ANTITUBERCULOSIS ACTIVITY OF EXTRACT AND FRACTIONS OF TINOSPORA CRISPA AGAINST MYCOBACTERIUM TUBERCULOSIS H37RV USING MYCOBACTERIA GROWTH INDICATOR TUBE AND AGAR PROPORTION METHOD

Retno Wahyuningrum; Ritmaleni Ritmaleni; Tatang Irianti; Subagus Wahyuono; Takushi Kaneko

doi:10.22159/ajpcr.2018.v11i3.22587

Authors

Retno Wahyuningrum Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Indonesia.
Ritmaleni Ritmaleni Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Indonesia.
Tatang Irianti Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Indonesia.
Subagus Wahyuono Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Indonesia.
Takushi Kaneko Tuberculosis Alliance, New York, United States.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i3.22587

Keywords:

Mycobacterium tuberculosis, Tinospora crispa, Tuberculosis activity

Abstract

Â Objective: The increasing incidence of multidrug-resistant tuberculosis (TB) has created a need to discover a new anti-TB drug candidates. The aim of this study was to screen extract and fractions of Tinospora crispa for activity against Mycobacterium tuberculosis H37Rv.

Methods: The dried and pulverized T. crispa stem was extracted by maceration method using ethanol (96%). The anti-TB activity was carried out using mycobacteria growth indicator tube (MGIT) system and agar proportion method with Lowensteinâ€“Jensen (LJ) medium.

Result: The result of this study showed that ethanolic extract and fractions of T. Crispa did not exhibit anti-TB activity in the range of 100â€“1000 Î¼g/ml with MGIT method, while with agar proportion method, there were M. tuberculosis colonies growth on the LJ containing 1000 Î¼g/ml extract slants.

Conclusion: The tested extract and fractions of T. crispa have no anti-TB activity against M. tuberculosis until 1000 Î¼g/ml.

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