AN INSIGHT ON ALGAL CELL DISRUPTION FOR BIODIESEL PRODUCTION
DOI:
https://doi.org/10.22159/ajpcr.2018.v11i2.22481Keywords:
Biodiesel, Cell disruption, Microalgae, MacroalgaeAbstract
 Objective: This review article deals with the effect that various cell disruption techniques have on the efficiency of lipid extraction. We have reviewed existing algal cell disruption techniques that aid the biodiesel production process.
Methods: Current rise in demand for energy has led the researcher to focus on the production of sustainable fuels, among which biodiesel has received greater attention. This is due to its larger lipid content, higher growth rate, larger biomass production, and lower land use. Extraction of lipid from algae (micro and macro) for the production of biodiesel involves numerous downstream processing steps, of which cell wall disruption is a crucial step. Bead milling, high-pressure homogenization, ultra-sonication, freeze-drying, acid treatment, and enzymatic lysis are some methods of cell disruption. The cell disruption technique needs to be optimized based on the structure and biochemical composition of algae.
Result: The lipid extraction efficiency varies depending on the algal species and the cell disruption technique used.
Conclusion: In-depth research and development of new techniques are required to further enhance the cell disruption of the algal cell wall for the enhanced recovery of lipids. In addition, the operating costs and energy consumption should also be optimized for the cost-effective recovery.
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Patil V, Tran KQ, Giselrød HR. Towards sustainable production of biofuels from microalgae. Int J Mol Sci 2008;9:1188-95.
Bajhaiya A, Mandotra S, Suseela M, Toppo K, Ranade S. Algal biodiesel: The next generation biofuel for India. Asian J Exp Biol Sci 2010;1:728-39.
Khan O, Khan ME, Kumar A, Kumar S. Study on some advanced techniques to produce biodiesel from non-edible oils. Int J Adv Res Innov 2017;5:34-8.
Sunil K, Avneesh P, Kunal M. Screening and growth kinetics studies of wild chlorophycean fresh water microalgal species for biomass and biofuel application. Int J Pharm Pharm Sci 2014;7:312-21.
Sukahara KT, Awayama SS. Liquid fuel production using microalgae. J Jpn Pet Inst 2005;48:251-9.
Saldivar RP. Algae biofuels production processes, carbon dioxide fixation and biorefinery concept. J Pet Environ Biotechnol 2014;5:184.
Wang H, Liu G, Ruan R, Liu Y. Biofuel from microalgae: Current status, opportunity and challenge. Proc Int Conf Mater Environ Eng 2014;66:1388-1403.
Zemke-White WL,Clements KD, Harris PJ. Acid lysis of macroalgae by marine herbivorous fishes: Effects of acid pH on cell wall porosity. J Exp Mar Bio Ecol 2000;245:57-68.
Zheng H, Yin J, Gao Z, Huang H, Ji X, Dou C, et al. Disruption of chlorella vulgaris cells for the release of biodiesel-producing lipids: A comparison of grinding, ultrasonication, bead milling, enzymatic lysis, and microwaves. Appl Biochem Biotechnol 2011;164:1215-24.
Northcote DH, Goulding KJ, Horne RW. The chemical composition and structure of the cell wall of chlorella pyrenoidosa. Biochem J 1958;70:391-7.
Scott SA, Davey MP, Dennis JS, Horst I, Howe CJ, Lea-Smith DJ, et al. Biodiesel from algae: Challenges and prospects. Curr Opin Biotechnol 2010;21:277-86.
Demirbas A. Importance of biodiesel as transportation fuel. Energy Policy 2007;35:4661-70.
Campbell MN. Biodiesel : Algae as a renewable source for liquid fuel. Guelph Eng J 2008;1:2-7.
Gong Y, Jiang M. Biodiesel production with microalgae as feedstock: From strains to biodiesel. Biotechnol Lett 2011;33:1269-84.
Chisti Y. Biodiesel from microalgae beats bioethanol. Trends Biotechnol 2008;26:126-31.
Roessler PG, Brown LM, Dunahay TG, Heacox DA, Jarvis EE, Schneider JC, et al. Genetic engineering approaches for enhanced production of biodiesel fuel from microalgae. Enzym Convers Biomass Fuels Prod 1994;566:255-70.
Chisti Y, Moo-Young M. Disruption of microbial cells for intracellular products. Enzym Microb Technol 1986;8:194-204.
Kumar RR, Rao PH, Arumugam M. Lipid extraction methods from microalgae: A comprehensive review. Front Energy Res 2015;2:61.
Lee SJ, Yoon BD, Oh HM. Rapid method for the determination of lipid from the green alga Botryococcus braunii. Biotechnol Tech 1998;12:553-6.
Geciova J, Bury D, Jelen P. Methods for disruption of microbial cells for potential use in the dairy industry - A review. Int Dairy J 2002;12:541-53.
Doucha J, LÃvanský K. Influence of processing parameters on disintegration of chlorella cells in various types of homogenizers. Appl Microbiol Biotechnol 2008;81:431-40.
