Screening of active antimicrobial and biological enzymes of microbial isolated from soil in Thailand

Pannapa Powthong; Apichai Sripean; Pattra Suntornthiticharoen

doi:10.22159/ajpcr.2017.v10i4.15454

Authors

Pannapa Powthong
Apichai Sripean
Pattra Suntornthiticharoen

DOI:

https://doi.org/10.22159/ajpcr.2017.v10i4.15454

Abstract

Objective: The objectives of this study were to isolate microorganisms and screen for potential antimicrobial activities from the soil.Â
Methods: In this study, a total of 425 isolates were isolated from 100 soil samples. The preliminary screening for antimicrobial activities of theseÂ isolates was performed by modified cross-streak, agar diffusion, and modified icrodilution technique against 16 pathogenic bacteria and fungi.
Results: In the anti-microbial activity, there were three isolates, namely, 277, 303, and 307 exhibited inhibitory activity against methicillin-resistant
Staphylococcus aureus and Salmonella typhimurium respectively. This study also examined the various enzymes producing from soil microorganismsÂ including chitinase, chitosanase, amylase, cellulose, caseinase, gelatinase, esterase, and lipase production of different selective media for 24 and 48 hrs using the direct spot method. The results revealed that 28 isolates could produce various enzymes with strong activity. Most of them producedÂ gelatinase (5.65%) and caseinase (5.18%). There were four isolates that produce broad-spectrum enzyme. In addition, the investigation of selected microorganism identification showed that they can be divided into three groups: Burkholderia spp., Pseudomonas spp., and Rhodococcus spp.
Conclusion: This study demonstrated that the microorganisms from soil are capable of producing potential, antibacterial, and bioactive enzymes.

Keywords: Antimicrobial activity, Extracellular enzyme, Soil microbial, Drug-resistant bacteria.

Downloads

Download data is not yet available.

References

Riley MA, Wertz JE. Bacteriocins: Evolution, ecology, and application. Annu Rev Microbiol 2002;56:117-37.

El-Banna N, Quddoumi SS, Daradka H. Antimicrobial substances produced by bacteria isolated from different Jordanian sources that are active against methicillin-resistant Staphylococcus aureus. Afr J Biotechnol 2007;6(15):1837-9.

Desriac F, JÃ©gou C, Balnois E, Brillet B, Le Chevalier P, Fleury Y. Antimicrobial peptides from marine proteobacteria. Mar Drugs 2013;11(10):3632-60.

Mohapatra BR, Bapuji M, Sree A. Production of industrial enzymes (amylase, carboxymethylcellulase and protease) by bacteria isolated from marine sedentary organisms. Acta Biotechnol 2003;23:75-84.

SÃ¡nchez-Porro C, MartÃn S, Mellado E, Ventosa A. Diversity of moderately halophilic bacteria producing extracellular hydrolytic enzymes. J Appl Microbiol 2003;94(2):295-300.6. Dang H, Zhu H, Wang J, Li T. Extracellular hydrolytic enzyme screening of culturable heterotrophic bacteria from deep-sea sediments of the Southern Okinawa Trough. World J Microbiol Biotechnol 2009;25(1):71-9.

Vaz AB, Rosa LH, Vieira ML, de Garcia V, BrandÃ£o LR, Teixeira LC, et al. The diversity, extra-cellular enzymatic activities and photoprotective compounds of yeasts isolated in Antarctica. Braz J Microbiol 2011;42(3):937-47.

Emimol A, Ganga G, Parvathy R, Radhika G, Nair GM. Screening of microbes producing extracellular hydrolytic enzyme from corporation waste dumping site and house hold waste for the enhancement of bioremediation methods. IOSR J Pharm Biol Sci 2012;4(1):54-60.

MacNeil IA, Tiong CL, Minor C, August PR, Grossman TH, Loiacono KA, et al. Expression and isolation of antimicrobial small molecules from soil DNA libraries. J Mol Microbiol Biotechnol 2001;3(2z):301-8.

