HARNESSING THE ANTIOXIDANT PROPERTY OF CERIUM AND YTTRIUM OXIDE NANOPARTICLES TO ENHANCE MESENCHYMAL STEM CELL PROLIFERATION

Hadeer A Aglan; Mostafa Mabrouk; Riham M Aly; Hanan H Beherei; Hanaa H Ahmed

doi:10.22159/ajpcr.2018.v11i9.27914

Authors

Hadeer A Aglan Stem Cells Lab, Center of Excellence for Advanced Sciences, National Research Centre, Giza, Egypt.
Mostafa Mabrouk Refractories, Ceramics and Building Materials Department, National Research Centre, Giza, Egypt.
Riham M Aly Stem Cells Lab, Center of Excellence for Advanced Sciences, National Research Centre, Giza, Egypt.
Hanan H Beherei Refractories, Ceramics and Building Materials Department, National Research Centre, Giza, Egypt.
Hanaa H Ahmed Stem Cells Lab, Center of Excellence for Advanced Sciences, National Research Centre, Giza, Egypt.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i9.27914

Keywords:

Mesenchymal stem cells, Cerium oxide nanoparticles, Yttrium oxide nanoparticles, Antioxidant effect, Proliferative impact

Abstract

Objective: This work was designed to explore if cerium oxide (CeO2) and yttrium oxide (Y2O3) nanoparticles as antioxidant agents could potentiate the proliferation of mesenchymal stem cells (MSCs) derived from human dental pulp (hDPSCs).

Methods: Nanoparticles were characterized by transmission electron microscopy, particle size and zeta potential, X-ray diffraction, Fourier-transform infrared spectroscopy, and scanning electron microscope (SEM) along with energy-dispersive X-ray spectrometry. Furthermore, MSCs were isolated from human dental pulp, propagated and characterized by flow cytometry. Thereafter, the proliferative impact of the suggested nanoparticles on hDPSCs was investigated by 3-(4,5)-dimethylthiazol)-2,5-diphenyl tetrazolium bromide assay.

Results: Different sizes (14.09â€“26.50 nm and 18.80â€“31.31 nm) for CeO2 and Y2O3 respectively, morphology, charges, and proliferative efficacy in hDPSCs were recorded for both nanoparticles.

Conclusion: Generally speaking, the tested nanoparticles heightened the proliferative response of hDPSCs with the most prominent effect exerted by 15 Î¼g/ml of CeO2 and 5 Î¼g/ml of Y2O3. It is reasonable to assume that the antioxidant property of CeO2 and Y2O3 be involved in strengthening the proliferation process of hDPSCs.

Downloads

Download data is not yet available.

References

Murray PE, Garcia-Godoy F, Hargreaves KM. Regenerative endodontics: A review of current status and a call for action. J Endod 2007;33:377-90.

Chen FM, Gao LN, Tian BM, Zhang XY, Zhang YJ, Dong GY, et al. Treatment of periodontal intrabony defects using autologous periodontal ligament stem cells: A randomized clinical trial. Stem Cell Res Ther 2016;7:33.

Zhang F, Ren T, Wu J, Niu J. Small concentrations of TGF-? 1 promote proliferation of bone marrow-derived mesenchymal stem cells via activation of Wnt/?-Catenin pathway. Indian J Exp Biol 2015;53:508 13.

Copland IB, Galipeau J. Death and inflammation following somatic cell transplantation. Semin Immunopathol 2011;33:535-50.

Trounson A, McDonald C. Stem cell therapies in clinical trials: Progress and challenges. Cell Stem Cell 2015;17:11-22.

Rouwkema J, Koopman B, Blitterswijk C, Dhert W, Malda J. Supply of nutrients to cells in engineered tissues. Biotechnol Genet Eng Rev 2010;26:163-78.

Muller F, Lustgarten M, Jang Y, Richardson A, Van Remmen H. Trends in oxidative aging theories. Free Radic Biol Med 2007;43:477-503.

Guin R, Banu S, Kurian GA. Synthesis of copper oxide nanoparticles using Desmodium gangeticum aqueous root extract. Int J Pharm Pharm Sci 2015;7:60-5.

Arya A, Gangwar A, Singh SK, Roy M, Das M, Sethy NK, et al. Cerium oxide nanoparticles promote neurogenesis and abrogate hypoxia-induced memory impairment through AMPK-PKC-CBP signaling cascade. Int J Nanomed 2016;23:1159-73.

Heckert EG, Karakoti AS, Seal S, Self WT. The role of cerium redox state in the SOD mimetic activity of nanoceria. Biomaterials 2008;29:2705-9.

Pirmohamed T, Dowding JM, Singh S, Wasserman B, Heckert E, Karakoti AS, et al. Nanoceria exhibit redox state-dependent catalase mimetic activity. Chem Commun (Camb) 2010;46:2736-8.

