PHYTOCHEMICAL PROFILING AND EFFICACY EVALUATION OF ANTIBACTERIAL POTENTIAL OF MYRISTICA FRAAGRANS SEED EXTRACTS AGAINST SELECTED MICROORGANISMS
DOI:
https://doi.org/10.22159/ajpcr.2016.v9i6.14287Abstract
Introduction: Traditional plants have been proved as novel source for health care remedies. Plants are the rich source of bioactive compounds which can be useful for health care purpose. Emergence of resistance among pathogenic bacteria against available antibiotics is a common phenomenon. So the plant based bioactive molecule may be an alternative for the development of new formulation. The use of natural bioactive molecules as parental compound for development of new formulation may be safer with less side effects as compared to synthetic chemical compounds. Myristica fraagrans seed are widely used in routine life from ancient time for health care. Traditionally it is being used in for stomach related complications. So the current study pertained to the phytochemical profiling of Myristica fraagrans seed and its efficacy evaluation against some clinically significant bacterial species.
Methods: Â In this study ethanol and acetone extract of Myristica fraagrans seed (Jaiphal) were prepared via using soxhlet apparatus. Extract were qualitatively analysed to check the phytochemical composition. Then antibacterial efficacy of prepared extracts were analysed by agar well diffusion assay against Escherichia coli, Bacillus subtilis, Bacillus Magetarium, Proteus mirabilis.
Results: The result of phytochemical profiling shows the presence of tannin, terpenoids, alkaloids, indole and phenol. Moreover Myristica fraagrans seed extract exhibiting significant antibacterial activity against selected microorganisms.
Conclusion: Finding of the study concludes that Myristica fragrans seed ( jiphal )Â possess the antimicrobial capabilities against infections and/or diseases caused by chosen microorganisms and seems to be useful in the discovery of novel antibiotic drugs.
Â
KEY WORDS: Myristica fraagrans (Jaiphal), Escherichia coli., Bacillus subtilis, Bacillus Magetarium, Proteus mirabilis., antibacterial activity etc.
Â
Downloads
References
REFERENCES
Ayoola GA, Lawore FM, Adelowotan T, Aibinu IE, Adenipekun E,
Coker HA, et al. Chemical analysis and antimicrobial activity of the
essential oil of Syzigium aromaticum (clove). Afr J Microbiol Res
;2:162-6.
Nimje PD, Garg H, Gupta A, Srivastava N, Katiyar M, Ramalingam C.
Comparison of antimicrobial activity of Cinnamomum zeylanicum
and Cinnamomum cassia on food spoilage bacteria and water borne
bacteria. Der Pharm Lett 2013;5(1)`53-9.
Patil SD, Shinde S, Kandpile P, Jain AS. Evaluation of antimicrobial
activity of asafoetida. Int J Pharm Sci Res 2014;6(2):722-7.
Shete HG, Chitanand MP. Antimicrobial activity of some commonly
used Indian Spices. Int J Curr Microbiol Appl Sci 2014;3(8):765-70.
Dua A, Garg G, Mahajan R. Polyphenols, flavonoids and antimicrobial
properties of methanolic extract of fennel (Foeniculum vulgare Miller).
Eur J Exp Biol 2013;3(4):203-8.
Shelef LA. Antimicrobial effects of spices. J Food Saf 1983;6(1):29-44.
Chattopadhyay I, Biswas K, Bandyopadhyay U, Banerjee RK. Turmeric
and curcumin: Biological actions and medicinal applications. Curr Sci
;87(1):44-53.
Choi EM, Hwang JK. Antiinflammatory, analgesic and antioxidant
activities of the fruit of Foeniculum vulgare. Fitoterapia 2004;75:557-65.
Encyclopedia Britannica. Available from: https://www.britannica.com/
topic/nutmeg.
Hussein G, Miyashiro H, Nakamura N, Hattori M, Kakiuchi N,
Shimotohno K. Inhibitory effects of Sudanese medicinal plant
extracts on hepatitis C virus (HCV) protease. Phytother Res
;14(7):510-6.
Ahmadiani A, Javan M, Semnanian S, Barat E, Kamalinejad M. Antiinflammatory
and antipyretic effects of Trigonella foenum-graecum
leaves extract in the rat. J Ethnopharmacol 2001;75(2-3):283-6.
Grover JK, Yadav S, Vats V. Medicinal plants of India with anti-diabetic
potential. J Ethnopharmacol 2002;81(1):81-100.
Irobi ON, Moo-Young M, Anderson WA, Daramola SO. Antimicrobial
activity of the bark of Bridelia fermginea (Euphorbiaceae). Int J
Pharmacogn 1994;34:87-90.
Cowan MM. Plant products as antimicrobial agents. Clin Microbiol
Rev 1999;12(4):564-82.
Darout IA, Christy AA, Skaug N, Egeberg PK. Identification and
quantification of some potentially antimicrobial anionic components in
miswak extract. Indian J Pharmacol 2000;32(1):11-4.
Ibrahim KM, Naem RK, Abd-Sahib AS. Antibacterial activity of
nutmeg (Myristica fragrans) seed extracts against some pathogenic
bacteria. J Al-Nahrain Univ 2013;16(2):188-92.
Takikawa A, Abe K, Yamamoto M, Ishimaru S, Yasui M, Okubo Y,
et al. Antimicrobial activity of nutmeg against Escherichia coli O157.
J Biosci Bioeng 2002;94(4):315-20.
Shafiei Z, Shuhairi NN, Yap NM, Sibungkil CA, Latip J. Antibacterial
activity of Myristica fragrans against oral pathogens. Evid Based
Complement Alternat Med 2012;2012:7.
Published
How to Cite
Issue
Section
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.