EVALUATION OF ANTICANCER ACTIVITY OF CUCUMIS CALLOSUS AGAINST EHRLICH'S ASCITES CARCINOMA BEARING MICE

Siva Prasad Panda; Rajsekhar Reddy A; Uttam Prasad Panigrahy

doi:10.22159/ajpcr.2018.v11i10.27439

Authors

Siva Prasad Panda Department of Pharmacology, KL College of Pharmacy, KLEF Deemed to be University, Vaddeswaram, Guntur, Andhra Pradesh, India.
Rajsekhar Reddy A Department of Pharmacology, KL College of Pharmacy, KLEF Deemed to be University, Vaddeswaram, Guntur, Andhra Pradesh, India.
Uttam Prasad Panigrahy Department of Pharmacology, KL College of Pharmacy, KLEF Deemed to be University, Vaddeswaram, Guntur, Andhra Pradesh, India.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i10.27439

Keywords:

Cucumis callosus, Anticancer, Ehrlich Ascites Carcinoma cell lines

Abstract

Objective: Our previous research isolated Cucurbitacin B (CuB) and ebenone leucopentaacetate (ELP) from methanolic fruit extract of Cucumis callosus (MFCC). The fruits of C. callosus (Rottl.) Cogn. (Family: Cucurbitaceae) plant have been traditionally used for antioxidant, anti-inflammatory, and antidiabetic actions. The objective of this research was to evaluate in vitro and in vivo anticancer effect of MFCC on Ehrlich Ascites Carcinoma (EAC) cell lines.

Methods: In vitro anticancer assay of MFCC and standard drug, 5-fluorouracil (5-FU) was evaluated using Trypan blue and 3-(4, 5-dimethylthiazol-yl)-2, 5-diphenyl tetrazolium bromide methods. In vivo anticancer activity of MFCC and 5-FU was also performed after 24h of EAC cells (2Ã—106cells/ mouse) inoculation based on toxicity study for 9 consecutive days. The activity of the extract was assessed by the study of tumor volume, tumor weight, viable and non-viable cell count, hematological parameters, and biochemical estimations.

Results: The MFCC showed the direct antitumor effect on EAC cells in a dose-dependent manner with an IG50 value of 0.61 mg/ml. Furthermore, MFCC (350 mg/kg) exhibited significant (p<0.01) decrease in tumor volume, tumor weight, and viable cell count of EAC-treated mice. Hematological profile, biochemical estimation assay significantly (p<0.01) reverted to normal level in MFCC, and 5-FU treated mice.

Conclusion: The anticancer activity of fruits of C callosus is may be either due to the presence of CuB or/and ELP as phytoconstituent and the activity is comparable to standard drug 5-FU.

Downloads

Download data is not yet available.

Author Biography

Siva Prasad Panda, Department of Pharmacology, KL College of Pharmacy, KLEF Deemed to be University, Vaddeswaram, Guntur, Andhra Pradesh, India.

Associate professor, KL college of pharmacy, KLEF deemed to be University, Guntur, AP, INDIA

References

Abdullaev FI, Luna RR, Roitenburd BV, Espinosa AJ. The pattern of childhood cancer mortality in Mexico. Arch Med Res 2000;31:526-31.

Hussain MA, Pati S, Swain S, Prusty M, Kadam S, Nayak S, et al. Pattern and trends of cancer in Odisha, India: A retrospective study. Asian Pac J Cancer Prev 2012;13:6333-6.

Abdullaev FI. In: Plant-derived Agents Against Cancer, Gupta SK, editors. Pharmacology and Therapeutics in the New Millennium. New Delhi: Narosa Publishing House; 2001. p. 345-54.

Patwardhan B, Vaidya AD, Chorghade M. Ayurveda and natural products drug discovery. Curr Sci 2004;86:6.

Diwanay S, Gautam M, Patwardhan B. Cytoprotection and immunomodulation in cancer therapy. Curr Med Chem Anticancer Agents 2004;4:479-90.

Tripathy KD. Anticancer drugs. Essential Medical Pharmacology. 6th ed. New Delhi: Jaypee Brotherâ€™s Medical Publishers (P) Ltd.; 2007. p. 820.

Ramos AA, Prata-Sena M, Castro-Carvalho B, Dethoup T, Buttachon S, Kijjoa A, et al. Potential of four marine-derived fungi extracts as ant proliferative and cell death-inducing agents in seven human cancer cell lines. Asian Pac J of Trop Med 2015;8:798-806.

Bhattaram VA, Graefe U, Kohler C, Veit M, Derendorf H. Pharmacokinetic and bioavailability of herbal medicinal products. Phytomedicine 2002;9:1-33.

Hung HC, Joshipura KJ, Jiang R, Hu FB, Hunter D, Smith-Warner SA, et al. Fruit and vegetable intake and risk of major chronic disease. J Natl Cancer Inst 2004;96:1577-84.

Rathore M. Nutrient content of important fruit trees from arid zone of Rajasthan. J Hortic For 2009;1:103-8.

