FINGER PRINTING ANALYSIS OF THE PHYTOSTEROLS FROM Holoptelea Integrifolia (Roxb.) PLANCH LEAVES USING HPTLC ANALYSIS
Abstract
Objective: The present study was conducted to identify the phytosterols from petroleum ether and methanol extracts of medicinally and economically
useful leaves of Holoptelea integrifolia (Roxb.) Planch using high-performance thin layer chromatography (HPTLC) technique.
Materials and Methods: Preliminary phytochemical screening was done and HPTLC studies were carried out. Camag HPTLC system equipped with
Linomat V applicator (Switzerland). Densitometric scanning was performed with Camag thin layer chromatography scanner IV in the reflectance
absorbance mode at 540 nm and operated by Win CATS software (1.4.6 Camag) with the help of tungsten lamp.
Results: Preliminary phytochemical screening of petroleum ether extract of H. integrifolia showed the presence of steroids, terpenoids, alkaloids,
glycosides, flavonoids, proteins, tannins, and carbohydrates while methanolic extract of H. integrifolia showed the presence of steroids, alkaloids,
flavonoids, proteins, and carbohydrates. HPTLC fingerprinting of phytosterols of petroleum ether extract of leaf revealed five polyvalent
phytoconstituents (5 peaks) and corresponding ascending order of Rf values in the range of 0.11-0.45. While methanol extract of leaf showed four
polyvalent phytoconstituents (4 peaks) and corresponding ascending order of Rf values in the range of 0.13-0.48.
Conclusions: With the results of preliminary phytochemical analysis and above Rf values, we have concluded the presence of phytosterols in both
the extracts.
Keywords: High-performance thin layer chromatography fingerprinting, Holoptelea integrifolia (Roxb.) planch leaf, Phytochemical screening,
Phytosterol.
Downloads
References
Prabavathy D, Valli Nachiyar C. Antimicrobial and antidiabetic activity
of an endophytic fungi isolated from Adathoda beddomei. Int J Pharm
Pharm Sci 2013;5(3):???.
el-Mousallamy AM. Leaf flavonoids of Albizia lebbeck. Phytochemistry
;48(4):759-61.
Nazneen Bobby MD, Wesely EG, Johnson M. High performance
thin layer chromatography profile studies on the alkaloids of Albizia
lebbeck. Asian Pac J Trop Biomed 2012;2(1):S1-6.
Nadkarni KM. Indian Materia Medica. Mumbai, India: Popular
Prakashan Pvt. Ltd.; 1976. p. 651-2.
Kirtikar KR, Basu BD. Indian Medicinal Plants. 3rd ed., Vol. III. New
Delhi, India: Sri Satguru Publications; 2000. p. 2292-4.
Prajapati ND, Purohit SS, Sharma AK. A Handbook of Medicinal Plants
a Complete Source Book. Jodhpur, India: Agrobias; 2003. p. 273.
Warrier PK, Nambiar VP, Ramakutty C. Indian Medicinal Plants a
Compendium of 500 Species. Vol. 3. Hyderabad: Orient Longman
Private Limited.; 1995. p. 162.
Khandelwal KR. Practical Pharmacognosy. Techniques and
Experiments. 10th ed. Pune, India: Nirali Prakashan; 2006. p. 149-56.
Harborne JB. Phytochemical Methods. 3rd ed. London: Chapman and
Hall; 1998.
Wagner H, Baldt S. Plant Drug Analysis. Berlin: Springer; 1996.
Cazes J, Scott RP. Chromatography Theory. NY: Marcel Decker; 2002.p. 443-54.
ICH/CPMP Guidelines Q2B, Validation of Analytical ProceduresMethodology; 1996.
Reviewer Guidance, Validation of Chromatographic Methods; 1994.
Sethi PD. HPTLC: Quantitative Analysis ofFormulations. New Delhi: CBS Publications; 1996. p. 162-5.
Heftman E. Chromatography Fundamentals and Applications of
Chromatography and Related Differential Migration Methods. 6th ed.,
Vol. 69A. Amsterdam: Elsevier; 2004. p. 253-91.
British Pharmacopoeia. International ed., Vol. II. Cambridge: HMSO;
Appendix 112 (IB).
Sherma J. Encyclopedia of Pharmaceutical Technology. 2nd ed. USA:
Marcel Dekker; 2001. p. 252-4.
ICH/CPMP Guidelines Q2A, Text on Validation of Analytical
Procedures; 1994.
USP 23, NF 19, Asian ed. Rockville, M.D: United States
Pharmacopeial Convention; 1982. p. 1225.
Hariprasad PS, Ramakrishnan N. HPTLC fingerprint profile of Rumex
Vesicarius L. Asian J Pharm Clin Res 2011;4(2):134-6.
Swarnalatha Y, Kommineni L. Finger printing analysis of the alkaloids
from Sphaeranthus amaranthoides leaves using HPTLC analysis.
Asian J Pharm Clin Res 2014;7(2):125-7.
Crofford LJ, Wilder RL. Arthritis and autoimmunity in animals. In:
McCarty DJ, Koopman WJ, editors. Arthritis and Allied Conditions.
London: Lea and Febiger; 1993. p. 525-39.
Walz DT, DiMartino MJ, Misher A. Adjuvant-induced arthritis in
rats. II. Drug effects on physiologic, biochemical and immunologic
parameters. J Pharmacol Exp Ther 1971;178(1):223-31.
Bouic PJ. Sterols/sterolins: The natural, nontoxic immuno-modulators and
their role in the control of rheumatoid arthritis. Arthritis Trust 1998;???:3-6.
Gupta MB, Nath R, Srivastava N, Shanker K, Kishor K, Bhargava KP.
Anti-inflammatory and antipyretic activities of beta-sitosterol. PlantaMed 1980;39(2):157-63.
Bouic PJ, Etsebeth S, Liebenberg RW, Albrecht CF, Pegel K, Van
Jaarsveld PP. Beta-sitosterol and beta-sitosterol glucoside stimulate
human peripheral blood lymphocyte proliferation: Implications
for their use as an immunomodulatory vitamin combination. Int J
Immunopharmacol 1996;18(12):693-700.
Aguirre-Hernández E, Rosas-Acevedo H, Soto-Hernández M,
MartÃnez AL, Moreno J, González-Trujano ME. Bioactivity-guided
isolation of beta-sitosterol and some fatty acids as active compounds
in the anxiolytic and sedative effects of Tilia americana var. mexicana.
Planta Med 2007;73(11):1148-55.
Published
How to Cite
Issue
Section
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.