Greenwell HC, Laurens LM, Shields RJ, Lovitt RW, Flynn KJ. Placing microalgae on the biofuels priority list: A review of the technological challenges. J R Soc Interface 2010;7:703-26.
Postma PR, Suarez-Garcia E, Safi C, onathana KY, Olivieri G, Barbosa MJ, et al. Bioresource technology energy efficient bead milling of microalgae : Effect of bead size on disintegration and release of proteins and carbohydrates. Bioresour Technol 2017;224:670-9.
Hopkins TR. Physical and chemical cell disruption for the recovery of intracellular proteins. Bioprocess Technol 1991;12:57-83.
Miranda JR, Passarinho PC, Gouveia L. Bioethanol production from scenedesmus obliquus sugars: The influence of photobioreactors and culture conditions on biomass production. Appl Microbiol Biotechnol 2012;96:555-64.
Krisnangkura K. Simple method for estimation of cetane index of vegetable oil methyl esters. J Am Oil Chem Soc 1986;63:552-3.
Munir N, Sharif N, Shagufta N, Saleem F, Manzoor F. Harvesting and processing of microalgae biomass fractions for biodiesel production (a review). Sci Tech Dev 2013;32:235-43.
Kim J, Yoo G, Lee H, Lim J, Kim K, Kim CW, et al. Methods of downstream processing for the production of biodiesel from microalgae. Biotechnol Adv 2013;31:862-76.
Shen Y, Pei Z, Yuan W, Mao E. Effect of nitrogen and extraction method on algae lipid yield. Int J Agric Biol Eng 2009;2:51-7.
Petrier C, Jiang Y, Lamy MF. Ultrasound and environment: Sonochemical destruction of chloroaromatic derivatives. Environ Sci Technol 1998;32:1316-8.
Günerken E, D’Hondt E, Eppink MH, Garcia-Gonzalez L, Elst K, Wijffels RH, et al. Cell disruption for microalgae biorefineries. Biotechnol Adv 2015;33:243-60.
Lee AK, Lewis DM, Ashman PJ. Disruption of microalgal cells for the extraction of lipids for biofuels: Processes and specific energy requirements. Biomass Bioenergy 2012;46:101.
Spiden EM, Yap BH, Hill DR, Kentish SE, Scales PJ, Martin GJ, et al. Quantitative evaluation of the ease of rupture of industrially promising microalgae by high pressure homogenization. Bioresour Technol 2013;140:165-71.
Olmstead IL, Hill DR, Dias DA, Jayasinghe NS, Callahan DL, Kentish SE, et al. A quantitative analysis of microalgal lipids for optimization of biodiesel and omega-3 production. Biotechnol Bioeng 2013;110:2096-104.
Yap BH, Crawford SA, Dumsday GJ, Scales PJ, Martin GJ. A mechanistic study of algal cell disruption and its effect on lipid recovery by solvent extraction. Algal Res 2014;5:112-20.
Halim R, Gladman B, Danquah MK, Webley PA. Oil extraction from microalgae for biodiesel production. Bioresour Technol 2011;102:178-85.
Samarasinghe N, Fernando S, Lacey R, Faulkner WB. Algal cell rupture using high pressure homogenization as a prelude to oil extraction. Renew Energy 2012;48:300-8.
Balasubramanian RK, Doan TT, Obbard JP. Factors affecting cellular lipid extraction from marine microalgae. Chem Eng J 2013;215:929-36.
Wang G, Wang T. Characterization of lipid components in two microalgae for biofuel application. J Am Oil Chem Soc 2012;89:135-43.
Chemat F, Zill-e-Huma, Khan MK. Applications of ultrasound in food technology: Processing, preservation and extraction. Ultrason Sonochem 2011;18:813-35.
Suslick KS, Flannigan DJ. Inside a collapsing bubble: Sonoluminescence and the conditions during cavitation. Annu Rev Phys Chem 2008;59:659-83.
Pal A, Verma A, Kachhwaha SS, Maji S. Biodiesel production through hydrodynamic cavitation and performance testing. Renew Energy 2010;35:619-24.
Joannes C, Sipaut CS, Dayou J, Yasir SM, Mansa RF. The potential of using pulsed electric field (PEF) technology as the cell disruption method to extract lipid from microalgae for biodiesel production. Int J Renew Energy Res 2015;5:598-21.
Brujan EA, Nahen K, Schmidt P, Vogel A. Dynamics of laser-induced cavitation bubbles near an elastic boundary. Fluid Mech 2001;433:251-81.
Engler CR. Disruption of microbial cells. Princ Biotechnol Eng Consid 1985;2:305-24.
Halim R, Hosikian A, Lim S, Danquah MK. Chlorophyll extraction from microalgae: A review on the process engineering aspects. Int J Chem Eng 2010;2010:1-11.
Adam F, Abert-Vian M, Peltier G, Chemat F. Solvent-free†ultrasound-assisted extraction of lipids from fresh microalgae cells: A green, clean and scalable process. Bioresour Technol 2012;114:457-65.
Mason TJ, Lorimer JP, Bates DM, Zhao Y. Dosimetry in sonochemistry: The use of aqueous terephthalate ion as a fluorescence monitor. Ultrason Sonochem1994;1:S91-5.