Das S, Singh S, Dowding JM, Oommen S, Kumar A, Sayle TX, et al. The induction of angiogenesis by cerium oxide nanoparticles through the modulation of oxygen in intracellular environments. Biomaterials 2012;33:7746-55.

Chang ML, Tie SL. Fabrication of novel luminor Y2O3: Eu3+@ SiO2 @ YVO4: Eu3+ with core/shell hetero nanostructure. Nanotechnology 2008;19:75711.

Hosseini A, Baeeri M, Rahimifard M, Navaei-Nigjeh M,Mohammadirad A, Pourkhalili N, et al. Antiapoptotic effects of cerium oxide and yttrium oxide nanoparticles in isolated rat pancreatic islets. Hum Exp Toxicol 2013;32:544-53.

Mitra RN, Merwin MJ, Han Z, Conley SM, Al-Ubaidi MR, Naash MI. Yttrium oxide nanoparticles prevent photoreceptor death in a light-damage model of retinal degeneration. Free Radic Biol Med 2014;75:140-8.

Gronthos S, Brahim J, Li W, Fisher LW, Cherman N, Boyde A, et al. Stem cell properties of human dental pulp stem cells. J Dent Res 2002;81:531-5.

van Meerloo J, Kaspers GJ, Cloos J. Cell sensitivity assays: The MTT assay. Methods Mol Biol 2011;731:237-45.

Li J, Zhang Z, Gao W, Zhang S, Ma Y, Qu Y. Pressure regulations on the surface properties of CeO2 nanorods and their catalytic activity for CO-oxidation and nitrile hydrolysis reactions. ACS Appl Mater Interfaces 2016;8:22988-96.

Soga K, Okumura Y, Tsuji K, Venkatachalam N. Effect of K3PO4 addition as sintering inhibitor during calcination of Y2O3 nanoparticle. J Phys 2009;191:1-7.

Mahapatra C, Singh RK, Lee JH, Jung J, Hyun JK, Kim HW. Nano-shape varied cerium oxide nanomaterials rescue human dental stem cells from oxidative insult through intracellular or extracellular actions. Acta Biomater 2017;50:142-53.

Farahmandjou M, Zarinkamar M, Firoozabadi TP. Synthesis of cerium oxide (CeO2) nanoparticles using simple CO-precipitation method. Rev Mex Fisic 2016;62:496-9.

Atta AH, El-Shenawy AI, Koura FA, Refat MS. Synthesis and characterization of some selenium nanometric compounds: Spectroscopic, biological and antioxidant assessments. World J Nano Sci Eng 2014;4:58-69.

Phoka S, Laokul P, Swatsitang E, Promarak V, Seraphin S, Maensiri S. Synthesis, structural and optical properties of CeO2 nanoparticles synthesized by a simple polyvinyl pyrrolidone (PVP) solution route. Mater Chem Phys 2009;115:423-8.

Jeong KJ, Bae DS. Synthesis and characterization of Y2O3 powders by a modified solvothermal process. Korean J Mater Res 2012;22:78-81.

Schwartz CA, Schwartz J. Surface modification of Y2O3 nanoparticles. Langmuir 2007;23:9158-916.

Chen YK, Huang AH, Chan AW, Shieh TY, Lin LM. Human dental pulp stem cells derived from different cryopreservation methods of human dental pulp tissues of diseased teeth. J Oral Pathol Med 2011;40:793 800.

Lutolf MP, Gilbert PM, Blau HM. Designing materials to direct stem-cell fate. Nature 2009;462:433-41.

Powar PV. Development status in the meadow of nanostructure magnetic drug delivery system and its promising applications. Int J Pharm Pharm Sci 2017;9:10-7.

Davison MJ, McMurray RJ, Smith CA, Dalby MJ, Meek RD. Nanopit-induced osteoprogenitor cell differentiation: The effect of nanopit depth. J Tissue Eng 2016;7:2041731416652778.

Valle-Prieto A, Conget PA. Human mesenchymal stem cells efficiently manage oxidative stress. Stem Cells Dev 2010;19:1885-93.

Li TS, MarbÂ´an E. Physiological levels of reactive oxygen species are required to maintain genomic stability in stem cells. Stem Cells 2010;28:1178-85.

Higuchi M, Dusting GJ, Peshavariya H, Jiang F, Hsiao ST, Chan EC, et al. Differentiation of human adipose-derived stem cells into fat involves reactive oxygen species and forkhead box o1 mediated upregulation of antioxidant enzymes. Stem Cells Dev 2013;22:878-88.

Perales-Clemente E, Folmes CD, Terzic A. Metabolic regulation of redox status in stem cells. Antioxid Redox Signal 2014;21:1648-59.

Mathieu J, Zhou W, Xing Y, Sperber H, Ferreccio A, Agoston Z, et al. Hypoxia-inducible factors have distinct and stage specific roles during reprogramming of human cells to pluripotency. Cell Stem Cell 2014;14:592-605.

