EXPERIMENTAL EVALUATION OF THE ANTI-ULCER ACTIVITY OF GRAPE (VITIS VINIFERA) SEED EXTRACT IN WISTAR ALBINO RATS AGAINST HYDROCHLORIC ACID – ETHANOL INDUCED ULCER MODEL
DOI:
https://doi.org/10.22159/ajpcr.2016.v9i6.14999Abstract
Background:
Herbal products are finding increasing demand in the treatment of Peptic ulcer, on account of their better safety and efficacy.
Objectives:
To evaluate the anti-ulcer activity of ethanolic extract of seeds of Vitis Vinifera in Wistar albino rats.
Methods:
The ethanolic extract of Vitis Vinifera was investigated for its anti-ulcer activity in rats against HCl – Ethanol induced ulcer model. The antiulcer activity was assessed by determining and comparing gastric volume, pH, free and total acidity; ulcer number and its inhibition, ulcer severity and ulcer index.
Results:
A significant antiulcer activity was observed. The grape seed 100mg/kg group showed significant [p < 0.05] reduction in ulcer number 30.00 ± 3.23 as compared to the control. The grape seed 200mg/kg showed significant [p < 0.05] reduction in ulcer number 27.33 ± 2.97, free acidity 20.00 ± 2.26, and gastric lesion18.00 ± 2.96 as compared to the control.
Conclusion:
This present study indicates that Vitis vinifera seed extract has potential anti ulcer activity in the model tested.
 Keywords: Vitis vinifera, Anti-ulcer, Ethanol, Free acidity, Total acidity
Downloads
References
REFERENCES
Muchandi AA, Chandrashekhar VM. Antiulcer and gastroprotective
potential of Stereospermum suaveolens in wistar rats. J Pharmacol
Pharmacother 2011;2(2):117-9.
Sharma MP, Ahuja V. Peptic ulcer disease and non ulcer dyspepsia.
In: Tandon BN, editor. Tropical Hepato-Gastroenterology. New Delhi:
Elsevier Publishers; 2008. p. 97-115.
Department of Pharmacology and Therapeutics. Herbal medicines –
An evidence based look. Canada: The University of British Columbia;
World Health Organization. Policy Perspectives on Medicines –
Traditional Medicine – Growing Needs and Potential. Vol. 2. Geneva:
World Health Organization; 2002.
Vaughan JG, Geissler CA. The New Oxford Book of Food Plants.
New York: Oxford University Press; 1997.
Ahn HS, Jeon TI, Lee JY, Hwang SG, Lim Y, Park DK. Antioxidative
activity of persimmon and grape seed extract: In vitro and in vivo. Nutr
Res 2002;22(11):1265-73.
Brown JC, Huang G, Haley-Zitlin V, Jiang X. Antibacterial effects
of grape extracts on Helicobacter pylori. Appl Environ Microbiol
;75(3):848-52.
Al-Habib A, Al-Saleh E, Safer AM, Afzal M. Bactericidal effect of
grape seed extract on methicillin-resistant Staphylococcus aureus
(MRSA). J Toxicol Sci 2010;35(3):357-64.
Zayachkivska OS, Konturek SJ, Drozdowicz D, Konturek PC,
Brzozowski T, Ghegotsky MR. Gastroprotective effects of flavonoids
in plant extracts. J Physiol Pharmacol 2005;56 Suppl 1:219-31.
Clifton PM. Effect of grape seed extract and quercetin on cardiovascular
and endothelial parameters in high-risk subjects. J Biomed Biotechnol
;2004(5):272-8.
Maheswari UM, Rao PG. Antihepatotoxic effect of grape seed oil in rat.
Indian J Pharmacol 2004;37(3):179-82.
Sreemantula S, Nammi S, Kolanukonda R, Koppula S, Boini KM.
Adaptogenic and nootropic activities of aqueous extract of Vitis vinifera
(grape seed): An experimental study in rat model. BMC Complement
Altern Med 2005;5:1.
Vitseva O, Varghese S, Chakrabarti S, Folts JD, Freedman JE. Grape
seed and skin extracts inhibit platelet function and release of reactive
oxygen intermediates. J Cardiovasc Pharmacol 2005;46(4):445-51.
Roy AM, Baliga MS, Elmets CA, Katiyar SK. Grape seed
proanthocyanidins induce apoptosis through p53, Bax, and caspase 3
pathways. Neoplasia 2005;7(1):24-36.
