EVALUATION OF ANTI-DIABETIC ACTIVITY OF HYDNOCARPUS LAURIFOLIA IN STREPTOZOTOCIN INDUCED DIABETIC RATS.
Abstract
Objective: The objective of this study was to evaluate the anti-hyperglycemic activity of different extracts of Hydnocarpus laurifolia seeds in both normal and diabetic rats.
Methods: Male Wistar rats weighing about 180-250 g were taken and divided into eleven groups, with six rats in each group. Diabetes was induced by giving streptozotocin (30-50 mg/kg) intraperitoneally. Rats that showed blood glucose levels >250 mg/dl were selected for the study. Metformin (50 mg/kg) was given as a standard oral hypoglycemic agent. Oral glucose tolerance test was performed in all groups of rats.
Results: The petroleum ether and ethylacetate extracts of H. laurifolia seeds at different doses was prepared and administered orally. The blood glucose levels were estimated by glucose-oxidase method. Anti-hyperglycemic activity of the test drugs in diabetic rats showed a significant reduction in blood glucose levels (p<0.0001) at 1 hr, 2 hr and 4 hrs respectively when compared to diabetic group.
Conclusion: The results suggested that H. laurifolia seed extract may have potent anti-diabetic activity, justifying the use of the drug for the treatment of diabetes mellitus.
Keywords: Hydnocarpus laurifolia, Achariaceae, Oral glucose tolerance test, Anti-hyperglycemic activity
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