IDENTIFICATION OF BIOACTIVE COMPONENTS IN ENHALUS ACOROIDES SEAGRASS EXTRACT BY GAS CHROMATOGRAPHYâ€“MASS SPECTROMETRY

Amudha P; Jayalakshmi M; Pushpabharathi N; Vanitha V

doi:10.22159/ajpcr.2018.v11i10.25577

Authors

Amudha P Department of Biochemistry, School of Life Sciences, Vels University, Pallavaram, Chennai, Tamil Nadu, India.
Jayalakshmi M Department of Biochemistry, School of Life Sciences, Vels University, Pallavaram, Chennai, Tamil Nadu, India.
Pushpabharathi N Department of Biochemistry, School of Life Sciences, Vels University, Pallavaram, Chennai, Tamil Nadu, India.
Vanitha V Department of Biochemistry, School of Life Sciences, Vels University, Pallavaram, Chennai, Tamil Nadu, India.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i10.25577

Keywords:

Enhalus acoroides, Gas chromatography-mass spectroscopy, Phytocompounds, Biological activity, 1-Nonadecene, n-tetracosanol-1

Abstract

Objective: This study deals with the determination of possible phytocompounds present in the ethyl acetate extract of Enhalus acoroides using gas chromatography-mass spectroscopy (GC-MS) technique.

Methods: Using GC-MS technique the phytocompounds present in the ethyl acetate extract of E. acoroides whole seagrass was investigated, and the mass spectra of the compounds found in the extract were matched with the National Institute of Standards and Technology library.

Results: GC-MS analysis of E. acoroides extract revealed the existence of several phytocompounds which includes 1-nonadecene (17.15%), n-tetracosanol-1 (11.48%), 1-octadecene (10.06%), 2-pentadecanone (7.87%), behenyl alcohol (7.33%), 17-pentatriacontene (4.84%), triacontane (4.25%), tetratetracontane (4.17%), and butylated hydroxytoluene (2.09%).

Conclusion: E. acoroides possess distinct phytocompounds such as 1-nonadecene and n-tetracosanol-1 which possess antioxidant property, triacontane which has antibacterial, antidiabetic and antitumor activities. Further studies need to elute novel bioactive compounds and toxicity profile through animal models.

Â

Downloads

Download data is not yet available.

Author Biography

Amudha P, Department of Biochemistry, School of Life Sciences, Vels University, Pallavaram, Chennai, Tamil Nadu, India.

Department of Biochemistry, Head and Associate Professor

References

Tapsell LC, Hemphill I, Cobial L. Health benefit of herbs and species. The past, the present, the future. Med J Austria 2006;188(4):4-24.

Amudha P, Bharathi NP, Vanitha V. Caesalpinia bonducellaâ€“a review on pharmacological and phytochemical activity of seeds. Int J Pharm Bio Sci 2016;7:674-80.

Tabacco E, Borreani G, Crovetto GM, Galassi G, Colombo D, Cavallarin L, et al. Effect of chestnut tannin on fermentation quality, proteolysis, and protein rumen degradability of alfalfa silage. J Dairy Sci 2006;89:4736-46.

Amudha P, Varadharaj V. Phytochemical and pharmacological potential of Annona species: A review. Asian J Pharm Clin Res 2017;10:1-8.

Christopher B. RHSA-Z Encylopedia of Garden Plants. 3rd ed. London: Dorling Kindersley; 2003. p. 738-51.

Hites AR. Gas Chromatography Mass Spectroscopy: Handbook of Instrumental Techniques for Analytical Chemistry. New Jersey: Prentice Hall PTR; 1997. p. 609-11.

Jayalakshmi M, Amudha P, Vanitha V. Assessment of minerals from shrimp shell waste. Res Pharm Sci 2017;8:194-7.

Arsad A, Bujang JS, Zakaria MH. Distribution and Significance of Seagrass Ecosystems in Malaysia. Malaysia: Aquatic Ecosystem Health and Management Society; 2006. p. 203-14.

Hartog CD, Kuo J. Taxonomy and biogeography of seagrasses. In: Larkum AW, Orth RJ, Duarte CM, editors. Seagrass. Springer, Berlin: Biology, Ecology and Conservation; 2006. p. 691.

Amudha P, Vanitha V. Phytochemical analysis and in vitro antioxidant screening of sea grass-Enhalus acoroides. Int J Res Pharm Sci 2017;8:1 8.

Gillan FT, Hogg RW, Drew EA. The sterol and fatty acid compositions of seven tropical seagrasses from North Queensland, Australia. Phytochemistry 1984;23:2817-21.

Hemalatha S, Amudha P, Bharathi NP, Vanitha V. Determination of bioactive phytocomponents from hydroethanolic extract of Annona squamosa (Linn.) Leaf by Gc-Ms. Int J Pharm Sci Res 2017;8:2539-44.

Mathi P, Nikhil K, Das S, Roy P, Bokka VR, Botlagunta M. Evaluation of in vitro anticancer activity and GC-MS analysis from leaf Sophora Interrupta Bedd. Int J Pharm Pharm Sci 2015;7:303-8.

