IN VITRO HYPOGLYCEMIC AND ANTIMICROBIAL ACTIVITY OF CUCUMIS CALLOSUS (ROTTL.) COGN. FRUIT
DOI:
https://doi.org/10.22159/ajpcr.2016.v9s2.11687Abstract
ABSTRACT
Objective: This research investigated in vitro hypoglycemic and antimicrobial activity of methanolic fruit extract of Cucumis callosus (MECC) (Rottl.)
cogn.
Methods: The in vitro antidiabetic ability of MECC was evaluated by inhibition studies of the digestive enzymes including α-amylase and α-glycosidase.
The antimicrobial activity of extract was further evaluated against 21 laboratory strains belongs to 8 bacterial species and 4 fungal species using cupplate
agar
diffusion assay
and agar
dilution assay.
Results: Our assay result suggested that the MECC exhibited dose-dependent increase in percentage inhibitory activity on α-glycosidase
(IC
50
405.37 µg/ml) and α-amylase enzymes (IC
721.55 µg/ml). The results of antimicrobial activity also showed that the extract inhibited the growth
of 13 strains of 7 bacterial species and all fungal species.
50
Conclusion: The observed inhibitions of α-amylase and α-glycosidase and inhibition of some microbial species suggested that the MECC may be useful
in the management of diabetes mellitus and treatment of some infectious diseases.
Keywords: Cucumis callosus, Diabetes, α-amylase, α-glycosidase, Antimicrobial.
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References
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