ASSESSMENT OF PHYSICOCHEMICAL PROPERTIES AND PHYTOCHEMICAL CONSTITUENTS ALONG WITH ANTIBACTERIAL POTENTIAL OF TARENNA POLYCARPA LEAF FRACTIONS
DOI:
https://doi.org/10.22159/ajpcr.2018.v11i4.23641Keywords:
Tarenna polycarpa, Fractions, Antimicrobial, Phytochemical, Physicochemical, Agar well diffusion methodAbstract
 Objective: The aim was to investigate the physicochemical analysis, preliminary phytochemical screening and further evaluation of its antibacterial activity from morbesi-besi (Tarenna polycarpa) leaf ethyl acetate and hexane fractions (HF).
Methods: The physicochemical parameters were carried out as per the World Health Organization (WHO) guideline from its ethanolic extract. The phytochemical screening was performed as per Indonesia Pharmacopoeia and conventional method on its ethyl acetate and HF. Ethyl acetate and HF at different concentration were evaluated using agar well diffusion assay.
Result: The plant was found to be free from contaminations. Morbesi-besi leaf fractions are composed of flavonoids, glycosides, saponins, tannins, steroids, and triterpenoids. The phytochemical screening showed only ethyl acetate of lotus leaf extract contains the flavonoids. The antibacterials assay showed that ethyl acetate fraction was better than HF from the inhibition zone.
Conclusion: From the outcomes of this study, morbesi-besi leaf (T. polycarpa) possess potential antibacterial activity.
Downloads
References
Haro G, Sinaga SM, Iksen I, Nerdy N, Theerachetmongkol S. Protective effects of chives leaves (Allium schoenoprasum, L.) infusion against ethylene glycol and ammonium chloride induced nephrolithiasis in rats. J Appl Pharm Sci 2017;7:222-5.
Hepsibah HA, Jothi JA. In vitro antibacterial activity and phytochemical analysis of Ormocaprum cochin Chinese extracts on nosocomial infection causing bacteria. Asian J Pharm Clin Res 2015;8:113-6.
Rahmaningsih S, Prajitno A, Aulanniam A, Maftuch M. Bioactive compounds from majapahit fruit (Crescentia cujete) as a potential natural antibacterial. Int J ChemTech Res 2017;10:90-9.
Barus BR. Antibacterial Activities Ethanol Extract and Fraction Leaf Morbesi-besi (Tarenna polycarpa (Miq.) Koord. ex Valenton) to Some Bacteria. Thesis Faculty of Pharmacy, University of Sumatera Utara; 2017. p. 1-105.
Su PW, Yang CH, Yang JF, Su PY, Chuang LY. Antibacterial activities and antibacterial mechanism of Polygonum cuspidatum extracts against nosocomial drug-resistant pathogens. Molecules 2015;20:11119-30.
Sinulingga SE. Angiogenesis Effect of Ethyl Acetate Fraction Ointment of Karamunting Leaves on Rat Wound that Infected by Bacteria. Thesis Faculty of Pharmacy, University of Sumatera Utara; 2017. p. 1-72.
Ahmed D, Saeed R, Shakeel N, Fatima K, Arshad A. Antimicrobial activities of methanolic extract of Carissa opaca roots and its fractions and compounds isolated from the most active ethyl acetate fraction. Asian Pasific J Trop Biomed 2015;5:541-45.
Depkes RI. Materia medika. Ditjen POM 1995;6:297-307.
Farnsworth NR. Biologycal and phytochemical screening of plants. J Pharm Sci 1996;55:225-76.
Harbone JB. Metode fitokimia. ITB 1987;2:6, 49.
World Health Organization (WHO). Quality Control Methods for Medicinal Plant Materials. WHO/PHARM/92.559. Geneva: World Health Organization (WHO); 1998. p. 4-46.
Milala MA, Warabe AM, Ali AA, Gulani IA. Antimicrobial activities of aqueous and ethanolic leaves extracts of Ficus platyphylla del. Arch Appl Sci Res 2015;7:37-42.
NCCLS. Performance Standard for Antimicrobial Susceptibility Testing. Weyne, PA. USA: NCCLS; 2001.
Oyebode JA, Fajilade TO. Antibacterial activities of aquesous and ethanolic extract of Allium cepa (Onion bulb) against some selected pathogenic microorganisms. Int J Sci Res 2014;4:1-3.
World Health Organization (WHO). Quality Control Methods for Medicinal Plant Materials. Geneva: WHO; 1992. p. 31-3.
Karthikkumaran S, Sajeesh T, Parimelazhagan T, Vinodhkumar V, Kamalanathan D, Natarajan T. Evaluation of antioxidant and antimicrobial activities of Tarenna asiatica. Asian J Pharm Clin Res 2014;7 Suppl 1:102-10.
Aguado MI, Dudik NG, Zamora CM, Torres CA, Nunez MB. Antioxidant and antibacterial activities of hydroalcoholic extracts from Aloysia polystachya griseb moldenke and Lippia turbinata Griseb (Verbenaceae). Int J Pharm Pharm Sci 2016;8:393-5.
Shetty VG, Patil MG, Dound AS. Evaluation of phytochemical and antibacterial properties of Calotropis procera (Ait) R. Br. leaves. Int J Pharm Pharm Sci 2015;7:316-9.
Published
How to Cite
Issue
Section
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.