ANTIOXIDANT ACTIVITIES OF VARIOUS SEED EXTRACTS FROM FOUR VARIETIES OF RAMBUTAN (Nephelium lappaceum) USING DPPH AND ABTS ASSAYS
Abstract
Objectives: The objectives of this research were to study antioxidant activities from various seed extracts of four varieties of rambutan using
2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azinobis (3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS) methods and correlation of total flavonoid
content (TFC), total phenolic content (TPC), and total carotenoid content (TCC) with inhibitory concentration 50 (IC
50
) of DPPH and IC
Methods: Antioxidant activities, TFC, TPC, and TCC of various seed extracts were performed by ultraviolet -visible spectrophotometry and their
correlation with IC
50
of DPPH and IC
50
of ABTS antioxidant activities were analyzed by Pearson's method.
Results: Ethanolic seed extract of lebak bulus variety had the lowest IC
of DPPH scavenging activity 7 µg/ml, while ethyl acetate seed extract of rajah
variety had the lowest IC
50
50
of ABTS scavenging activity 7.34 µg/ml. The highest TFC, TPC, and TCC were given by ethyl acetate extract of binjai variety,
ethanolic extract of rapiah variety, and ethanolic extract of rajah variety, respectively. There were negatively high correlation between TFC, TPC,, TCC
in seed extracts of rapiah variety with their IC
50
of DPPH and IC
50
of ABTS scavenging activities.
Conclusions: All of ethyl acetate and ethanolic seed extracts of four varieties of rambutan were very strong antioxidant by DPPH and ABTS methods.
Flavonoid, phenolic, and carotenoid compound in seed extracts of rapiah variety were contributor in their antioxidant activities by DPPH and ABTS
methods. The IC
50
of DPPH and IC
50
of ABTS scavenging activities in seed extracts of lebak bulus, rajah, and rapiah varieties gave linear result.
Keywords: Antioxidant, 2,2-diphenyl-1-picrylhydrazyl, 2,2'-azinobis (3-ethyl-benzothiazoline-6-sulfonic acid, Seed, Four varieties, Rambutan,
Flavonoid, Phenolic, Carotenoid.
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