Krehbiel JD, Schideman LC, King DA, Freund JB. Algal cell disruption using microbubbles to localize ultrasonic energy. Bioresour Technol 2014;173:448-51.
Sheng J, Vannela R, Rittmann BE. Disruption of synechocystis PCC 6803 for lipid extraction. Water Sci Technol 2012;65:567-73.
Pan X, Niu G, Liu H. Comparison of microwave-assisted extraction and conventional extraction techniques for the extraction of tanshinones from Salvia miltiorrhiza bunge. Biochem Eng J 2002;12:71-7.
Terigar BG, Balasubramanian S, Boldor D, Xu Z, Lima M, Sabliov CM, et al. Continuous microwave-assisted isoflavone extraction system: Design and performance evaluation. Bioresour Technol 2010;101:2466-71.
Balasubramanian S, Allen JD, Kanitkar A, Boldor D. Oil extraction from scenedesmus obliquus using a continuous microwave system - Design, optimization, and quality characterization. Bioresour Technol 2011;102:3396-403.
Wahidin S, Idris A, Shaleh SR. Rapid biodiesel production using wet microalgae via microwave irradiation. Energy Convers Manag 2014;84:227-33.
Prabakaran P, Ravindran AD. A comparative study on effective cell disruption methods for lipid extraction from microalgae. Lett Appl Microbiol 2011;53:150-4.
Qin S, Timoshkin IV, Michelle M, Wilson MP, MacGregor SJ, Given MJ, et al. Pulsed electric field treatment of microalgae: Inactivation tendencies and energy consumption. IEEE Trans Plasma Sci 2014;42:3191-6.
Alexandra D, Fernandes S. Assessment of a Pulsed Electric Field based Treatment for Marine Microalgae Cell Disruption. Diss. Master Dissertation in Biological Engineering, Instituto Superior Técnico, Universidade de Lisboa; 2015.
Eing C, Goettel M, Straessner R, Gusbeth C, Frey W. Pulsed electric field treatment of microalgae - Benefits for microalgae biomass processing. IEEE Trans Plasma Sci 2013;41:2901-7.
Klimek-Ochab M, Brzezińska-Rodak M, Zymańczyk-Duda E, Lejczak B, Kafarski P. Comparative study of fungal cell disruption – scope and limitations of the methods. Folia Microbiol (Praha) 2011;56:469-75.
Vogels G, Kula MR. Combination of enzymatic and/or thermal pretreatment with mechanical cell disintegration. Chem Eng Sci 1992;47:123-31.
Pourmortazavi SM, Hajimirsadeghi SS. Supercritical fluid extraction in plant essential and volatile oil analysis. J Chromatogr A 2007;1163:2-4.
Guldhe A, Singh B, Rawat I, Ramluckan K, Bux F. Efficacy of drying and cell disruption techniques on lipid recovery from microalgae for biodiesel production. Fuel 2014;128:46-52.
Prakash P, Raja SN. Evaluation of protein release rate frommycoprotien - Fusarium venenatum by cell disruption method. Int J Pharm Pharm Sci 2014;6:491-3.
Halim R, Rupasinghe TW, Tull DL, Webley PA. Mechanical cell disruption for lipid extraction from microalgal biomass. Bioresour Technol 2013;140:53-63.
Daroch M, Geng S, Wang G. Recent advances in liquid biofuel production from algal feedstocks. Appl Energy 2013;102:1371-81.
Halim R, Danquah MK, Webley PA. Extraction of oil from microalgae for biodiesel production: A review. Biotechnol Adv 2012;30:709-32.
Andrews BA, Asenjo JA. Enzymatic lysis and disruption of microbial cells. Trends Biotechnol 1987;5:273-7.
Vanthoor-Koopmans M, Wijffels RH, Barbosa MJ, Eppink MH. Biorefinery of microalgae for food and fuel. Bioresour Technol 2013;135:142-9.
Sander K, Murthy G. Enzymatic degradation of microalgal cell walls. ASABE Anu Int Meet 2009;300:12.
Wu C, Xiao Y, Lin W, Li J, Zhang S, Zhu J, et al. Aqueous enzymatic process for cell wall degradation and lipid extraction from nannochloropsis sp. Bioresour Technol 2017;223:312-6.
Wang D, Li Y, Hu X, Su W, Zhong M. Combined enzymatic and mechanical cell disruption and lipid extraction of green alga neochloris oleoabundans. Int J Mol Sci 2015;16:7707-22.
Koberg M, Cohen M, Ben-amotz A, Gedanken A. Bioresource technology bio-diesel production directly from the microalgae biomass of nannochloropsis by microwave and ultrasound radiation. Bioresour Technol 2011;102:4265-9.
Dhar DW, Saha S. Cell disruption methods for improving lipid extraction efficiency in unicellular microalgae. Eng Life Sci 2015;15:443-7.
Zbinden MD, Sturm BS, Nord RD, Carey WJ, Moore D, Shinogle H, et al. Pulsed electric field (PEF) as an intensification pretreatment for greener solvent lipid extraction from microalgae. Biotechnol Bioeng 2013;110:1605-15.
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