Zhang Q, Ge K, Ren H, Zhang C, Zhang J. Effects of cerium oxide nanoparticles on the proliferation, osteogenic differentiation and adipogenic differentiation of primary mouse bone marrow stromal cells in vitro. J Nanosci Nanotechnol 2015;15:6444-51.

Popov AL, Tatarnikova OG, Popova NR, Selezneva II, Akkizov AY, Ermakov AM, et al. Ce1-Ñ…GdÑ…Oy nanoparticles stimulate proliferation of dental pulp stem cells in vitro. Nano Hybrids Composites 2017;13:26 31.

Colon J, Hsieh N, Ferguson A, Kupelian P, Seal S, Jenkins DW, et al. Cerium oxide nanoparticles protect gastrointestinal epithelium from radiation-induced damage by reduction of reactive oxygen species and upregulation of superoxide dismutase 2. Nanomedicine 2010;6:698 705.

Tsai YY, Oca-Cossio J, Agering K, Simpson NE, Atkinson MA, Wasserfall CH, et al. Novel synthesis of cerium oxide nanoparticles for free radical scavenging. Nanomedicine (Lond) 2007;2:325.

Colon J, Herrera L, Smith J, Patil S, Komanski C, Kupelian P, et al. Protection from radiation-induced pneumonitis using cerium oxide nanoparticles. Nanomedicine 2009;5:225.

Li Y, Li H, Zhang J, Zhao W, Shen J, Jiang D. In vitro evaluation of an yttria-stabilized zirconia reinforced nano-hydroxyapatite/polyamide 66 ternary biomaterial: Biomechanics, biocompatibility and bioactivity. RSC Adv 2016;6:114086-95.

Ghaznavi H, Najafi R, Mehrzadi S, Hosseini A, Tekyemaroof N, Shakeri-zadeh A, et al. Neuro-protective effects of cerium and yttrium oxide nanoparticles on high glucose-induced oxidative stress and apoptosis in undifferentiated PC12 cells. Neurol Res 2015;37:624-32.

Mandoli C, Pagliari F, Pagliari S, Forte G, Di Nardo P, Licoccia S, et al. Stem cell aligned growth induced by CeO2 nanoparticles in PLGA scaffolds with improved bioactivity for regenerative medicine. Adv Funct Mater 2010;20:1617.

Kanda Y, Hinata T, Kang SW, Watanabe Y. Reactive oxygen species mediate adipocyte differentiation in mesenchymal stem cells. Life Sci 2011;89:250-8.

Song H, Cha MJ, Song BW, Kim IK, Chang W, Lim S, et al. Reactive oxygen species inhibit adhesion of mesenchymal stem cells implanted into ischemic myocardium via interference of focal adhesion complex. Stem Cells 2010;28:555-63.

Jeong AY, Lee MY, Lee SH, Park JH, Han HJ. PPARdelta agonist-mediated ROS stimulates mouse embryonic stem cell proliferation through cooperation of p38 MAPK and wnt/beta-catenin. Cell Cycle 2009;8:611-9.

Lee SH, Lee YJ, Han HJ. Effect of arachidonic acid on hypoxia-induced IL-6 production in mouse ES cells: Involvement of MAPKs, NF-kappaB, and HIF-1alpha. J Cell Physiol 2010;222:574-85.

Kim J, Song S, Park S, Song S, Xia Y, Sung J. Primary involvement of NADPH oxidase 4 in hypoxia induced generation of reactive oxygen species in adipose derived stem cells. Stem Cells Dev 2012;21:2212 21.

Popov AL, Popova NR, Selezneva II, Akkizov AY, Ivanov VK. Cerium oxide nanoparticles stimulate proliferation of primary mouse embryonic fibroblasts in vitro. Mat Sci Eng C 2016;68:406-13.

Sun LY, Pang CY, Li DK, Liao CH, Huang WC, Wu CC, et al. Antioxidants cause rapid expansion of human adipose-derived mesenchymal stem cells via CDK and CDK inhibitor regulation. J Biomed Sci 2013;20:53.

Horie M, Nishio K, Kato H, Fujita K, Endoh S, Nakamura A, et al. Cellular responses induced by cerium oxide nanoparticles: Induction of intracellular calcium level and oxidative stress on culture cells. J Biochem 2011;150:461-71.

Gratton SE, Ropp PA, Pohlhaus PD, Luft JC, Madden VJ, Napier ME, et al. The effect of particle design on cellular internalization pathways. Proc Natl Acad Sci U S A 2008;105:11613-8.

Agarwal R, Roy K. Intracellular delivery of polymeric nanocarriers: A matter of size, shape, charge, elasticity and surface composition. Ther Deliv 2013;4:705-23.

Agarwal R, Singh V, Jurney P, Shi L, Sreenivasan SV, Roy K. Mammalian cells preferentially internalize hydrogel nanodiscs over nanorods and use shape specific uptake mechanisms. Proc Natl Acad Sci U S A 2013;110:17247-52.