CYTOTOXIC ACTIVITY OF ALKALOIDS EXTRACTED FROM THREE IRAQI PLANTS AGAINST BREAST CANCER CELL LINE

FARAHA  AL-MARZOOK; Rabab Omran

doi:10.22159/ajpcr.2017.v10i9.19134

Authors

FARAHA AL-MARZOOK Department of Biology, College of Science, University of Babylon, Babylon, Iraq http://orcid.org/0000-0001-9449-3421
Rabab Omran Department of Biology, College of Science, University of Babylon, Babylon, Iraq

DOI:

https://doi.org/10.22159/ajpcr.2017.v10i9.19134

Keywords:

Alkaloids, Eucalyptus camaldulensis Dehnh, Aloe vera L, Capparis spinosa L, In vitro, Cytotoxicity, Breast cancer

Abstract

Â

Â Objectives: Screening for cytotoxic activity of total alkaloid extracts of Eucalyptus camaldulensis, Aloe vera, and Capparis spinosa against breast cancer cell line Michigan Cancer Foundation-7 (MCF-7) and nontumorigenic fetal hepatic cell line (WRL-68).

Methods: The plant powders were extracted separately with 80% methanol and chloroform at pH 2 and 10. Total alkaloids were detected qualitatively by Mayer's, Dragendorff's, and Hagerâ€˜s reagents and estimated quantitatively by bromocresol green spectrophotometry depending on the atropine calibration curve. The cytotoxic activity was evaluated by 3-[4, 5-dimethylthiazoyl]-2, 5-diphenyltetrazolium bromide assay.

Results: The extract of E. camaldulensis had highest total alkaloid content (24.50Â±1.70 mg/100 g plant dry weight) than the others. The total alkaloids (400 Î¼g/ml) of E. camaldulensis reduced the cell viability of both cell lines MCF-7 and WRL-68 to 45.25Â±2.20% and 92.00Â±1.55%, respectively, and the inhibitory concentration 50% of cells were 375.50 Î¼g/ml for MCF-7. The alkaloids of C. spinosa had effect 79.80Â±7.08% and 89.50Â±0.09% against MCF-7and WRL-68, respectively. While the total alkaloids of A. vera had slightly effect on both cell lines.

Conclusion: Plant alkaloids appeared variable cytotoxic activity against cancer and normal cell lines depending on the alkaloid contents, concentrations, purity, and cell line types.

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Author Biography

FARAHA AL-MARZOOK, Department of Biology, College of Science, University of Babylon, Babylon, Iraq

biology deparament , biotechnology-genetic engineering

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