QUANTITATIVE DETERMINATION OF AMLODIPINE FROM CACO-2 CELL MONOLAYERS BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHYâ€“MASS SPECTROMETRY/MASS SPECTROMETRY

Liliya Logoyda

doi:10.22159/ajpcr.2018.v11i6.24971

Authors

Liliya Logoyda Department of Pharmaceutical Chemistry, I. Horbachevsky Ternopil State Medical University, Ternopil, Ukraine.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i6.24971

Keywords:

Amlodipine besylate, Nil, Caco-2 cells, Bioavailability

Abstract

Objective: A simple, rapid high-performance liquid chromatographyâ€“mass spectrometry/mass spectrometry (MS/MS) method was developed for the determination of amlodipine from confluent Caco-2 monolayers and from aqueous solution.

Methods: Chromatography was achieved on Discovery C18, 50Ã—2.1 mm, 5 Î¼m column. Samples were chromatographed in a gradient mode (eluent A [acetonitrile-water-formic acid, 5:95:0.1 v/v], eluent B [acetonitrile-formic acid, 100:0.1 v/v]). The initial content of the eluent B is 0%, which increases linearly by 1.0 min to 100% and to 1.01 min returns to the initial 0%. The mobile phase was delivered at a flow rate of 0.400 ml/min into the MS electrospray ionization chamber. The sample volume was 5 Î¼l.

Results: Under these conditions, amlodipine was eluted at 1.61 min. According to the Caco-2 test results, amlodipine appeared to have moderate to high permeability. Recovery value for amlodipine is 52.33%. Caco-2 permeability values for amlodipine are in agreement with BCS Class I classification for these drugs and their known high bioavailability in humans.

Conclusion: A new rapid method was developed for the determination of amlodipine from confluent Caco-2 monolayers and from aqueous solution. Acquired results demonstrate that proposed strategy can be effortlessly and advantageously applied for the examination of amlodipine from Caco-2 cell monolayers.

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