TYROSINASE INHIBITION, 2,2-DIPHENYL-1-PICRYLHYDRAZYL RADICAL SCAVENGING ACTIVITY, AND PHYTOCHEMICAL SCREENING OF FRACTIONS AND ETHANOL EXTRACT FROM LEAVES AND STEM BARK OF MATOA (POMETIA PINNATA)

Rani Sauriasari; Nur Azizah; Katrin Basah

doi:10.22159/ajpcr.2017.v10s5.23105

Authors

Rani Sauriasari Department of Pharmacy , Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia
Nur Azizah Department of Pharmacy , Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia.
Katrin Basah Department of Pharmacy , Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia.

DOI:

https://doi.org/10.22159/ajpcr.2017.v10s5.23105

Abstract

Objective: This study aims to investigate the potency of matoa as a tyrosinase inhibitor and antioxidant and also to identify the chemical compounds in the most active fraction and an ethanol extract from the leaves and stem bark of matoa.

Methods: The extracts were tested for their tyrosinase inhibitory activity by evaluating the formation of L-dopachrome at 490 nm. Antioxidant activity was tested using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The most active extract from liquid-liquid partition analysis was fractionated, and then, the assays were performed.

Results: The ethanol extract of leaves and stem bark of matoa showed weak anti-tyrosinase activity (percent inhibition was 24.54Â±0.22% and 21.93Â±0.57%, respectively, final concentration 200 Î¼g/mL), but they showed strong DPPH radical scavenging activity (IC50 values were 6.11 Î¼g/mL and 5.47 Î¼g/mL, respectively). The ethyl acetate fraction was the most active fraction with an IC50 value of 5.38 Î¼g/mL. Ethanol extract from the leaves and stem bark of matoa and the ethyl acetate fraction contains flavonoids, tannins, saponins, triterpenoids, and glycosides.

Conclusion: Matoa does not have potency as a tyrosinase inhibitor, but it has good antioxidant activity, although still lower than that of quercetin.

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