EXTRACTION, ISOLATION AND CHARACTERIZATION OF BIOACTIVE FLAVONOIDS FROM THE FRUITS OF PHYSALIS PERUVIANA LINN EXTRACT
Abstract
Objective: Although Physalis peruviana Linn. fruit commonly known as golden berries, Cape gooseberries and wild tomato is highly esteemed
by consumers all over the world, scientific reports about the fruits are scarce and there was no systemic study in the literature regarding the
phytoconsituents present in the fruits of Indian P. peruviana L. Hence, the present study was aimed to determine the total flavonoids as well as
phenolic content in the fruits of P. peruviana L.
Methods: The ethanolic extract of P. peruviana L. fruits was subjected to phytochemical screening and the total flavonoids as well as phenolic contents
were estimated according to standard protocols. The extract was subjected to high-performance liquid chromatography analysis for the identification
of major flavonoids and the individual flavonoids were characterized by various spectral studies.
Results: The qualitative analysis revealed the presence of biologically active secondary metabolites such as phenols, flavonoids, glycosides, sterols,
saponins, tannins, lactones and alkaloids in the fruit extract. The fruit extract was also found to contain significant amounts of both phenolic
compounds, as well as flavonoids such as rutin, myricetin, quercetin and kaempferol.
Conclusion: The results of the present study indicate that many compounds rather than a single component of P. peruviana L. fruit is responsible for
its pharmacological as well as beneficial effects. The data also provide a rationale for the use of golden berries in the traditional medicine for various
ailments.
Keywords: Physalis peruviana L., Cape gooseberry, Flavonoids, Phenols, Phytochemicals.
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