THE INVESTIGATION OF CONDITIONS OF API FROM GROUP OF CALCIUM CHANNEL BLOCKERS EXTRACTION BY ORGANIC SOLVENTS BY USING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY AS ASSAY METHOD
Keywords:Extraction, Organic solvents, Amlodipine, Nifedipine, Verapamil, High-performance liquid chromatography
Â Objective: The objective of this research was to select the optimal conditions for extraction of API from calcium channel blockers (amlodipine, nifedipine, and verapamil) by organic solvents from water solutions in dependence on pH solutions.
Methods: The chromatographic analysis of amlodipine, nifedipine, and verapamil performed on liquid chromatography Agilent 1200.
Results: The quantitative determination of API from calcium channel blockers (amlodipine, nifedipine, and verapamil) by high-performance liquid chromatography - method has been conducted. As a result of studies, we have found that the optimal extragent is chloroform, which is extracted at pH 7-82.1% of amlodipine, pH 5-76.2% of nifedipine, and pH 8-95.1% verapamil hydrochloride. API from calcium channel blockers (amlodipine, nifedipine, and verapamil) least extracted with hexane at pH 2.0, so these conditions may be cleaned extract from coextractives impurities.
Conclusion: The results obtained in this research work clearly indicated that the extraction of API from calcium channel blockers (amlodipine, nifedipine, and verapamil) by organic solvents from water solutions in dependence on pH solutions has been conducted.
Clarke EJ. Isolation and Identification of Drugs in Pharmaceuticals, Body Fluids and Postmortem Material. London: Pharmaceutical Press, Electronic Version; 2005.
United States Pharmacopoeia (USP). Medicare Prescription Drug Benefit Model Guidelines Source Information. Available from: http://www.nlm.nih.gov.
State Pharmacopoeia of Ukraine. In: State Enterprise Ukrainian Scientific Center Pharmacopoeia Quality Medicines. 2nd ed., Vol. 2, 3. Kharkiv: State Enterprise Ukrainian Scientific Center of Quality Pharmacopoeia of Medicines; 2014. p. 724.
State Pharmacopoeia of Ukraine State Enterprise Scientific and Expert Centre Pharmacopoeia, Appendix 2. 1st ed. Kharkiv: State Enterprise Ukrainian Scientific Center of Quality Pharmacopoeia of Medicines; 2008. p. 620.
Logoyda L. Development and Validation of New methods of analysis for the determination of different natural and synthetic original active pharmaceutical ingredients in medicines. Duphat (Dubai International Pharmaceutical and Technologies Conference and Exhibition, UAE); 2015. p. 48.
Liliya L, Dmutro K, Stanislav S. Development of methods for identification of calcium channel blockers in medicines. Int J Res Ayurveda Pharm 2016;7(2):88-91.
Logoyda L, Korobko D, Ivanusa I, Serhii K. Development of the methodology of the chromatographic determination of nifedipine in medicines. Asian J Pharm Clin Res 2017;10(3):149-52.
Kondratova Y, Adebayo T, Logoyda L, Korobko D, Berdey I, Kuchmerovska T. Development of the methodology of the chromatographic determination of amlodipine in medicines. Int J Res Ayurveda Pharm 2016;7(4):32-5.
Saifi M, Ali1 A, Saini M, Nasrullah N, Khan S, Abdin MZ. A rapid and efficient high-performance thin-layer chromatographic (HPTLC) method for simultaneous analysis of stevioside and rebaudioside-a in stevia rebaudiana. Int J Pharm Pharm Sci 2014;6(5):455-64.
Sharma T, Moitra SK, Si SC, Sankar DG. Development and validation of a HPLC method for the determination of valsartan and its degradation products in a pharmaceutical formulation. Int J Pharm Pharm Sci 2012;4(3):299-303.
Khaja P, Asgar A, Shanana B, Syed AH. Reverse phase-HPLC method for the analysis of tinidazole in the pharmaceutical dosage form and bulk drug. Int J Pharm Pharm Sci 2010;2:46-7.
Logoyda L. Validation of Chromatographic Methods of Analysis for the Determination of Active Pharmaceutical Ingredients in Different Medicines. Egypt: PharmaSchool Association for Pharmaceutical Development and Scientific Research; 2016. p. 34.
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