ANTIOXIDANT ACTIVITY OF RED DRAGON FRUIT PEEL (HYLOCEREUS POLYRHIZUS (F.A.C. WEBER) BRITTON AND ROSE) ISOLATES USING 2,2-DIPHENYL-1-PICRYLHYDRAZYL METHOD
Keywords:Red dragon fruit peel, Isolate, Antioxidant, 2, 2-diphenyl-1-picrylhydrazyl
Â Objective: Indonesia is a country which has various natural resources including medicinal plants. Among the plants, red dragon fruit is interesting to be studied. In this study, antioxidant activity of red dragon fruit peel (Hylocereus polyrhizus (F.A.C. Weber) Britton and Rose) isolate was tested using 2,2-diphenyl-1-picrylhydrazyl method.
Methods: Isolation of active compounds was performed through the application of vacuum liquid chromatography and preparative thin-layer chromatography (TLC). Antioxidant activity was tested using both TLC and spectrophotometry.
Result: Results revealed that the values of IC50 of isolate 1 and 2 were 2.952, 14 Î¼g/mL and 25.635,95 Î¼g/mL, respectively.
Conclusion: Isolate of dragon fruit has antioxidant activity. The compound which results in antioxidant activity is terpenoid and steroid.
Omidizadeh A, Yusof RM, Ismail A, Roohinejad S, Nateghi L, Bakar MZ. Cardioprotective compounds of red pitaya (Hylocereus lemairei) fruit. J Food Agric Environ 2011;9:152-6.
Jamilah B, Shu CE, Kharidah M, Dzulkifly MA, Noranizan A. Physico-chemical characteristics of red pitaya (Hylocereus lemairei) peel. Int Food Res J 2011;18:279-86.
Wu L, Hsu HW, Chen YC, Chiu CC, Lin YI, Ho JA. Antioxidant and antiproliferative activities of red pitaya. Food Chem 2006;95:319-27.
Nurliyana R, Syed ZI, Mustapha SK, Aisyah MR, Kamarul RK. Antioxidant study of pulp and peel dragon fruits: A comparative study. Int Food Res J 2010;17:365-75.
Wahdaningsih S, Wahyuono S, Riyanto S, Murwanti R. The radical scavenging activity of 2-2â€™ diphenyl-1- picrylhydrazil (dpph) on the methanol extracts and ethyl acetate fractions of red dragon fruit peel (Hylocereus polyrhizus (F.A.C.Weber) Britton dan Rose). Int J Phytomed 2017;9:79-82.
Pham-Huy LA, He H, Pham-Huy C. Free radicals, antioxidants in disease and health. Int J Biomed Sci 2008;4:89-96.
Sunarni T, Pramono S, Asmah R. Antioxidant free radical scavenging of flavonoid from the leaves of Stelechocarpus burahol (B1. Hook f. and Th). leaves on the body weight and organ of sparague-dawley rats. Indones J Pharm 2007;18:111-6.
Thenmozhi K, Karthika K, Jamuna S, Paulsamy S, Manian S, Chitravadivu C. In vitro antioxidant and radical scavenging abilities of aqueous methanolic extract of Cassia obtosa L. plant parts (Caesalpiniaceae). Int J Pharm Pharm Sci 2015;7:340-4.
Mohammad SN, Srinivasulu A, Chitibabu B, Rao MU. Isolation and purification of antibacterial principle from Avicennia marina L in methanol. Int J Pharm Pharm Sci 2015;7:38-41.
Kikuzaki H, Hisamoto M, Hirose K, Akiyama K, Taniguchi H. Antioxidant properties of ferulic acid and its related compounds. J Agric Food Chem 2002;50:2161-8.
Demirezer LO, KuruÃ¼zÃ¼m-Uz A, Bergere I, Schiewe HJ, Zeeck A. The structures of antioxidant and cytotoxic agents from natural source: anthraquinones and tannins from roots of Rumex patientia. Phytochemistry 2001;58:1213-7.
Molyneux P. The use of the stable free radical diphenyl picrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J Sci Technol 2004;26:211-9.
How to Cite
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.