Raut M. Glimpses of Nature Series (No. 4) our Monsoon Plants, Bombay Natural History Society. Bombay, India; 1959.

Trivedi PC. Medicinal plants: Traditional knowledge. In: Sharma UK, Gogoi J, editors. Ethno-Medico-Botany of some Sacred Plants of Dhemaji District of Assam. New Delhi: IK International Pvt. Ltd.; 2006. p. 102.

Haldar PK, Kar B, Bala A, Bhattacharya S, Mazumder UK. Antitumor activity of Sansevieria roxburghiana rhizome against Ehrlich ascites carcinoma in mice. Pharm Biol 2010;48:1337-43.

Islam K, Ali SM, Jesmin M, Khanam JA. In vivo teratogenic effect of the mixture using the Zebrafish embryo developmental assay. Cancer Biol Med 2012;9:242-7.

Panda SP, Sarangi AK, Panigrahy UP. Isolation of cucurbitacin-B from Cucumis callosus and its hypoglycemic effect in isolated rat enterocytes. Int J Pharm Pharm Sci 2018;10:123-9.

Kausar H, Munagala R, Bansal SS, Aqil F, Vadhanam MV, Gupta RC, et al. Cucurbitacin B potently suppresses non-small-cell lung cancer growth: Identification of intracellular thiols as critical targets. Cancer Lett 2013;332:35-45.

Guo J, Wu G, Bao J, Hao W, Lu J, Chen X, et al. Cucurbitacin B induced ATM-mediated DNA damage causes G2/M cell cycle arrest in a ROS-dependent manner. PLoS One 2014;9:e88140.

Panda SP, Haldar PK, Bera S, Adhikary S, Kandar CC. Antidiabetic and antioxidant activity of Swietenia mahagoni in streptozotocin-induced diabetic rats. Pharm Biol 2010;48:974-9.

Bala A, Kara B, Haldar P, Mazumder U, Bera S. Evaluation of anticancer activity of Cleome gynandra on Ehrlichâ€™s ascites carcinoma treated mice. J Ethnopharmacol 2010;129:131-4.

Mossman T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods 1983;65:55-63.

Panda SP, Chakraborty M, Majumder P, Mazumder S, Das S, Haldar PK. The antidiabetic, antioxidant and anti-hyperlipidemic activity of Cucumis callosus in streptozotocin-induced diabetic rats. Int J Pharm Sci Res 2016;7:1978-84.

Prasad SB, Giri A. Antitumor effect of cisplatin against murine ascites daltonâ€™s lymphoma. Indian J Exp Biol 1994;32:155-62.

Arias JL. Novel strategies to improve the anticancer action of 5-fluorouracil by using drug delivery systems. Molecules 2008;13:2340 69.

Miura K, Kinouchi M, Ishida K, Fujibuchi W, Naitoh T, Ogawa H, et al 5-FU metabolism in cancer and orally-administrable 5-FU drugs. Cancers (Basel) 2010;2:1717-30.

Rajeshwar Y, Gupta M, Mazumder UK. Antitumor activity and in vivo antioxidant status of Mucuna pruriens (Fabaceae) seeds against Ehrlich ascites carcinoma in Swiss albino mice. Iranian J Pharmacol Ther 2005;4:46.

Chen JC, Chiu MH, Nie RL, Cordell GA, Qiu SX. Cucurbitacins and cucurbitane glycosides: Structures and biological activities. Nat Prod Rep 2005;22:386-99.

Militao GC, Dantas IN, Ferreira PM, Alves AP, Chaves DC, Monte FJ, et al. In vitro and in vivo anticancer properties of cucurbitacin isolated from Cayaponia racemose. Pharma Biol 2012;50:1479-87.

Duangmano S, Sae-Lim P, Suksamrarn A, Domann FE, Patmasiriwat P. Cucurbitacin B inhibits human breast cancer cell proliferation through disruption of microtubule polymerization and nucleophosmin/B23 translocation. BMC Complement Altern Med 2012;12:185.

Thoennissen NH, Iwanski GB, Doan NB, Okamoto R, Lin P, Abbassi S, et al. Cucurbitacin B induces apoptosis by inhibition of the JAK/STAT pathway and potentiates cytotoxic effects of gemcitabine on pancreatic cancer cells. Cancer Res 2009;69:5876-84.

Brantley EC, Benveniste EN. STAT-3: A molecular hub for signaling pathways in gliomas. Mol Cancer Res 2008;6:5.

Mukherjee C, Paul S, Kundu R. Comparative evaluation of ant proliferative activity of Solanum nigrum methanolic and aqueous extract on HELA, SIHA and C33A cells. Int J Pharm Pharm Sci 2015;7:320-4.

Kumar Y, Periyasamy L. GC-MS analysis and in-vitro cytotoxic studies of Bixa orellana seed extract against cancer cell line. Int J Pharm Pharm Sci 2016;8:408-13.