PREPARATION AND INITIAL BIOCOMPATABILITY EVALUATION OF BIOGENIC HYDROXYAPATITE/ CHITOSAN/ PVA BIOCOMPOSITE AS A DRUG DELIVERY CARRIER OF PRONEUROGENIC FACTOR
Abstract
ÂObjective: This study explores the synthesis of biogenic hydroxyapatite (HAp)/chitosan (CS)/polyvinyl alcohol (PVA) bio-composites for delivery of
proneurogenic factor, retinoic acid for reconstruction of craniofacial deformities.
Methods: In order to accomplish this aim, we started with the synthesis of HAp using the biomolecules occluded in the cucumber peel (CPHAp).
Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction studies (XRD) studies confirmed the
purity and morphology of CPHAp. Further, a microenvironment for nerve cell growth was designed by synthesis of biogenic HAp/CS/PVA blends
loaded with retinoic acid (CS-PVA-CPHAp-all-trans-retinoic acid [ATRA]). The prepared biocomposites were characterized under advanced analytical
instruments such as SEM, FTIR, and XRD.
Results: The SEM analysis for the prepared biocomposites confirmed the formation of interconnected porous matrix. The results of FTIR confirm the
biocomposite formation without chemical modification of ATRA. From XRD the amorphous nature was confirmed, inducing suitability of the material
for delivery process. Release of ATRA from CS-PVA-CPHAp-ATRA was sustained, with a cumulative release of 55% at the end of 10th day. Furthermore,
3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay indicated that, the biocomposites are better in scaffold properties, and it
provides a healthier environment for cell attachment and spreading.
Conclusion: The porous CS-PVA CPHAp-ATRA composites may be applied to craniofacial tissue engineering as a long-term or permanent scaffold due
to their good biocompatibility and sustained release of proneurogenic factor.
Keywords: Hydroxyapatite/chitosan/polyvinyl alcohol bio-composites, Biocompatibility, Cell compatibility, Scaffold, Craniofacial deformities.
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