ANTIHYPERURICEMIC AND XANTHINE OXIDASE INHIBITORY ACTIVITIES OF FRACTIONS FROM ETHANOLIC LEAVES EXTRACT OF STELECHOCARPUS BURAHOL

Authors

  • Titik Sunarni 1 Bandung Institute of Technology 2 Setia Budi University
  • FRANSISKA LEVIANA
  • IRDA FIDRIANNY
  • MARIA IMMACULATA
  • KOMAR RUSLAN WIRASUTISNA

DOI:

https://doi.org/10.22159/ajpcr.2016.v9i6.14314

Abstract

Objectives: The aims of the research were to evaluate antihyperuricemic and xanthine oxidase (XO) inhibitory activity of fractions from ethanolic leaves extract of Stelechocarpus burahol.

 

Methods: S. burahol leaves powder was extracted in ethanol by maceration method, then extract was fractionated by liquid-liquid extraction method using n-hexane and ethyl acetate. Hyperuricemic rat model was induced by administered potassium oxonate intraperitoneally. The activity of these fractions on XO inhibitory was determined by measuring the uric acid formation using UV-Vis spectrophotometry method after incubating with XO.  

 

Results: The n-hexane and ethyl acetate fractions significantly (p < 0.05) reduce the uric level by 32.0 and 28.0% respectively compared to uric acid level of hyperuricemic group at one hour after drug administration (p < 0.05). On XO inhibitory activity test, only n-hexane fraction exhibited XO inhibitory activity with IC50 541.76 µg/ml.

 

Conclusion: The n-hexane and ethyl acetate fractions from ethanolic leaves extract of S.burahol had antihiperuricemic activity, but less inhibitor effect on XO activity.

 

Keywords: Stelechocarpus burahol, liquid-liquid extraction, antihyperuricemic, xanthine oxidase inhibitory activity 

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Author Biography

Titik Sunarni, 1 Bandung Institute of Technology 2 Setia Budi University

Department of Pharmaceutical Biology

References

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Published

01-11-2016

How to Cite

Sunarni, T., F. . LEVIANA, I. . FIDRIANNY, M. . IMMACULATA, and K. R. . WIRASUTISNA. “ANTIHYPERURICEMIC AND XANTHINE OXIDASE INHIBITORY ACTIVITIES OF FRACTIONS FROM ETHANOLIC LEAVES EXTRACT OF STELECHOCARPUS BURAHOL”. Asian Journal of Pharmaceutical and Clinical Research, vol. 9, no. 6, Nov. 2016, pp. 255-8, doi:10.22159/ajpcr.2016.v9i6.14314.

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