ANTIFERTILITY ACTIVITY AND CONTRACEPTIVE POTENTIAL OF THE HYDROALCOHOLIC RHIZOME EXTRACT OF TRILLIUM GOVANIANUM IN FEMALE WISTAR RATS

Shivam Sharma; Vineet Mehta; Parul Sharma; Kritika Jaggi; Malairaman Udayabanu; Hemant Sood

doi:10.22159/ajpcr.2018.v11i11.27420

Authors

Shivam Sharma Department of Bioinformatics and Biotechnology, Jaypee University of Information Technology, Waknaghat, Solan - 173 234, Himachal Pradesh, India.
Vineet Mehta Department of Pharmacy, Jaypee University of Information Technology, Waknaghat, Solan - 173 234, Himachal Pradesh, India.
Parul Sharma Department of Bioinformatics and Biotechnology, Jaypee University of Information Technology, Waknaghat, Solan - 173 234, Himachal Pradesh, India.
Kritika Jaggi Department of Bioinformatics and Biotechnology, Jaypee University of Information Technology, Waknaghat, Solan - 173 234, Himachal Pradesh, India.
Malairaman Udayabanu Department of Pharmacy, Jaypee University of Information Technology, Waknaghat, Solan - 173 234, Himachal Pradesh, India.
Hemant Sood Department of Bioinformatics and Biotechnology, Jaypee University of Information Technology, Waknaghat, Solan - 173 234, Himachal Pradesh, India.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i11.27420

Keywords:

Antifertility, Trillium govanianum, Estrogen, Anti-implantation, Steroids, Herbal remedy

Abstract

Objective: Trillium govanianum is used in several traditional containing steroids and sex hormones for the management of inflammation, menstrual disorders, sex-related disorders, and antiseptic. The present study was aimed to investigate the antifertility potential of hydroalcoholic rhizome extract of T. govanianum and to explore the possible mechanism of action.

Methods: Anti-implantation activity of T. govanianum rhizome extract (125 and 250 mg/kg; p.o.) was performed in female Wistar rats with proven fertility, and its estrogenic/antiestrogenic effect was evaluated in ovariectomized females. 17-Î±-ethinylestradiol (1 Î¼g/rat/day; s.c.) or plant extract was administered for 11 days after which animals were sacrificed. Percentage inhibition of implantation sites, serum estrogen levels, changes in body and uterus weight, and morphological alterations in the uterus and ovaries were evaluated.

Results: T. govanianum treatment resulted in increased uterus weight and induced dose-dependent anti-implantation effect, with 100% implantation inhibition at 250 mg/kg dose. Anti-implantation effects of T. govanianum were associated with endometrial thickening and significantly elevated serum estrogen levels. Moreover, estrogenic/antiestrogenic studies revealed that T. govanianum possessed strong estrogenic effect; however, the effect was saturable.

Conclusion: T. govanianum possesses antifertility activity which can be attributed to its strong estrogenic potential and uterine thickening. Moreover, it could find a clinical application as a safer and efficacious birth control herbal remedy.

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