EVALUATION OF ANTIBACTERIAL ACTIVITY OF ESSENTIAL OIL OF CINNAMOMUM ZEYLANICUM, EUGENIA CARYOPHYLLATA, AND ROSMARINUS OFFICINALIS AGAINST STREPTOCOCCUS ORALIS

Samira Hsaine; Reda Charof; Khadija Ounine

doi:10.22159/ajpcr.2017.v10i5.17072

Authors

Samira Hsaine Laboratory of Biology, Health and Environment, Applied Microbiology Team, Faculty of Sciences, Ibn Tofail University, Kenitra, Morocco http://orcid.org/0000-0002-6996-5818
Reda Charof National Institute of Hygiene, , Rabat Morocco.
Khadija Ounine Laboratory of Biology, Health and Environment, Applied Microbiology Team, Faculty of Sciences, Ibn Tofail University, Kenitra, Morocco

DOI:

https://doi.org/10.22159/ajpcr.2017.v10i5.17072

Keywords:

Essential oil, Cinnamomum zeylanicum, Eugenia caryophyllata, Rosmarinus officinalis, Antimicrobial activity, Streptococcus oralis

Abstract

Objective: Streptococcus oralis plays an important role in the biofilm formation of dental plaque and the occurrence of periodontal disease. The
present study was conducted to evaluate in vitro antibacterial activity of three essential oils, namely, Cinnamomum zeylanicum, Eugenia caryophyllata,
and Rosmarinus officinalis against S. oralis.

Methods: The antibacterial activity of essential oils was investigated by diffusion method using sterile discs (or aromatograms). The minimum
inhibitory concentration (MIC) of essential oils showing important antibacterial activity was measured using the broth dilution method.

Results: Evaluation of the antibacterial activity of three essential oils as determined by the aromatogram technique showed that the essential oil
of R. officinalis had no effect on S. oralis, while the latter was extremely sensitive to the other two essential oils, but with a higher efficiency of the
essential oil of C. zeylanicum (42 mm diameter) than E. caryophyllata (20 mm diameter). Similarly, the MIC and minimum bactericidal concentration
(MBC) were higher for the essential oil of C. zeylanicum than the essential oil of E. caryophyllata. The MBC/MIC ratio is of the order of 2. The essential
oils studied therefore appear to exert bactericidal activity against S. oralis.

Conclusion: The findings suggest that essential oils of C. zeylanicum and E. caryophyllata may be used as an alternative to synthetic antibiotics.

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Author Biographies

Samira Hsaine, Laboratory of Biology, Health and Environment, Applied Microbiology Team, Faculty of Sciences, Ibn Tofail University, Kenitra, Morocco

Department of Biology, PhD Student

Reda Charof, National Institute of Hygiene, , Rabat Morocco.

Department of Medical Bacteriology,

Medecin Biologiste

Khadija Ounine, Laboratory of Biology, Health and Environment, Applied Microbiology Team, Faculty of Sciences, Ibn Tofail University, Kenitra, Morocco

Department of Biology

Professor of Higher Education

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