MYCOSYNTHESIS OF SILVER NANOPARTICLES: CHARACTERIZATION, ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITY FROM PLEUROTUS FLORIDA (MONT) SINGER: A MACRO FUNGI
DOI:
https://doi.org/10.22159/ajpcr.2017.v10i9.19090Keywords:
Pleurotus florida, Mycosynthesis of silver nanoparticle, Anti-inflammatory, AntioxidanAbstract
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 Objective: To determine the antioxidant and anti-inflammatory activity of myco-synthesized silver nanoparticle (AgNP) from Pleurotus florida.
Methods: Fresh mushrooms were collected, processed, and the AgNPs were synthesized using standard reducing agent 1 mM of silver nitrate. The characteristics of synthesized particles were confirmed using ultraviolet, Fourier transmission infrared, X-ray diffraction, and energy dispersive X-ray analysis. These nanoparticles were subjected for antioxidant activity through 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay and anti-inflammatory activity using bovine serum albumin denaturation assay, respectively, and the percentage of inhibition of AgNPs was calculated with standard error mean.
Results: The AgNPs were recorded the absorbance band at 410 nm was the particle size of 20-50 nm with a rod in shape. In DPPH assay the antioxidant activity was recorded as 96.2% with 50% inhibitory concentration (IC50) value of 85 μg/ml. In anti-inflammatory activity, the activity was recorded as 92.10±0.005% and IC50 value was recorded as 100 μg/ml.
Conclusion: The results indicated the significant antioxidant and anti-inflammatory activity of AgNPs from P. florida. Hence, further in vivo studies are focused to confirm the anticancer and anti-inflammatory activities.
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