Torsvik V, Ã˜vreÃ¥s L. Microbial diversity and function in soil: From genes to ecosystems. Curr Opin Microbiol 2002;5(3):240-5.

Fernando C. Screening tests for antibiotics. Mycologia 1947;39:128-30.

National Committee for Clinical Laboratory Standards (NCCLS). In: 12th Informational Supplement M-100-S12. Wayne, PA: NCCLS; 2002.

National Committee for Clinical Laboratory Standards. In: Approved Standard. NCCLS Document M38-A. Wayne, PA: NCCLS; 2002.

National Committee for Clinical Laboratory Standards. In: Approved Standard M27-A2. 2nd ed. Wayne, PA: NCCLS; 2002.

Hankin L, Anagnostakis SL. The use of solid media for the detection of enzyme production by fungi. Mycologia 1975;67(3):597-607.

Smibert RM, Krieg NR. Phenotypic Characterization in Methods for General and Molecular Bacteriology. Washington, DC: American Society for Microbiology; 1994. p. 611-51.

Hendricks CW, Doyle JD, Hugley B. A new solid medium for enumerating cellulose-utilizing bacteria in soil. Appl Environ Microbiol 1995;61(5):2016-9.

Saadoun I, Gharaibeh R. The Streptomyces flora of Badia region of Jordan and its potential as a source of antibiotic resistant bacteria. J Arid Environ 2003;53(3):365-71.

Motta AS, Cladera-Olivera F, Brandelli A. Screening for antimicrobial activity among bacteria isolated from the Amazon Basin. Braz J Microbiol 2004;35(4):307-10.

Zuhud EA, Rahayu WP, Wijaya CH, Sari PP. Antimikrobialaktivity of kedawungekstrak (Parkia roxburghii G. Don) on food borne pathogens. J Teknol Ind Pangan 2001;12:6-12.

Ajizah A, Dan Mirhanuddin T. Potential of Eusideroxylon zwageri T. et B. Bark extract to inhibit the growth of Staphylococcus aureus in in vitro. Bioscientiae 2007;4:37-42.

Hospital-acquired pneumonia in adults: Diagnosis, assessment of severity, initial antimicrobial therapy and preventative strategies: A consensus statement. Am J Respir Crit Care Med 1996;153(5):1711-25.

Mashoria A, Lovewanshi HS, Rajawat BS. Isolation of antimicrobial producing bacteria from soil samples collected from Bhopal Region of Madhya Pradesh, India. Int J Curr Microbiol Appl Sci 2014;3(12):563-9.

Kaur S, Kaur J, Pankaj PP. Isolation and characterization of antibiotic producing microorganisms from soil samples of certain area of Punjab Region of India. Int J Pharm Clin Res 2014;6(4):312-5.

Duo-Chuan L. Review of fungal chitinases. Mycopathologia 2006;161(6):345-60.

Alves PD, Siqueira Fde F, Facchin S, Horta CC, VictÃ³ria JM, Kalapothakis E. Survey of microbial enzymes in soil, water, and plant microenvironments. Open Microbiol J 2014;8(6):25-31.

Lee SW, Won K, Lim HK, Kim JC, Choi GJ, Cho KY. Screening for novel lipolytic enzymes from uncultured soil microorganisms. Appl Microbiol Biotechnol 2004;65(6):720-6.

Barman D, Saud ZA, Habib MR, Islam MF, Hossain K, Yeasmin T. Isolation of cellulytic bacterial strains from soil for effective and efficient bioconversion of solid waste. Life Sci Med Res 2011;25:1-6.

Fujii K, Oosugi A, Sekiuchi S. Cellulolytic microbes in the Yanbaru, a subtropical rainforest with an endemic biota on Okinawa Island, Japan. Biosci Biotechnol Biochem 2012;76(5):906-11.