THE EFFECTIVENESS OF SOURSOP LEAF EXTRACT AGAINST GROWTH OF AGGREGATIBACTER ACTINOMYCETEMCOMITANS ATCCÂ® 6514â„¢ IN VITRO

Ameta Primasari; Minasari Nasution; Nurul Hidayati Arbi; Dini Permata Sari; Mohammad Basyuni

doi:10.22159/ajpcr.2018.v11i12.28435

Authors

Ameta Primasari Departement of Oral Biology, Fakultas Kedokteran Gigi, Universitas Sumatera Utara, Jl. Alumni No. 2 Kampus USU Padang Bulan Medan 20155, Indonesia. http://orcid.org/0000-0003-1120-8571
Minasari Nasution Departement of Oral Biology, Fakultas Kedokteran Gigi, Universitas Sumatera Utara, Jl. Alumni No. 2 Kampus USU Padang Bulan Medan 20155, Indonesia.
Nurul Hidayati Arbi Departement of Oral Biology, Fakultas Kedokteran Gigi, Universitas Sumatera Utara, Jl. Alumni No. 2 Kampus USU Padang Bulan Medan 20155, Indonesia.
Dini Permata Sari Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan 20155, Indonesia.
Mohammad Basyuni Department of Forestry, Faculty of Forestry, Universitas Sumatera Utara, Jl. Tri Dharma Ujung No. 1 Medan, North Sumatra, 20155, Indonesia.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i12.28435

Keywords:

Soursop leaf extract, Antibacterial, Nil, Minimum inhibitory concentration, Minimum bactericidal concentration

Abstract

Objective: The objective of this study was to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) antibacterial power of soursop leaf extract on Aggregatibacter actinomycetemcomitans (Aa) ATCCÂ® 6514â„¢ growth.

Methods: This study was experimental laboratory with post-test only control group design and consists of 8 treatment groups that were soursop leaf extract group with concentration 50%, 25%, 12.5%, 6.25%, 3.125%, and 1.5625% as well as negative control groups were brain heart infusion broth (BHIB) media and chlorhexidine as positive controls. Each treatment was done 3 repetitions. Testing the effectiveness of soursop leaf extract using dilution methods on BHIB and subculture media on Mueller Hinton Agar (MHA) media. The number of Aa ATCCÂ® 6514 â„¢ colonies was calculated manually using the total plate count method on the MHA media. Data were analyzed using Kruskalâ€“Wallis test (p<0.05) followed by least significance different (LSD) test to see the significant mean difference between treatment groups.

Results: Concentration of MIC from soursop leaf extract on Aa ATCCÂ® 6514â„¢ growth was 1.5625% and MBC was 6.25%. LSD assay results showed significant difference effect (p<0.05) Aa ATCCÂ® 6514â„¢ from each treatment group.

Conclusion: Soursop leaf extract has antibacterial effectivity against Aa ATCCÂ® 6514 â„¢.

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References

Adewole S, Ojewole J. Protective effects of Annona muricata Linn. (Annonaceae) leaf aqueous extract on serum lipid profiles and oxidative stress in hepatocytes of streptozotocin-treated diabetic rats. Afr J Tradit Complement Altern Med 2009;6:30-41.

Coria-Tellez AV, Montalvo-GÃ³nzalez E, Yahia EM, Obledo- VÃ¡zquez EN. Annona muricata: A comprehensive review on its traditional medicinal uses, phytochemicals, pharmacological activities, mechanisms of action and toxicity. Arab J Chem 2016;11:662-91.

Gavamukulya Y, Abou-Elella F, Wamunyokoli F, AEl-Shemy H. Phytochemical screening, anti-oxidant activity and in vitro anticancer potential of ethanolic and water leaves extracts of Annona muricata (Graviola). Asian Pac J Trop Med 2014;7:S355-63.

Adefegha SA, Oyeleye SI, Oboh G. Distribution of phenolic contents, antidiabetic potentials, antihypertensive properties, and antioxidative effects of soursop (Annona muricata L.) fruit parts in vitro. Biochem Res Int 2015;2015:347673.

Sari DP, Basyuni M, Hasibuan PA, Wati R. The inhibition of polyisoprenoids from Nypa fruticans leaves on cyclooxygenase 2 expression of widr colon cancer cells. Asian J Pharm Clin Res 2018;11:154-7.

