PRODUCTION OF KERATINASES FROM NOCARDIOPSIS SP. 28ROR AS A NOVEL IRAQI STRAIN
DOI:
https://doi.org/10.22159/ajpcr.2017.v10i4.16427Abstract
Objectives: isolate a novel feather- degrading actinobacterial species had the ability to produce wide pH activity keratinases.
Methods: Of 23 actinobacterial isolates were recovered from farm soil, poultry farm soil and feather wastes, these isolates were screened for protease and keratinase production on skim milk agar, feather media, and antimicrobial production. One potential isolate was identified depending on phenotypical, physiological and molecular according to partial sequences of 16S r RNA gene analysis and optimized keratinase production.Â
Results:  11 isolates out of 22 protease producer  had the ability to degrade raw chicken feather and some of these  isolates produced antifungal and antibacterial metabolites.The potential isolate,  Nocardiopsis sp. 28ROR (GenBank: KC702802.1), produced two types of extracellular keratinases in feather meal medium at pH6 (acid type), 30-35°C for 7d  and  the alkaline keratinase at pH10, 40°C for 7d.
Conclusion: The Nocardiopsis sp. 28ROR was a novel strain produced keratinases using feather meal degradation as a cheap waste medium. The wide tolerance of temperature and pH by keratinase makes it an ideal contender to be investigated further for potential application as a detergent additive.
Keywords: Nocardiopsis, Keratinase, Optimization, Feather medium, Antibiotic.
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