Kijima I, Phung S, Hur G, Kwok SL, Chen S. Grape seed extract is an
aromatase inhibitor and a suppressor of aromatase expression. Cancer
Res 2006;66(11):5960-7.
Wen W, Lu J, Zhang K, Chen S. Grape seed extract inhibits angiogenesis
via suppression of the vascular endothelial growth factor receptor
signaling pathway. Cancer Prev Res (Phila) 2008;1(7):554-61.
Kim TH, Jeon EJ, Cheung DY, Kim CW, Kim SS, Park SH, et al.
Gastroprotective effects of grape seed proanthocyanidin extracts
against nonsteroid anti-inflammatory drug-induced gastric injury in
rats. Gut Liver 2013;7(3):282-9.
Hassan HM. Hepatoprotective effect of red grape seed extracts
against ethanol-induced cytotoxicity. Glob J Biotechnol Biochem
;7(2):30-7.
Saito M, Hosoyama H, Ariga T, Kataoka S, Yamaji N. Antiulcer
activity of grape seed extract and procyanidins. J Agric Food Chem
;46(4):1460-4.
Firdous SM, Neraja K, Debnath R, Singha D, Sravanthi K. Evaluation
of antiulcer activity of ethanolic extract of Sechium edule fruits in
experimental rats. Int J Pharm Pharm Sci 2012;4(1):374-7.
Bickel M. Activity on gastrointestinal tract. In: Vogel GH, Vogel WH,
Scholkens BA, Sandow J, Muller G, Vogel WF, editors. Drug Discovery
and Evaluation: Pharmacological Assays. 2nd ed. Germany: Springer
Publications; 2002. p. 867-72.
Ingale AM, Rajendran V, BharatKumar VP. A comparative evaluation
of the anti-ulcer activity of the extracts of seed and skin of Vitis vinifera
(grape) in wistar albino rats. Int J Clin Diagn Res 2014;2(2):I.
Malairajan P, Gopalakrishnan G, Narasimhan S, Veni KJ. Evalution
of anti-ulcer activity of Polyalthia longifolia (Sonn.) Thwaites in
experimental animals. Indian J Pharmacol 2008;40(3):126-8.
Ingale AM, Rajendran V, Kumar VP. A comparative evaluation of the
anti-ulcer activity of the extracts of seed and skin of Vitis vinifera. Int J
Clin Diagn Res 2014;2(2):1.
Mittal R, Bhardwaj RK. Antiulcer agents. In: Gupta SK, editor. Drug
Screening Methods. 2nd ed. New Delhi: Jaypee Brothers Medical
Publishers (P) ltd.; 2009.
Rathnakumar K, Jaikumar S, Sengottuvelu S. Antiulcer activity of
Euphorbia hirta against experimentally induced ulcer in rats. Asian J
Pharm Res 2013;3(3):121-4.
Kumar V, Abbas AK, Fausto N, Aster JC. Robbins and Cotran
Pathological Basis of Diseases. 8th ed. Pennsylvania: Elsevier Saunders
Publishers; 2010.
Shi J, Yu J, Pohorly JE, Kakuda Y. Polyphenolics in grape seedsbiochemistry
and functionality. J Med Food 2003;6(4):291-9.
Ingale AM, Pinnelli VB, Rajendran V. Experimental evaluation of the
anti-ulcer activity of the ethanolic extract of grape (Vitis vinifera) seed
in wistar albino rats against aspirin plus pylorus ligation induced gastric
ulcer model. Int J Basic Clin Pharmacol 2016;5(3):722-7.30. Brzozowski T, Konturek PC, Drozdowicz D, Konturek SJ, ZayachivskaO,
Pajdo R, et al. Grapefruit-seed extract attenuates ethanol-and stressinduced
gastric lesions via activation of prostaglandin, nitric oxide and
sensory nerve pathways. World J Gastroenterol 2005;11(41):6450-8.
Cuevas VM, Calzado YR, Guerra YP, Yera AO, Despaigne SJ,
Ferreiro RM, et al. Effects of grape seed extract, vitamin C, and
vitamin E on ethanol- and aspirin-induced ulcers. Adv Pharmacol Sci
;2011:740687.
Published
How to Cite
Issue
Section
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.
