KAEMPFEROL FROM STELECHOCARPUS BURAHOL, (BL.) HOOK F. & TH. LEAVES AND XANTHINE OXIDASE INHIBITION ACTIVTY

DINIATIK; SUWIJIYO  PRAMONO; Sugeng Riyanto

doi:10.22159/ajpcr.2017.v10i4.16792

Authors

DINIATIK Universitas Muhammadiyah Purwokerto
SUWIJIYO PRAMONO
Sugeng Riyanto

DOI:

https://doi.org/10.22159/ajpcr.2017.v10i4.16792

Abstract

Objective: Stelechocarpus burahol, (Bl.) Hook f. & Th. is a plant widely distributed in Java Island of Indonesia. The aim of this study is to identify compounds from the leaves of S. burahol that exhibited activity as xanthine oxidase inhibitor.

Methods: The leaves were extracted with aqueous ethanolÂ and hidrolized with HCl methanol, then partitioned sequentially with chloroform and ethyl acetate. The ethyl acetate fractions were separated by coloumn chromatography with cellulose as stationary phase and methanol 50% as mobile phase.

Results: Purification from this extracts afforded three compounds with one compound identified, namely Kaempferol. The four compounds possessed as xanthine oxidase inhibitor with IC₅₀ values ranging from 0.27 to 0.45 Î¼g/ml.

Conclusion:Kaempferol exhibited the highest inhibition of 0.27 Î¼g/ml.

Keywords: Kaempferol, Xanthine Oxidase Inhibitor, Stelechocarpus burahol, (Bl.) Hook f. & Th.

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References

Indonesian Spice and Medicinal Crops Research Institute (ISMCRI). Stelechocarpus burahol - Rare Medicinal Plants (Report). Indonesia: ISMCRI Bogor; 2013.

Suparmi, Isradji I, Yusuf I, Fatmawati S, Ratnaningrum IH, Fuadiyah S, et al. Anti-implantation activity of kepel (Stelechocarpus burahol) pulp ethanol extract in female mice. J Pure Appl Chem Res 2015;4(3):94-9.

Verheij EW, Caronel RE. Sumber Daya Nabati Asia Tenggara. Jakarta: Gramedia; 1997.

Purwantiningsih, Purwantini I, Santoso D. Identification of standars parameters of kepel leaves (Stelechocarpus burahol (bl.) hook f. & Th.) and the extract as raw material for anti-hyperuricemic medicaments. Asian J Pharm Clin Res 2011;4(1):149-53.

Darusman HS. Pharmacology Activity of Kepel Plant (Stelechocarpus burahol (Blume) Hook & Thompson) as Topical and Oral Deodorant (Thesis). Indonesia: Veterinary Faculty, Bogor Agricultural Institute; 2010.

Tisnadjaja D, Saliman E, Silvia, Simanjuntak P. Study of Stelechocarpus burahol (bl.) Hook f. & Th. As an antioxidative compound containing fruit. Biodiversitas 2006;7(2):199-202.

Susilowati I. Activity study of Stelechocarpus burahol (bl.) Hook f. & Th. Leaves to Eliminate Uric Acid Content (Skripsi). Indonesia: Ahmad Dahlan University; 2000.

Hening TH. Effect of Infusa from Stelechocarpus burahol. (bl) Hook f. & Th. Leaves to Serum Uric Acid Level on Liver Induced Chicken (Skripsi). Indonesia: Sanata Dharma University; 2002.

Sunarni T, Pramono S, Asmah R. Antioxidant-free radical scavenging of flavonoid from the leaves of Stelechocarpus burahol (bl.) Hook f. & Th. Leaves on the body weight ad organ of sparague-dawley rats. Indones J Pharm 2007;18(3):111-6.

Diniatik, Pramono S, Riyanto S. Determination of total flavonoid content of ethyl acetat and chloroform fraction of hydrolysis result from ethanolic extract of Stelechocarpus burahol (bl.) Hook f. & Th. Leaves by using spectrophotometry method. Kartika J Ilmiah Farmasi 2015;3(1):54-60.

Nuki G, Simkin PA. A concise history of gout and hyperuricemia and their treatment. Arthritis Res Ther 2006;8 Suppl 1:S1.

Ernawati, Susanti H. In vitro xanthine oxidase inhibition activity of the sarang semut (Myrmecodia tuberosa (non Jack) Bl.) ethanol extract. PharmaÃ§iana 2014;4(1):15-22.

Anonymous. Enzymatic Assay of Xanthine Oxidase (EC 1.1.3.22). Mississauga, Ontario, Canada: Sigma Aldrich; 1994.14. Nagao A, Seki M, Kobayashi H. Inhibition of xanthine oxidase by flavonoids. Biosci Biotechnol Biochem 1999;63(10):1787-90.

Van Hoorn DE, Nijveldt RJ, Van Leeuwen PA, Hofman Z, Mâ€™Rabet L, De Bont DB, et al. Accurate prediction of xanthine oxidase inhibition based on the structure of flavonoids. Eur J Pharmacol 2002;451(2):111-8.

Purwantiningsih, Hakim AR, Purwatini I. Antihyperuricemic activity of the kepel (Stelechocarpus burahol (Bl.) Hook f. & Th.) leaves extract and xantine oksidase inhibitory study. Int J Pharm Pharm Sci 2010;2(2):122-7.

Haddi R, Marouf A. Xanthine oxidase inhibitory effects of Pistacia lentiscus L. Leaves extracts. Int J Pharm Pharm Sci 2015;7(2):34-9.

Septiningsih U, Susanti H, Widyaningsih W. Xanthine oxidase inhibitory of ethanolic extract of sambiloto root. PharmaÃ§iana 2012;2(2):153-63.

Sikorka M, Matlawska I. Kaempferol, isorhamnetin and their glycoside in the flowers of Asclepias syriaca L. Acta Pol Pharm 2001;58(4):269-72.

Mabry TJ, Markham KR, Thomas MB. The Systematic and Identification of Flavonoid. New York: Springer-Verlag; 1970.

Supratman U. Struktur Elusidasi Organic Compound. 1st ed. Indonesia: Widya Padjajaran; 2010.

Socrates G. Infrared Characteristic Group Frequencies Tables and Charts. New York: John Willey and Sons; 1994.

Stuart BH. Infrared Spectroscopy: Fundamentals and Applications. Chichester, UK: Wiley; 2004.

Anderson OM, Markham KR. Flavonoids Chemistry, Biochemistry and Applications. London: Taylor and Francis Group; 2006.

Mathias L, Vieira JC, Filho RB, Filho ER. A new pentacyclic triterpene isolate from Myroxylon balsamum (syn. Myroxylon peruiferum). J Braz Chem Soc 2000;11(2):195-8.

Kemp, W. Organic Spectroscopy. 3rd Ed. London: Macmillan; 1993.

Lam LH, Sakaguchi K, Ukeda H, Sawamura M. Flow injection determination of xanthine oxidase inhibitory activity and its application to food samples. Anal Sci 2006;22(1):105-9.

Massey V, Komai H, Palmer G, Elion GB. On the mechanism of inactivation of xanthine oxidase by allopurinol and other pyrazolo[3,4-d]pyrimidines. J Biol Chem 1970;245(11):2837-44.

Kumar R, Darpan, Sharma S, Singh R. Xanthine oxidase inhibitors: A patent survey. Expert Opin Ther Pat 2011;21(7):1071-108.