IN VITRO BIOLOGICAL PROPERTIES OF CRUDE METHANOL EXTRACT FROM MUSHROOM; FLAMMULINA VELUTIPES (GOLDEN NEEDLE MUSHROOM)

Bajaree Jantrapanukorn; Pannapa Powthong; Chitradee Luprasong

doi:10.22159/ajpcr.2018.v11i9.25509

Authors

Bajaree Jantrapanukorn Faculty of Medical Technology, Rangsit University, Pathum Thani, 12000, Thailand.
Pannapa Powthong Faculty of Medical Technology, Rangsit University, Pathum Thani, 12000, Thailand.
Chitradee Luprasong Sun Herb, Thai, Chinese Manufacturing, Rangsit University, Pathum Thani, 12000, Thailand.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i9.25509

Keywords:

Flammulina velutipes, Methanol crude extract, Pharmacological properties, Antioxidant, Antimicrobial, Antitumor

Abstract

Objectives: The objectives of this study were to investigate the in vitro biological effect of the methanolic crude extract received from edible mushrooms; Flammulina velutipes on anti-inflammatory, antioxidant activity, anti-lipid peroxidation (LPO), antimicrobial activity, and anticancer activity. The scientific result obtained here was possibility of developing this variety as an ingredient in health/medical food purposes as well as a functional food.

Methods: In this study, the methanol crude extract of F. velutipes has been performed and in vitro pharmacological properties such as anti-inflammatory, antioxidant activity, anti-LPO, antimicrobial activity, and anticancer activity were evaluated.

Results: The results revealed that anti-inflammatory activity of crude extract was very significant with 79.81Â±0.87% of the dose-dependent manner. Antioxidant and anti-low-density lipoprotein (LDL) peroxidation activity of the extract showed its capacity of free radical scavenging with high-level antioxidant compounds and lipid preventive effects (2, 2-diphenyl-1-picrylhydrazyl: 79.81Â±0.01%, total phenol: 991.10Â±0.01 Î¼g gallic acid equivalents/0.1 g dry matter, H2O2: 93.27Â±0.05%, LPO: 33.33Â±0.00%, and LDL: 57Â±0.03%, respectively). Analysis has also demonstrated that the methanolic extract of F. velutipes provided an antimicrobial activity to ESBL-producing Klebsiella pneumoniae. Moreover, F. velutipes extract showed a growth inhibitory effect in HepG2 cells with the 50% inhibitory concentration (IC50) value IC50=8.25Â±7.59 Î¼g/ml.

Conclusion: This indicated that crude extract from F. velutipes was scientifically proved as a potential source of therapeutic agent and be able to utilize as a functional ingredient for healthy or supplement food.

Downloads

Download data is not yet available.

References

Mau JL, Lin HC, Chen CC. Antioxidant properties of several medicinal mushrooms. J Agric Food Chem 2002; 50:6072-77.

Dubois V, Breton S, Linder M, Fanni J, Parmentier M. Fatty acid profiles of 80 vegetables oils with regard to their nutritional potential. Eur J Lipid Sci Technol 2007;109:710-32.

Elmastas M, Isildak O, Turkekul I, Temur N. Determination of antioxidant activity and antioxidant compounds in wild edible mushrooms. J Food Comp Anal 2007;20:337-45.

Turkoglu A, Duru ME, Mercan N, Kivrak I, Gezer K. Antioxidant and antimicrobial activities of Laetiporus sulphureus (Bull) Murrill. Food Chem 2007;101:267-73.

Puttaraju NG, Venkateshaiah SU, Dharmesh SM, Urs SM, Somasundaram R. Antioxidant activity of indigenous edible mushrooms. J Agric Food Chem 2006;54:9764-72.

Leon F, Quintana J, Rivera A, Estevez F, Bermejo J. Lanostanoid triterpenes from Laetiporus sulphureus and apoptosis induction on HL- 60 human myeloid occurring cells. J Nat Prod 2004;67:2008-11.

Tseng YH, Yang JH, Mau JL. Antioxidant properties of polysaccharides from Ganoderma tsugae. Food Chem 2008;107:732-8.

Yang W, Fang Y, Liang J, Hu Q. Optimization of ultrasonic extraction of Flammulina velutipes polysaccharides and evaluation of its acetylcholinesterase inhibitory activity. Food Res Int 2011;44:1269-75.

Leung MY, Fung KP, Choy YM. The isolation and characterization of an immunomodulatory and antitumor polysaccharide preparation from Flammulina velutipes. Immunopharmacology 1997;35:255-63.

Mizushima Y, Kobayashi M. Interaction of anti-inflammatory drugs with serum proteins, especially with some biologically active proteins. J Pharm Pharm 1968;20:169-73.

Jung BK, Jong BK, Kang JC, Gabriele MK, Anthony DW. Antioxidant activity of 3, 4, 5-trihydroxybenzaldehyde isolated from Geum japonicum. J Food Drug Anal 2006;14:190-3.

Singleton VL, Orthofer R, Lamuela-Raventos RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol 1999;29:152-78.

Halliwell B, Gutteridge JM, Arugma OI. The deoxyribose method: A simple Test-Tubeâ€ assay for determination of rate constants for reactions of hydroxyl radicals. Analy Biochem 1987;165:215-9.

