ASSESMENT OF ANTI-INFLAMMATORY ACTIVITY AND CYTOTOXICITY OF FREEZE DRIED HYDROALCOHOLIC EXTRACT OF Bidens andicola ON ISOLATED NEUTROPHILS

Vinueza D; LÃ“pez E; Acosta K; Abdo S

doi:10.22159/ajpcr.2017.v10i6.17574

Authors

Vinueza D Natural Products Laboratory, Science Faculty, Polytechnic School of Chimborazo, Panamericana Sur km 1½, CP 060155, Riobamba, http://orcid.org/0000-0002-6910-0726
LÃ“pez E Natural Products Laboratory, Science Faculty, Polytechnic School of Chimborazo, Panamericana Sur km 1½, CP 060155, Riobamba,
Acosta K Natural Products Laboratory, Science Faculty, Polytechnic School of Chimborazo, Panamericana Sur km 1½, CP 060155, Riobamba,
Abdo S Natural Products Laboratory, Science Faculty, Polytechnic School of Chimborazo, Panamericana Sur km 1½, CP 060155, Riobamba,

DOI:

https://doi.org/10.22159/ajpcr.2017.v10i6.17574

Keywords:

Anti-inflammatory, Cytotoxicity, Bidens andicola, Cell proliferation reagent, Water-soluble tetrazolium salts

Abstract

Objective: The aim of this study was to evaluate anti-inflammatory activity and cytotoxicity in vitro of hydroalcoholic extract of Bidens andicola.

Methods: B. andicola hydroalcoholic extract was obtained from aerial parts of B. andicola, following a standardized methodology. Briefly, aerial parts of B. andicola were extracted with ethanol 70% v/v and defatted with n-hexane, hydroalcoholic fraction was concentrated under controlled conditions in a rotary evaporator, and finally the residue was freeze-drying to obtain the hydroalcoholic extract of B. andicola. Anti-inflammatory activity and cytotoxicity assays were carried out using in vitro isolated neutrophils model using stable water-soluble tetrazolium salts.

Results and Conclusions: The in vitro anti-inflammatory assay on isolated neutrophils demonstrated that the hydroalcoholic extract showed antiinflammatory
activity compared to aspirin, with inflammatory inhibition percent values of 80.138Â±0.729 to hydroalcoholic extract of B. andicola and 82.117Â±0.762 to aspirin, each tested in five replicates at the concentration of 200 ppm of hydroalcoholic extract or reference.

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Author Biography

Vinueza D, Natural Products Laboratory, Science Faculty, Polytechnic School of Chimborazo, Panamericana Sur km 1½, CP 060155, Riobamba,

Grupo de InvestigaciÃ³n de Productos Naturales y Farmacia - Professor Researcher.

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