EFFECT OF STERCULIA QUADRIFIDA R. BR BARK TO IN VITRO DPPH RADICAL AND GLUTATHIONE PEROXIDASE ON DIABETIC WISTAR RATS

RAMBU KONDA A PRAING; TITIK SUNARNI

doi:10.22159/ajpcr.2020.v13i11.38805

Authors

RAMBU KONDA A PRAING Pharmaceutical Biology Research Group, Faculty of Pharmacy, Setia Budi University, Indonesia.
TITIK SUNARNI Pharmaceutical Biology Research Group, Faculty of Pharmacy, Setia Budi University, Indonesia.

DOI:

https://doi.org/10.22159/ajpcr.2020.v13i11.38805

Keywords:

Sterculia quadrifida RBr, 1,1-biphenyl-2-picryl-hydrazyl, Glutathione peroxidase, Antioxidant

Abstract

Objective: The aims of the research were to evaluate the antioxidant effect of Sterculia quadrifida R.Br bark by in vitro 1,1-biphenyl-2-picryl-hydrazyl (DPPH) radical scavenging method and glutathione peroxidase (GPx) activity on diabetic Wistar rats model.

Methods: S. quadrifida bark powder was extracted in ethanol 70% by maceration method. The antioxidant activity of ethanol extract was tested by DPPH radical scavenging method. The activity of GPx was evaluated from the liver of alloxan-induced diabetic rats. The antioxidant activity was determined by measuring the absorbance using ultraviolet-visible spectrophotometry method.

Results: The ethanol extract of S. quadrifida bark had a strong DPPH radical scavenging activity, with the IC50 value was 4.86±0.01 ug/ml, besides that the extract also showed significantly activity of GPx (p<0.05) at a dose of 65, 130, and 260 mg/kg bw.

Conclusion: The ethanol extract of S. quadrifida bark had a potent antioxidant activity by DPPH radical scavenging and GPx activity.

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