Basyuni M, Ginting PY, Lesmana I. Phytochemical analysis of binahong (Anredera cordifolia) leaves extract to inhibit in vitro growth of Aeromonas hydrophila. AIP Conf Proceed 2017;1904:020072.

Rahman FA, Haniastuti T, Utami TW. Skrining fitokimia dan aktivitas antibakteri ekstrak etanol daun sirsak (Annona muricata L.) pada Streptococcus mutans ATCC 35668. Maj Kedokt Gigi Indones 2017;3:1-7.

Vijayameena C, Subhashini G, Loganayagi M, Ramesh B. Phytochemical screening and assessment of antibacterial activity for the bioactive compounds in Annona muricata. Int J Curr Microbiol Appl Sci 2013;2:1-8.

Wahyuningtyas ED. Daya Hambat Ekstrak Daun Sirsak (Annona muricata L.) Terhadap Pertumbuhan Bakteri Plak Supragingiva. Surabaya, Indonesia: Universitas Airlangga; 2013.

Henderson B, Ward JM, Ready D. Aggregatibacter (Actinobacillus) actinomycetemcomitans: A triple a periodontopathogen? Periodontol 2000;54:78-105.

Pai BH, Rajesh G, Shenoy R, Rao A. Anti-microbial efficacy of soursop leaf extract (Annona muricata) on oral pathogens: An in-vitro study. J Clin Diagn Res 2016;10:ZC01-4.

Mardiana L, Buku TK. Daun Ajaib Tumpas Penyakit. Cibubur: Penebar Swadaya Grup; 2012.

Badrie N, Schauss AG. Soursop (Annona muricata L.): Composition, nutritional value, medicinal uses, and toxicology. In: Bioactive Foods in Promoting Health. New York: Elsevier; 2010. p. 621-43.

Schreiner HC, Sinatra K, Kaplan JB, Furgang D, Kachlany SC, Planet PJ, et al. Tight-adherence genes of are required for virulence in a rat model. Proc Natl Acad Sci 2003;100:7295-300.

Shetty SG, Hidayathulla S, Chandrashekar KR. Antimicrobial activity and phytochemical screening of Pterospermum reticulatum wight and arn. Int J Pharm Pharm Sci 2011;3:35-7.

Arunodaya HS, Krishna V, Shashikumar R, Girish Kumar K. Antibacterial and antioxidant activities of stem bark essential oil constituents of Litsea glutinosa C. B. ROB. Int J Pharm Pharm Sci 2016;8:258-64.

Tenggara FS, Rizka Y, Parisihni K. Daya hambat ekstrak daun sirsak (Annona muricata Linn) terhadap pertumbuhan bakteri mixed periodontopatogen. Susunan Redaksi 2014;8:103.

Malik R, Changela R, Krishan P, Gugnani S, Bali D. Virulence factors of Aggregatibacter actinomycetemcomitans-A status update. J Int Clin Dent Res Organ 2015;7:137-45.

Marsh P. Oral Microbiology. Vol. 1. Berlin: Springer; 2013.

Atikah AR, Budi HS, Kusumaningsih T. Antibacterial effects of 70% ethanol and water extract of cacao beans (Theobroma cacao L.) on Aggregatibacter actinomycetemcomitans. Dent J (Majalah Kedokt Gigi) 2016;49:104-9.

Pujiyanto S, Resdiani M, Raharja B, Ferniah RS. Î±-Amylase inhibitor activity of endophytic bacteria isolated from Annona muricata L. J Phys Conf Ser 2018;1025:12085.

Cushnie TP, Lamb AJ. Antimicrobial activity of flavonoids. Int J Antimicrob Agents 2005;26:343-56.

Jannah R, Husni MA, Nursanty R. Inhibition test of methanol extract from soursop leaf (Annona muricata Linn.) against Streptococcus mutans bacteria. J Nat 2017;17:23-30.

Dewi ZY, Nur A, Hertriani T. Efek antibakteri dan penghambatan biofilm ekstrak sereh (Cymbopogon nardus L.) terhadap bakteri Streptococcus mutans. Maj Kedokt Gigi Indones 2015;1:136-41.

Sumardi S, Basyuni M, Wati R. Antimicrobial activity of polyisoprenoids of sixteen mangrove species from North Sumatra, Indonesia. Biodiversitas 2018;19:1243-8.