Saravanan P, Chandramohan G, Mariajancyrani J, Shanmugasundaram P. Gc-Ms analysis of phytochemical constituents in ethanolic bark extract of Ficus religiosa Linn. Int J Pharm Pharm Sci 2014;6:457-60.

Harbourne JB. Phytochemical Methods. A Guide to Modern Techniques of Plant Analysis. 2nd ed. London: Chapman and Hall; 1984. p. 4-120.

Wagner H, Bladt S, Zgainski EM. Plant Drug Analysis. Berlin: Springer-Verlag; 1984. p. 298-334.

Amaral AC, Gomes LA, Sila JR, Ferreira JL, Ade SR, Mdo SR, et al. Liposomal formulation of turmerone-rich hexane fractions from Curcuma longa enhances their antileishmanial activity. Biomed Res Int 2014;doi: 10.1155/2014/694934.

Rukachaisirikul T, Siriwattanakit P, Sukcharoenphol K, Wongvein C, Ruttanaweang P, Wongwattanavuch P, et al. Chemical constituents and bioactivity of Piper sarmentosum. J Ethnopharmacol 2004;93:173-6.

Lee YS, Kang MH, Cho SY, Jeong CS. Effects of constituents of Amomum xanthioides on gastritis in rats and on growth of gastric cancer cells. Arch Pharm Res 2007;30:436-43.

Mishra PM. Sree A. Antibacterial activity and GC-MS analysis of the extract of leaves of Finlaysonia obovata (A mangrove plant). Asian J Plant Sci 2007;6:168-72.

Kumar PP, Kumaravel S, Lalitha C. Screening of antioxidant activity, total phenolics and GC-MS study of Vitex negundo. Afr J Biochem 2010;4:191-5.

Yehye WA, Rahman NA, Ariffin A, Hamid SB, Alhadi AA, Kadir FA, et al. Understanding the chemistry behind the antioxidant activities of butylated hydroxytoluene (BHT): A review. Eur J Med Chem 2015;101:295-312.

Committee for Orphan Medicinal Products. Public Summary of Opinion of Orphgan designation. Phenol, 4- (2-(amino methyl)-4- thiazoyl)-2, 6-bis (1,1- dimethylethyl) Monohydrochloride for the Treatment of Huntingtonâ€™s Disease; 2015.

Arancibia LA, Naspi CV, Pucci GN, Arce ME.,Colloca CB. Biological activity of 1-heneicosanol isolated from Senecio coluhuapiensis, an endemic species from Patagonia. Pharm Chem J 2016;3:73-7.

Abubacker MN, Devi PK. In vitro antifungal potentials of bioactive compounds heptadecane, 9- hexyl and Ethyl iso-allocholate isolated from Lepidagathis cristata Wild. (Acanthaceae) leaf. BBB 2015;3:336 43.

Lakshmi M, Nair BR. GC-MS analysis of the chloroform extract of bark of Terminalia travancorensi Wight and Arn. (Combretaceae). Int J Pharm Sci Res 2017;8:794-8.

Ertas A, Yilmaz MA, Firat M. Chemical profile by LC-MS/MS, GC/MS and antioxidant activities of the essential oils and crude extracts of two euphorbia species. Nat Prod Res 2015;29:529-34.

Mallick SS, Dighe VV. Detection and estimation of alpha-amyrin, beta-sitosterol, lupeol and n-tricontane in two medicinal plants by high performance thin layer chromatography. Adv Chem 2014; doi. org/10.1155/2014/143948.

Mammen D, Daniel M, Sane RT. Seasonal and geographical variations in chemical constituents of Leptadenia reticulate. Int J Pharm Sci Rev Res 2010;4:111-6.

Rao MR, Phillips S, Kumar MH, Saranya Y, Divya D, Prabhu K. GC-MS analysis, antimicrobial, antioxidant activity of an ayurvedic medicine, Salmali niryasa. J Chem Pharm Res 2015;7:131-9.

Jegajeevanram P, Alhaji NM, Kumarave S. Identification of pesticide compounds of Cynodon dactylon by GC-MS analysis. Int J Pharm Bio Sci 2014;5:604-8.

Kandutsch AA, Chen HW, Heiniger HJ. Biological activity of some oxygenated sterols. Science 1978;201:498-501.

Alam MS, Chopra N, Ali M. Niwa M. Oleanen and stigmasterol derivatives from Ambroma augusta. Phytochemistry 1996;41:1197 200.

Chandramohan A, Divya RS. Comparison of antioxidant activity in Gracilaria edulis and Hypnea valentiae. IJARIIT 2017;3:294-6.

Prakash A, Suneetha V. Punica granatum (Pomegranate) rind extract as a potent substitute for L- ascorbic acid with respect to the antioxidant activity. Res J Pharm Biol Chem Sci 2014;5:597.

Nelson JA, Steckbeck SR, Spencer TA. Biosynthesis of 24,25-epoxycholesterol from squalene 2,3;22,23-dioxide. J Biol Chem 1981;256:1067-8.

Panini SR, Sexto RC, Rudney H. Regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase by oxysterol by-products of cholesterol biosynthesis. Possible mediators of low density lipoprotein action. J Biol Chem 1984;259:7767-71.