2000;6:67-72.

Sadique J, Al-Rqobah W, Bughaith M, El-Gindy A. The bio-activity of certain medicinal plants on the stabilization of RBC membrane system. Fitoterapia 1989;60:525-32.

Shinde UA, Phadke AS, Nair AM, Mungantiwar AA, Dikshit VJ, Saraf MN. Membrane stabilizing activity-a possible mechanism of action for the anti-inflammatory activity of Cedrus deodara wood oil. Fitoterapia 1999;70(3):251-7.

Elya B, Basah K, Mun’im A, Yuliastuti W, Bangun A, Septiana EK. Antidiabetic activity test by inhibition of α-glucosidase and phytochemical screening from the most active fraction of Buni (Antidesma bunius L.) stem barks and leaves. J Biomed Biotechnol 2011;2012:281078.

Zengin G, Uysal A, Gunes E, Aktumsek A. Survey of phytochemical composition and biological effects of three extracts from a wild plant (Cotoneaster nummularia Fisch. et Mey.): A potential source for functional food ingredients and drug formulations. PLoS One 2014;9(11):e113527.

Halgern TA. A new prospective in the docking analysis. Comput Chem 1990;20:720-7.

Hasel WH, Hendrickson TF, Still WC. A rapid approximation to the solvent accessible surface areas of atoms. Tetrahedron Comput Methodol 1988;1:103-16.

Jayaram B, Sprous D, Beveridge DL. Solvation free energy of biomacromolecules: Parameters for a modified generalized born model consistent with the AMBER force field. J Phys Chem 1998;B102:9571-6.

Saso L, Valentini G, Casini ML, Grippa E, Gatto MT, Leone MG, et al. Inhibition of heat-induced denaturation of albumin by nonsteroidal antiinflammatory drugs (NSAIDs): Pharmacological implications. Arch Pharmacal Res 2001;24(2):150-8.

Shahidi F, Wanasundara PK. Phenolic antioxidants. Crit Rev Food Sci Nutr 1992;32(1):67-103.

Ceriello A, Motz E. Is oxidative stress the pathogenic mechanism underlying insulin resistance, diabetes, and cardiovascular disease? The common soil hypothesis revisited. Arterioscler Thromb Vasc Biol 2004;24(5):816-23.

Tripathi KD. Essentials of medical pharmacology. 7th ed. New Delhi: JP Medical Ltd; 2013.

Anosike CA, Obidoa O, Ezeanyika LU. Membrane stabilization as a mechanism of the anti-inflammatory activity of methanol extract of garden egg (Solanum aethiopicum). Daru J Pharm Sci 2012;20(1):76.

Forbes JM, Coughlan MT, Cooper ME. Oxidative stress as a major culprit in kidney disease in diabetes. Diabetes 2008;57(6):1446-54.

Vangrevelinghe E, Zimmermann K, Schoepfer J, Portmann R, Fabbro D, Furet P. Discovery of a potent and selective protein kinase CK2 inhibitor by high-throughput docking. J Med Chem 2003;46(13):2656-62.

Rollinger JM, Steindl TM, Schuster D, Kirchmair J, Anrain K, Ellmerer EP, et al. Structure-based virtual screening for the discovery of natural inhibitors for human rhinovirus coat protein. J Med Chem 2008;51(4):842-51.



How to Cite

Mehta, V., A. Sharma, P. Kailkhura, and U. Malairaman. “ANTIOXIDANT, ANTI-INFLAMMATORY, AND ANTIDIABETIC ACTIVITY OF HYDROALCOHOLIC EXTRACT OF OCIMUM SANCTUM: AN IN-VITRO AND IN-SILICO STUDY”. Asian Journal of Pharmaceutical and Clinical Research, vol. 9, no. 5, Sept. 2016, pp. 44-49, doi:10.22159/ajpcr.2016.v9i5.12713.



Original Article(s)