Ruberto G, Baratta MT, Deans SG, Dorman HJ. Antioxidant and antimicrobial activity of Foeniculum vulgare and Crithmum maritimum essential oils. Planta Med 2000;66:687-93.

Cardile V, Renis M, Scifo C, Lombardo L, Gulino R, Mancari B, et al. Behaviour of the new asbestos amphibole fluor-edenite in different lung cell systems. Int J Biochem Cell Biol 2004;36:849-60.

Perumal R, Dey A, Manavalan R, Prakasam K, Jayachandran E, Sreenivasa GM. Inhibition of albumin denaturation and anti inflammatory activity of furfuryl substituted pyrimidinoimidazolinones. Int J Chem Sci 2008;6:2016-22.

Rosner H, Williams LA, Jung A, Kraus W. Disassembly of microtubules and inhibition of neurite outgrowth, neuroblastoma cell proliferation, and MAP kinase tyrosine dephosphorylation by dibenzyl trisulphide. Biochim Biophys Acta 2001;1540:166-77.

Williams LA, Roesner H, Conrad J, Moeller W, Beifuss U, Chiba K, et al. Selected secondary metabolites from the phytolaccaceae and their biological/ pharmaceutical significance. Recent Res Dev Phytochem 2002;6:13-68.

Kawabata T, Packer L. Alpha-lipoate can protect against glycation of serum albumin, but not low density lipoprotein. Biochem Biophys Res Commun 1994;203:99-104.

Williams LA, Vasquez EA, Milan PP, Zebitz C, Kraus W. In vitro anti-inflammatory and antimicrobial activities of phenylpropanoids from Piper betle L. (Piperaceae). In: Rauter AP, Palma FB, Justino J, AraÃºjo ME, dos Santos SP, editors. Natural Products in the New Millennium: Prospects and Industrial Application. Proceedings of the Phytochemical Society of Europe. Vol. 47. Dordrecht: Springer; 2002. p. 221-7.

SÅ‚awiÅ„ska A, Radzki W, Kalbarczyk J. Antioxidant activities and polyphenolics content of Flammulina velutipes mushroom extracts. Herb Pol 2013;59:26-35.

MilovanovicÂ´ I, StanojkovicÂ´ T, StajicÂ´ M, VukojevicÂ´J, KneÅ¾evicÂ´ A. The effect on biological activity of Flammulina velutipes. Ital J Food Sci 2015;27:1-7.

Zhang Z, Lv G, Pan H, Fan L, Soccol CR, Pandey A. Production of powerful antioxidant supplements via solid-state fermentation of wheat (Triticum aestivum Linn.) by Cordyceps militaris. Food Technol Biotechnol 2012;50:32-9.

Badmus JA, Adedosu TO, Fatoki JO, Adegbite VA, Adaramoye OA, Odunola OA. Lipid peroxidation inhibition and antiradical activities of some leaf fractions of Mangifera indica. Acta Pol Pharm 2011;68:23-9.

Siddhuraju P, Becker K. The antioxidant and free radical scavenging activities of processed cowpea (Vigna unguiculata (L.) Walp. seed extracts. Food Chem 2007;101:10-9.

Tripathy SS, Rajoriya A, Mahapatra A, Gupta N. Biochemical and antioxidant properties of wild edible mushrooms used for food by tribal of Eastern India. Int J Pharm Pharm Sci 2016;8:194-9.

Halliwell B, Gutteridge JM. Free Radicals in Biology and Medicine. 3rd ed. Oxford: Oxford University Press; 1999.

Chance B, Sies H, Boveris A. Hydroperoxide metabolism in mammalian organs. Physiol Rev 1979;59:527-605.

Steinberg D, Parthasarathy S, Carew TE, Khoo JC, Witztum JL. Beyond cholesterol. Modifications of low-density lipoprotein that increase its atherogenicity. N Engl J Med 1989;320:915-24.

Cross CE, Halliwell B, Borish ET, Pryor WA, Ames BN, Saul RL, et al. Oxygen radicals and human disease. Ann Intern Med 1987;107:526-45.

Rahman MA, Abdullah N, Aminudin N. Antioxidative effects and inhibition of human low density lipoprotein oxidation in vitro of polyphenolic compounds in Flammulina velutipes (golden needle mushroom). Oxid Med Cell Longev 2015;2015:1-10.

Nicolcioiu MB, Popa G, Matei F. Antimicrobial activity of ethanolic extracts made of mushroom mycelia developed in submerged culture. Sci Bull F Biotechnol 2017;21:159-64.

Senthilkumar V, Sathishkumar G, Sivaramakrishnan S, Sujatha K, Razia M. Evaluation of phytoconstituents, in vitro antioxidant and antimicrobial activities of edible white button mushroom Agaricus bisporus. Int J Pharm Pharm Sci 2016;8:67-71.

SÃ¡nchez-Camargo AP, Herrero M. Rosemary (Rosmarinus officinalis) as a functional ingredient: Recent scientific evidence. Curr Opin Food Sci 2017;14:13-9.

Natarajan N, Thamaraiselvan R, Lingaiah H, Srinivasan P, Periyasamy BM. Effect of flavonone hesperidin on the apoptosis of human mammary carcinoma cell line MCF-7. Biomed Prev Nutr 2011;1:207-15.