ANTIHYPERURICEMIC ACTIVITY OF FOUR PLANTS ANNONACEAE USING HYPERURICEMIC RATS MODEL AND ENZYME ASSAY
Objective: The aim of the research is to study the antihyperuricemic activity of Annona muricata L, Annona squamosa L, Annona reticulata L, and
Stelechocarpus burahol (Bl.) Hook. F. & Th. (Annonaceae).
Methods: The dried powdered leaves were extracted using ethanol by maceration method. The ethanolic extracts of four plants were administered
orally to potassium oxonate-induced rats. Serum samples were collected from rats at the 1
hr after drug administration. Enzyme assay was done
by xanthine oxidase (XO). The XO inhibitory activity was measured by UV-Vis spectrophotometry, and the degree of enzyme inhibition was determined
by measuring the absorbance of uric acid formation.
Results: The result of this study revealed that all of the ethanolic extracts had a significant effect on hyperuricemic rats, and S. burahol extract
decreased uric acid level was equal to level normal control. However, the antihyperuricemic activity was not parallel with XO inhibitor activity which
showed weak activity with IC
of A. reticulata was 171.73 Âµg/ml, while IC
of A. muricata, A. squamosa, and S. burahol more than 200 Âµg/ml.
Conclusion: The ethanolic extract of S. burahol was more potential than three other extracts in decreasing in uric acid levels. The results scientifically
confirmed that S. burahol can be developed as potential antihyperuricemic agents.
Keywords: Antihyperuricemia, Annona muricata, Annona squamosa, Annona reticulata, Stelechocarpus burahol, Potassium oxonate, Xanthine oxidase.
Yu KH. Febuxostat: A novel non-purine selective inhibitor of xanthine
oxidase for the treatment of hyperuricemia in gout. Recent Pat Inflamm
Allergy Drug Discov 2007;1(1):69-75.
So A, Thorens B. Uric acid transport and disease. J Clin Invest
Preitner F, Bonny O, LaverriÃ¨re A, Rotman S, Firsov D, Da Costa A,
et al. Glut9 is a major regulator of urate homeostasis and its genetic
inactivation induces hyperuricosuria and urate nephropathy. Proc Natl
Acad Sci U S A 2009;106(36):15501-6.
Jin M, Yang F, Yang I, Yin Y, Luo JJ, Wang H, et al. Uric acid,
hyperuricemia and vascular diseases. Front Biosci (Landmark Ed)
Choi HK, Mount DB, Reginato AM; American College of Physicians;
American Physiological Society. Pathogenesis of gout. Ann Intern Med
Wang SY, Yang CW, Liao JW, Zhen WW, Chu FH, Chang ST. Essential
oil from leaves of Cinnamomum osmophloeum acts as a xanthine
oxidase inhibitor and reduces the serum uric acid levels in oxonateinduced
mice. Phytomedicine 2008;15(11):940-5.
Masuoka N, Nihei K, Masuoka T, Kuroda K, Sasaki K, Kubo I.
The inhibition of uric acid formation catalyzed by xanthine
oxidase properties of the alkyl caffeates and cardol. J Food Res
Pacher P, Nivorozhkin A, SzabÃ³ C. Therapeutic effects of xanthine
oxidase inhibitors: Renaissance half a century after the discovery of
allopurinol. Pharmacol Rev 2006;58(1):87-114.
Fagugli RM, Gentile G, Ferrara G, Brugnano R. Acute renal and
hepatic failure associated with allopurinol treatment. Clin Nephrol
de Sousa OV, Vieira GD, de Jesus RG. de Pinho J, Yamamoto CH,
Alves MS. Antinociceptive and anti-inflammatory activities of the
ethanol extract of Annona muricata L. leaves in animal models. Int J
Mol Sci 2010;11(5):2067-78.
Kumar AS, Venkatarathanamma V, Saibabu VN, Ram KS. Antipyretic
activity of Annona plants leaves on brewerâ€™s yeast induced febrile rats.
Asian J Pharm Clin Res 2015;8(3):210-2.
Adewole SO, Ojewole JH. Protective effects of Annona muricata
Linn. (Annonaceae) leaf aqueous extract on serum lipid profiles and
oxidative stress in hepatocytes of Streptozotocin-treated diabetic rats.
Afr J Trad CAM 2009;6(1):30-41.
Liaw CC, Chang FR, Lin CY, Chou CJ, Chiu HF, Wu MJ, et al. New
cytotoxic monotetrahydrofuran annonaceous acetogenins from Annona
muricata. J Nat Prod 2002;65(4):470-5.
Sri-Wahjuni S, Putra-Manuaba IB, Rahayu-Artini NP, Wahyu-Dwijani S.
Uric acid inhibition activity of Annona muricata L. leave extract in
hyperuricemia induced wistar rat. Adv Pure Appl Chem 2012;2(1):8690.
