THE EFFECTIVENESS OF COLEUS AMBOINICUS LEAF EXTRACTS AS AN ANALGETIC ACTIVITY ON MICE EXPOSURED TO ACETIC ACID

Yunita Sari Pane; Sufitni Sufitni; Fitriani Lumongga; Nurfida Alrasyid; Dini Permata Sari; Ridha Wati; Mohammad Basyuni

doi:10.22159/ajpcr.2018.v11i9.26538

Authors

Yunita Sari Pane Department of Pharmacology & Therapeutic, Universitas Sumatera Utara, Medan, North Sumatera, Indonesia. http://orcid.org/0000-0003-1120-8571
Sufitni Sufitni Department of Anatomy, Universitas Sumatera Utara, Medan, North Sumatera, Indonesia.
Fitriani Lumongga Department of Anatomy, Universitas Sumatera Utara, Medan, North Sumatera, Indonesia.
Nurfida Alrasyid Department of Parasitology, Universitas Sumatera Utara, Medan, North Sumatera, Indonesia.
Dini Permata Sari Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan 20155, Indonesia.
Ridha Wati Department of Forestry, Faculty of Forestry, Universitas Sumatera Utara, Jl. Tri Dharma Ujung No. 1 Medan, North Sumatra, 20155, Indonesia.
Mohammad Basyuni Department of Forestry, Faculty of Forestry, Universitas Sumatera Utara, Jl. Tri Dharma Ujung No. 1 Medan, North Sumatra, 20155, Indonesia.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i9.26538

Keywords:

Bangun-bangun leaves (Coleus amboinicus), Analgetic, Ethanol extract, Leukocytes, Writhing response, Leukocyte migration

Abstract

Objective: This study aims to observe the analgesic activity of Bangun-bangun (Coleus amboinicus) leaves by induction of pain arising from chemical excitement after exposure to acetic acid.

Methods: The effectiveness test of water extract and ethanol extract of Bangun-bangun leaves as an analgesic was measured by comparing the mean of quantitative and the writhing between control and treatment group. In addition, observations on the decrease in inflammatory processes occurred by calculating the reduction in the number of leukocyte migration to the inflammatory area.

Result: The decrease of excitatory pain in all treatment Groups (I-IV) were significantly different. The comparison of mean values Â± SEM decreased excitatory pain Groups I-II (255.00Â±22.22; 88.33Â±14.58), p=0.000. Groups I-III (255.00Â±22.22; 60.00Â±10.04), p=0.000 and Groups I-IV (255.00Â±22.22; 50.83Â±3.09), p=0.000. On histopathology examination, all treatment groups were significantly different (p=0.006). The comparison of mean value Â± SEM decrease number of leukocyte Groups I-II (31.73Â±5.22; 14.70Â±3.71), p=0.025. Groups I-III (31.73Â±5.22; 15.67Â±3.22), p=0.036 and Groups I-IV (31.73Â±5.22; 11.77Â±2.77), p=0.008. However, the comparison of decrease of excitatory of pain and number of leukocyte migration between each group did not show significantly different (p>0.05).

Conclusions: This study concluded that the Bangun-bangun leaves both in the form of water extract and ethanol extract proved efficacious as a pain reliever in the mice after induction of acetic acid.

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References

Jacob L, Kostev K. Prevalence of pain medication prescriptions in France, Germany, and the UK-a cross-sectional study including 4,270,142 patients. J Postgrad Med 2018;130:32-6.

Sutrisno E, Adnyana K, Sukandar EY, Fidrianny I, Aligita W. Anti-inflammatory study of Anredera cordifolia leaves and Centella asiatica herbs and its combination using human red blood cell-membrane stabilization method. Asian J Pharm Clin Res 2016;9:78-80.

Mills S, Bone K. Principle and Practice of Phytotherapy Modern Herbal Medicine. Seattle: Churchill of Washington; 2000.

Santosa CM, Hertiani T. Chemical compound and wilted leaves extract effect (Coleus amboinicus L.) on neutrophil rodent phagocytic activity of white rat (Rattus norvrgicus). Pharm Mag Indones 2005;16:141-8.

Gunawan A. Comparison of analgesic effect between paracetamol with combination of paracetamol and caffeine in mice. J Biomed 2009;1:37 43.

Katzung BG, Trevor AJ. Basic and Clinical Pharmacology. 12th ed. San Francisco: McGraw Hill Education Lange; 2015.

Silitonga M. Lactagogum Effect of Cumin Leaves (Coleus amboinicus, L.) in Lactation Mice. Masterâ€™s Thesis of Science, Biology Study Program. Bogor: Bogor Agricultural University; 1993.

Tatiya AU, Saluja AK, Kalaskar MG, Surana SJ, Patil PH. Evaluation of analgesic and anti-inflammatory activity of Bridelia retusa (Spreng) bark. J Tradit Complement Med 2017;7:441-51.

Widhianata AH. Efek Analgesik Jus Umbi Wortel (Daucus Carota L) Pada Mencit Putih Betina, Skripsi. Yogyakarta: Program Studi Ilmu Farmasi Fakultas Farmasi Universitas Sanata Dharma; 2007.

GuimarÃ£es AG, Xavier MA, Santana MT, Camargo EA, Santos CA, Brito FA, et al. Carvacrol attenuates mechanical hypernociception and inflammatory response. Naunyn Schimiedebergâ€™s Arch Pharmacol 2012;385:253-63.

Basyuni M, Kinjo Y, Baba S, Shinzato N, Iwasaki H, Siregar EB, et al. Isolation of salt tolerance genes from roots of mangrove plant, Rhizophora stylosa Griff., using PCR-based suppression subtractive hybridization. Plant Mol Biol Rep 2011;29:533-43.

Lima MD. Anti-inflammatory effects of carvacrol: Evidence for a key role of interleukin-10. Eur J Pharmacol 2013;699:112-7.

Fachini QF, Kummer R, Estevao SC. Effects of thymol and carvacrol, constituents of Thymus vulgaris L. essential oil, on the inflammatory response. Evid Based Complement Altern Med 2012;2012:1-10.

Robles JA, Yu GF. The anti-asthmatic effect of the combined yerba buena (Mentha arvensis Linn.) and oregano (Coleus amboinicus Lour.) leaves in BALB/c mice model of allergic asthma. Acta Med Philipp 2017;51:86.

Laaboudi W, Ghanam J, Aissam H, Merzouki M, Benlemlih M. Anti-inflammatory and analgesic activities of olive tree extract. Int J Pharm Pharm Sci 2016;8:414-9.

Shaikh S, Joshi YM, Kadam V. Comparative study of anti-inflammatory activity if aqueous and methanolic extracts of Hibiscus cannabinus leaf (malvaceae). Int J Pharm Pharm Sci 2016;8:64-8.

Lincy MP, Mohan VR, Jeeva S. Anti-inflammatory activity of aerial part of Maerua apetala roth (Jacobs) against carrageenan induced paw edema. Int J Curr Pharm Res 2014;5:131-7.