OREOCALLIS GRANDIFLORA PHOTOPROTECTIVE EFFECT AGAINST ULTRAVIOLET B RADIATION-INDUCED CELL DEATH

Vinueza D; Cajamarca D; Acosta K; Pilco G

doi:10.22159/ajpcr.2018.v11i2.20910

Authors

Vinueza D Natural Products Laboratory, Science Faculty, Polytechnic School of Chimborazo, CP 060155, Riobamba, Ecuador. http://orcid.org/0000-0002-6910-0726
Cajamarca D Natural Products Laboratory, Science Faculty, Polytechnic School of Chimborazo, CP 060155, Riobamba, Ecuador.
Acosta K Natural Products Laboratory, Science Faculty, Polytechnic School of Chimborazo, CP 060155, Riobamba, Ecuador.
Pilco G Natural Products Laboratory, Science Faculty, Polytechnic School of Chimborazo, CP 060155, Riobamba, Ecuador.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i2.20910

Keywords:

Photoprotective, Ultraviolet-B, Oreocallis grandiflora, Ultraviolet-B-induced cell death, Escherichia coli

Abstract

Objective: The aim of this research was to evaluate the photoprotective effect of Oreocallis grandiflora hydroalcoholic extract (OGHE) against
ultraviolet (UV)B-induced cell death model on a strain of Escherichia coli (ATCC 25922) and to determine the sun protection factor (SPF) using the
equation proposed by Mansur.
Methods: OGHE was obtained from leaves of O. grandiflora, following a standardized methodology. In short, O. grandiflora leaves were extracted with
ethanol 70% v/v and defatted with n-hexane, hydroalcoholic fraction was concentrated under controlled conditions through rotary evaporator, and
finally, the residue was freeze drying to obtain OGHE. The photoprotective effect was carried out using in vitro UVB-induced cell death model on a
strain of E. coli (ATCC 25922), like a first approach to study its potential application on cosmetics.
Results and Conclusions: From results, O. grandiflora is an important resource to produce new cosmetic products. However, the safety of OGHE is
necessary to a rational development in that sense. OGHE shows advantages in relation to conventional active compounds of commercial sunscreens used
in this research (2-ethylhexyl 4-methoxycinnamate and 2-ethylhexyl 4-(dimethylamino)benzoate) at the concentration of 2 mg/mL, on survivor time
(with OGHE until 120 min), range of inactivation of E. coli caused by UVB (OGHE K value minor against to positive controls), and high SPF (13.56Â±0.21).

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Author Biography

Vinueza D, Natural Products Laboratory, Science Faculty, Polytechnic School of Chimborazo, CP 060155, Riobamba, Ecuador.

Grupo de InvestigaciÃ³n de Productos Naturales y Farmacia - Professor Researcher.

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