ANTIDIABETIC ACTIVITY OF EXTRACT, FRACTIONS, AND ASIATICOSIDE COMPOUND ISOLATED FROM CENTELLA ASIATICA LINN. LEAVES IN ALLOXAN-INDUCED DIABETIC MICE

Authors

  • Eka Fitrianda Department of Pharmacy, Pharmacology-Clinical Pharmacy Research Group, School of Pharmacy, Institut Teknologi Bandung, Bandung, Indonesia.
  • Elin Yulinah Sukandar Department of Pharmacy, Pharmacology-Clinical Pharmacy Research Group, School of Pharmacy, Institut Teknologi Bandung, Bandung, Indonesia.
  • Elfahmi Elfahmi Department of Pharmacy, Pharmacology-Clinical Pharmacy Research Group, School of Pharmacy, Institut Teknologi Bandung, Bandung, Indonesia.
  • I Ketut Adnyana Department of Pharmacy, Pharmacology-Clinical Pharmacy Research Group, School of Pharmacy, Institut Teknologi Bandung, Bandung, Indonesia.

DOI:

https://doi.org/10.22159/ajpcr.2017.v10i10.20419

Keywords:

Centella asiatica, Fraction, Asiaticoside, Diabetes mellitus, Alloxan

Abstract

 

 Objective: Centella asiatica Linn. is known and used as traditional antidiabetic drug especially in ayurvedic traditional system in some countries. The main objective of this study was to evaluate antidiabetic activity of ethanolic extract, saponin-rich fraction (SRF), nonsaponin fraction (NSF), and asiaticoside compound isolated from C. asiatica Linn. leaves in alloxan-induced diabetic mice.

Methods: Diabetes was induced in albino mice by administration of alloxan monohydrate (60 mg/kg i.v). Extract was administrated orally each in doses of 125, 250 and 500 mg/kg, the SRF and NSF were in doses of 75, 150 and 300 mg/kg, while the asiaticoside was administrated in doses of 1.4, 2.8 and 5.6 mg/kg for 21 days. Blood fasting glucose level, insulin level, and glycosylated hemoglobin level were measured, and histopathology of pancreas was observed to determine antidiabetic activity of each sample.

Results: Decreased blood fasting glucose level, increased insulin, and decreased glycosylated hemoglobin were observed in diabetic mice treated with extract, SRF and asiaticoside. On 21st day, extract at dose 500 mg/kg, SRF at dose 300 mg/kg, and asiaticoside at dose 5.6 mg/kg gave lowest blood glucose level in their each group which were 239±8.0, 254±10.4, and 217±8.1 mg/dl, respectively, compared to control group at 290±14.8 mg/dl. Insulin level of these three groups was 19.4±0.59, 24.8±0.99, and 27.8±0.77 μIU/ml, respectively, the highest value in their each group compared to 7.28±0.34 μIU/ml in control group. Meanwhile glycated hemoglobin level of these groups was 2.040±0.08, 2.010±0.04, and 2.11±0.07 ng/ml, respectively, lowest value in their each group compared to control group at 2.76±0.06 ng/ml. However, histopathology study in these groups did not show any improvements in regeneration of β-cells of pancreas. In the other side, NSF treatment did not affect any the parameters mentioned above.

Conclusions: It can be concluded that extract, SRF and asiaticoside isolated from C. asiatica Linn. possess significant antidiabetic activity in diabetic mice. Based on increased levels of insulin and histopathology studies of pancreatic tissue, antidiabetic activity of those samples was believed to occur through the mechanism of increasing secretion of healthy pancreatic β-cells without any ability to regenerate β-cells that were damaged by alloxan.

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References

Wild S, Roglic G, Green A, Sicree R, King H. Global prevalence of diabetes mellitus, estimates for the year 2000 and projections for 2030. Diabetes Care 2004;27(5):1047-53.

Rahman MM, Sayeed MS, Haque MA, Hassan MM, Islam SM. Phytochemical screening, antioxidant, anti-Alzheimer and anti-diabetic activities of Centella asiatica. J Nat Prod Plant Resour 2012;2(4):504-11.

Swargiary A, Hankhray B, Brahma BK, Rahman S. Ethno-botanical study of anti-diabetic medicinal plants used by the local people of Kokrajhar district of Bodoland territorial council India. J Med Plants Stud 2013;1:51-8.

Radhika S, Senthilkumar R, Arumugam P. A review on ethnic florae with anti-hyperglycemic efficacy. Int J Herb Med 2013;1(4):55-62.

de Padua LS, Bunyapraphatsara N, Lemmens RH. Plant Resources of South-East Asia. 2nd ed. Leiden: Backhuy Publisher; 1999.

