• Asni Amin Unversity Moslem University
  • Maksum Radji University Indonesia
  • Abdul Mun'im University Indonesia
  • Anton Rahardjo University Indonesia




Objective: This research was conducted to prove the activity of Stelechocarpus burahol fruit (SBF) extracts against volatile sulfur compound (VSC) of methyl mercaptan in anaerobic Gram-negative oral bacteria causing halitosis.

Materials and Methods: Burahol fruits were extracted by ethanol and partitioned by several solvents and then identified the chemical constituents of the extracts using phytochemical screening test and polyphenol content by spectroscopic ultraviolet instrument. The halitosis activity against VSC of methyl mercaptan component derived from anaerobic Gram-negative oral bacteria after administration of SBF extracts with a dose of 20 mg/ml was conducted in vitro using gas chromatography-oral chroma.

Results: All extracts (ethanol, ethyl acetate, butanol, water, and methanol) contain flavonoids and polyphenols, whereas saponin was found in all extracts except methanol. Halitosis activity shows the ethanol extract of SBF, has absorption capability against methyl mercaptan, and was higher than the other extracts, with catechins as control.

Conclusion: All extracts of SBF (ethanol, ethyl acetate, butanol, water, and methanol) can inhibit and reduce VSC of methyl mercaptan causing halitosis.

Keywords: Stelechocarpus burahol, Methyl mercaptan, Halitosis.


Download data is not yet available.

Author Biographies

Asni Amin, Unversity Moslem University

Faculty of Pharmacy

Maksum Radji, University Indonesia

Faculty of Pharmacy

Abdul Mun'im, University Indonesia

Faculty of Pharmacy

Anton Rahardjo, University Indonesia

Faculty of Dentistry


Darusman HS, Rahminiwati M, Sadiah S, Batubara I, Darusman LK, and Mitsunaga T. 2012. Indonesian Kepel fruits (Stelecocarpus burahol) as Oral Deodorant. Res J Med Plant, 6(2): 180-188.

Mun’im, A., 2013, Development of Nutraceuticals : Moringa oleifera Leaves and Stelechocharpus burahol Fruits, 2nd International Conference on Nutraceutical and Cosmetic Science, Jakarta, 32.

Hughesa, Francis J., and McNabb. R., 2008, Oral malodour–A Review. Archives Of Oral Biology, 53, Suppl. 1 S1–S7.

Nachnani, S, 2011, Oral Malodor: Causes, Assessment, and Treatment, Compendium of continuing education in dentistry (Jamesburg, N.J. : 1995) 32 (1): 22–4, 26–8, 30–1; quiz 32, 34. PMID 21462620.

Scully C., and Greenman, J., 2008, Halitosis (Breath Odor), J. Periodontology 2000, Vol. 48, 2008, 66–75

Heyne K. 1987, Useful Plants Indonesia. Forestry Research and Development Agency, Jakarta, Eds.1, translator by Forestry Research and Development Agency, pg. 765

Indonesian Ministry of Health (MOH), 2000, The Standardization Extract Parameters, Jakarta.

Harborne, JB. 1987, Methods Phytochemicals, Guidance How Modern Plant Extract, tlanslator by Padmawinata, Bandung, ITB

Sanz, M., Roldan,S., and Herrera, D., 2001, Fundamentals of Breath Malodour;J Contemp Dent Pract, Nov;2(4):1-17.2(4):1-13.

Negishi O, and Negishi Y. 1999. Enzymatic Deodorization with Raw Fruits, Vegetables and Mushrooms. Food Scie Technol Res.5(2):176-180.

Negishi O, Negishi Y, and Ozawa T, 2000, Enzymatic Deodorization with Variegatic Acid from (Boletus subvelutipes) and Its Mechanism. Food Scie Technol Res, 6(3):186-191

Negishi O, Negishi Y, and Ozawa T. 2002. Effects of Food Materials on Remoral of Allium-Specific Volatile Sulfur Compounds. J Agric Food Chem, 50 (13): 3856-3861

Negishi O, Negishi Y, Aoyagi Y, Sugahara T, and Ozawa T. 2001. Mercaptan-capturing properties of mushrooms. J Agric Food Chem, 49(11): 5509-5514

Negishi O, Negishi Y, Yamaguchi F, and Sugahara T. 2004. Deodorization With Ku-Ding-Cha Containing A Large Amount Of Caffeoyl Quinic Acid Derivatives. Agric Food Chem 2004; 52(17): 5513-5518

Van den Broek, Annemiek M.W.T., Louw Feenstra, Cees de Baat., 2007, Review A Review Of The Current Literature On Aetiology And Measurement Methods Of Halitosis, Journal Of Dentistry 3.



How to Cite

Amin, A., M. Radji, A. Mun’im, A. Rahardjo, and HERMAN SURYADI. “HALITOSIS ACTIVITY AGAINST VSC METHYL MERCAPTAN COMPONENT FROM BURAHOL (Stelechocarpus Burahol) FRUIT EXTRACT”. Asian Journal of Pharmaceutical and Clinical Research, vol. 10, no. 5, May 2017, pp. 116-9, doi:10.22159/ajpcr.2017.v10i5.15862.



Original Article(s)

Most read articles by the same author(s)