TO STUDY THE IN VITRO INHIBITION OF BLOOD PLATELET AGGREGATION BY ISOLATED COMPOUNDS FROM CLAUSENA DENTATA (WILLD.) ROEM.
DOI:
https://doi.org/10.22159/ajpcr.2019.v12i12.35557Keywords:
Clausena dentata, Coumarin, Dentatin, Adenosine diphosphate, Aspirin, PlateletAbstract
Objectives: The objectives of the study were to study the antiplatelet aggregation activity of compounds isolated from extracts of Clausena dentata. Clausena (Rutaceae) is a genus of about 23 species of unarmed trees and shrubs. The stem bark of C. dentata is used in veterinary medicine for the treatment of wounds and sprains. Even though C. dentata has a lot of potential medical uses, the study of pharmacological properties is very scarce.
Methods: The plant C. dentata was collected from Kadagaman, near Tiruvannamalai, Tamil Nadu, India, and authenticated by Centre for Advanced Study in Botany, University of Madras, Chennai. The dry powder of stem bark was extracted with hexane, chloroform, and methanol. The extracts were subjected to qualitative phytochemical screening and column chromatography. Four compounds were isolated. All the isolated compounds were subjected to adenosine diphosphate (ADP)-induced platelet aggregation and compared with aspirin.
Results: The isolated compounds from C. dentata and standard aspirin showed significant antiplatelet activity against ADP-induced platelet aggregation.
Conclusion: The compounds, 3-(1,1-dimethylallyl)xanthyletin, dentatin, nordentatin, and carbazole showed significant antiplatelet aggregation activity. Among the compounds, nordentatin showed more activity of antiplatelet aggregation.
Downloads
References
Chopra RN, Nayar SL, Chopra IC. Glossary of Indian Medicinal Plants. New Delhi, India: C. S. I. R.; 1956. p. 246.
Kirtikar KR, Basu BD, Singh B. Indian Medicinal Plants. Dehradun, India: M/s. Bighensing Mahendra Palsingh Publishers; 1980. p. 2793.
Rao GS, Rao KS, Ravindranath B. Structure of diclausenan A and B. Tetrahedron Lett 1976;13:1019-20.
Gajalakshmi S, Vijayalakshmi S, Rajeswari VD. Phytochemical and pharmacological properties of Annona muricata: A review. Int J Pharm Pharm Sci 2012;4:3-6.
Sankaranarayanan S, Bama P, Ramachandran J, Jayasimman R, Kalaichelvan PT, Deccaraman M, et al. In vitro platelet aggregation inhibitory effect of triterpenoid compound from the leaf of Elephantopus scaber Linn. Int J Pharm Pharm Sci 2010;2:49-51.
O’Kennedy R, Thrones RD. Coumarins Biology, Applications and Mode of Action. Chickester, U.K: John Wiley and Sons Ltd.; 1997. p. 270.
Hoult JR, Payá M. Pharmacological and biochemical actions of simple coumarins: Natural products with therapeutic potential. Gen Pharmacol 1996;27:713-22.
Cooke D, Fitzpatrick B, O’Kennedy R, Mccormack T, Egan D. Coumarins Multifaceted Molecules with Many Analytical and other Applications O’Kennedy and Thornes, U.S.A. New York: John Wiley and Sons; 1997. p. 303-32.
Egan D, O’Kennedy R, Moran E, Cox D, Prosser E, Thornes RD, et al. The pharmacology, metabolism, analysis, and applications of coumarin and coumarin-related compounds. Drug Metab Rev 1990;22:503-29.
Harborne JB. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. London: Chapman and Hall Publishers; 1988. p. 278.
Harborne JB. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. London: Chapman and Hall Publishers; 1998. p. 293.
Subramaniam A, Sathyanarayana NM. Influence of certain dietary plant constituents on platelet aggregation. J Food Safety 1989;9:201-14.
Prentice CR. Platelets and atherosclerosis. Eur Heart J Suppl 1999;1:A3-7.
Meade TW, Vickers MV, Thompson SG, Stirling Y, Haines AP, Miller GJ, et al. Epidemiological characteristics of platelet aggregability. Br Med J (Clin Res Ed) 1985;290:428-32.
Collaborative overview of randomised trials of antiplatelet therapy I: Prevention of death, myocardial infarction, and stroke by prolonged antiplatelet therapy in various categories of patients. Antiplatelet trialists’ collaboration. BMJ 1994;308:81-106.
Hashizume T, Taniguchi M, Sato T, Fujii T. Arachidonic acid liberation induced by phosphatidic acid endogenously generated from membrane phospholipids in rabbit platelets. Biochim Biophys Acta 1994;1221:179-84.
Cimmniello C, Toschi. Atherothrombosis the role of platelets. Eur Heart J Suppl 1999;1:A8-13.
Patrono C. Aspirin as an antiplatelet drug. N Engl J Med 1994;330:1287-94.
Published
How to Cite
Issue
Section
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.