Tiwari S, Gehlot S, Gambhir IS. Centella asiatica: A concise drug review with probable clinical uses. J Stress Physiol Biochem 2011;7(1):38-44.

Seevaratnam V, Banumathi P, Premalatha MR, Sundaram SP, Arumugam T. Functional properties of Centella asiatica (L): A review. Int J Pharm Pharm Sci 2012;4(5):8-14.

Zheng CJ, Qin LP. Chemical components of Centella asiatica and their bioactives. J Chin Integr Med 2007;5(3):348-51.

Shirwaikar A, Rajendran K, Barik R. Effect of aqueous bark extract of Garuga pinnata Roxb. In streptozotocin-nicotinamide induced Type-II diabetes mellitus. J Ethnopharmacol 2006;107(2):285-90.

Kannabiran K, Kuma M, Gunasekar V. Evaluation of antimicrobial activity of saponin isolated from Solanum xanthocarpum and Centella asiatica. Int J Nat Eng Sci 2009;3:25-28.

Fröde TS, Medeiros YS. Animal models to test drugs with potential anti-diabetic activity. J Ethnopharmacol 2008;115(2):173-83.

Ramya SS, Vijayanand N, Rathinavel S. Antidiabetic activity of Cynodon dactylon (L.) Pers. Extracts in alloxan induced rats. Int J Pharm Pharm Sci 2014;6:348-52.

Tanquilut NC, Tanquilut MR, Estacio MA, Torres EB, Rosario JC, Reyes BA. Hypoglycemic effect of Lagerstroemia speciosa (L.) Pers. On alloxan-induced diabetic mice. J Med Plants Res 2009;3(12):1066-71.

Sruthi T, Satyavati D, Upendar K, Kumar CP. Antidiabetic activity and anti-oxidant activity of niddwin, a polyherbal formulation in alloxan induced diabetic rats. Int J Pharm Pharm Sci 2014;6(2):273-7.

Szudelski T. The mechanism of alloxan and streptozotocin action in cells of the rat pancreas. Physiol Res 2011;50(6):536-46.

Federiuk IF, Casey HM, Quinn MJ, Wood MD, Ward WK. Induction of Type-1 diabetes mellitus in laboratory rats by use of alloxan: Route of administration, pitfalls, and insulin treatment. Comp Med 2004;54(3):252-7.

Machado AF, Zimmerman EF, Hovland DN Jr, Weiss R, Collins MD. Diabetic embryopathy in C57BL/6J mice. Altered fetal sex ratio and impact of the splotch allele. Diabetes 2001;50(5):1193-9.

Chauhan PK, Pandey IP, Dhatawalia VK, Singh V. Anti-diabetic effect of ethanolic and methanolic leaves extract of Centella asiatica on allloxan induced diabetic rats. Int J Pharm Bio Sci 2010;1:1-6.

Gayathri V, Leksmi P, Padmadabbhan RN. Anti-diabetes mellitus activity of ethanol extract of Centella asiatica (L.) Urban (whole plant) in streptozotocin-induced diabetic rats, isolation of an active fractions and toxicity evaluation of the extract. Int J Med Aromat Plants 2011;1:278-86.

Alfarra HY, Omar MN. Centella asiatica: From folk remedy to the medicinal biotechnology - A state revision. Int J Biosci 2013;3(6):49-67.

d’Emden M. Glycated Haemoglobin for the Diagnosis of Diabetes. Australia: Australian Prescriber; 2014.

Stratton IM, Adler AI, Neil HA, Matthews DR, Manley SE, Cull CA, et al. Association of glycaemia with macrovascular and microvascular complications of Type 2 diabetes (UKPDS 35): Prospective observational study. BMJ 2000;321(7258):405-12.

Khaw KT, Wareham N, Bingham S, Luben R, Welch A, Day N. Association of hemoglobin A1c with cardiovascular disease and mortality in adults: The European prospective investigation into cancer in Norfolk. Ann Intern Med 2004;141(6):413-20.

Selvin E, Steffes MW, Zhu H, Matsushita K, Wagenknecht L, Pankow J, et al. Glycated hemoglobin, diabetes, and cardiovascular risk in nondiabetic adults. N Engl J Med 2010;362(9):800-11.

Published

01-10-2017

How to Cite

Fitrianda, E., E. Y. Sukandar, E. Elfahmi, and I. K. Adnyana. “ANTIDIABETIC ACTIVITY OF EXTRACT, FRACTIONS, AND ASIATICOSIDE COMPOUND ISOLATED FROM CENTELLA ASIATICA LINN. LEAVES IN ALLOXAN-INDUCED DIABETIC MICE”. Asian Journal of Pharmaceutical and Clinical Research, vol. 10, no. 10, Oct. 2017, pp. 268-72, doi:10.22159/ajpcr.2017.v10i10.20419.

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