Gupta RK, Kesari AN, Diwakar S, Tyagi A, Tandon V, Chandra R, et al.
In vivo evaluation of anti-oxidant and anti-lipidimic potential of Annona
squamosa aqueous extract in Type 2 diabetic models. J Ethnopharmacol
Soni VK, Yadav DK, Bano N, Dixit P, Pathak M, Maurya R, et al.
N-Methyl-6, 7-dimethoxyisoquinolone in Annona squamosa twigs is
the major immune modifier to elicit polarized Th1 immune response in
BALB/c mice. Fitoterapia 2012;83(1):110-6.
Tomar RS, Sisodia SS. Antidiabetic activity of Annona squamosa L. in
experimental induced rats. Int J Pharm Biol Arch 2012;3(6):1492-5.
Rajini R, Nisha JM. Potential bioactivity of Annona reticulata L. leaf
extract and its compound identification by GC-MS. Int J Sci Innov
Thang TD, Kuo PC, Huang GJ, Hung NH, Huang BS, Yang ML, et al.
Chemical constituents from the leaves of Annona reticulata and their
inhibitory effects on NO production. Molecules 2013;18(4):4477-86.
Sunarni T, Pramono S, Asmah R. Antioxidant free radical scavenging
of flavonoid from the leaves of Stelechocarpus burahol (Bl.) Hook f. &
Th. Indonesian J Pharm 2007;8(3):111-6.
Sutomo. Decrease of uric acid degree on broiller cock hyperuricaemia
by ether petroleum fraction of kepel leaves (Stelechocarpus burahol
Hook.). Sains dan Terapan Kimia 2008;2(1):14-22.
Purwantiningsih, Hakim AR, Purwantini I. Anti-hyperuricemic activity
of the kepel (Stelechocarpus burahol (Bl.) Hook. F. & Th.) leaves
extract and xanthine oxidase inhibitory study. Int J Pharm Pharm Sci
Liu X, Chen R, Shang Y, Jiao B, Huang C. Lithospermic acid as a novel
xanthine oxidase inhibitor has anti-inflammatory and hypouricemic
effects in rats. Chem Biol Interact 2008;176(2-3):137-42.
Abdullahi A, Hamzah RU, Jigam AA, Yahya A, Kabiru AY,
Muhammad H, et al.. Inhibitory activity of xanthine oxidase by
fractions Crateva adansonii. J Acute Dis 2012;1(2):126-9.
Hu QH, Zhu JX, Ji J, Wei LL, Miao MX, Ji H. Fructus gardenia extract
ameliorates oxonate-induced hyperuricemia with renal dysfunction in
mice by regulating organic ion transporters and mOIT3. Molecules
McGill NW. Review: The epidemiology and treatment of gout.
Rheumatol Res Rev 2011;3:73-82.
Reinders MK, Jansen TL. Management of hyperuricemia in gout:
Focus on febuxostat. Clin Interv Aging 2010;5:7-18.
Nagao A, Seki M, Kobayashi H. Inhibition of xanthine oxidase by
flavonoids. Biosci Biotechnol Biochem 1999;63(10):1787-90.
Nguyen MT, Awale S, Tezuka Y, Shi L, Zaidi SF, Ueda JY, et al.
Hypouricemic effects of acacetin and 4,5-o-dicaffeoylquinic acid
methyl ester on serum uric acid levels in potassium oxonate-pretreated
rats. Biol Pharm Bull 2005;28(12):2231-4.
Mo SF, Zhou F, Lv YZ, Hu QH, Zhang DM, Kong LD. Hypouricemic
action of selected flavonoids in mice: Structure-activity relationships.
Biol Pharm Bull 2007;30(8):1551-6.
Purwantini P, Santoso D. Identification of standard parameters of kepel
leaves (Stelechocarpus burahol (Bl.) Hook. F. & Th.) and the extract as
raw material for anti-hyperuricemic medicaments. Asian J Pharm Clin
Chavan MJ, Wakte PS, Shinde DB. Analgesic and anti-inflammatory
activity of caryophyllene oxide from Annona squamosa L. bark.
Reddy SK, Reddy CS, Ganapaty S. Analgesic and anti-inflammatory
activity of stem bark of Annona reticulata Linn. J Chem Pharm Sci
Cos P, Ying L, Calomme M, Hu JP, Cimanga K, Van Poel B, et al.
Structure-activity relationship and classification of flavonoids as
inhibitors of xanthine oxidase and superoxide scavengers. J Nat Prod
Chen CY, Huang CC, Tsai KC, Huang WJ, Huang WC, Hsu YC, et al.
Evaluation of the Antihyperuricemic Activity of Phytochemicals from
Davallia formosana by Enzyme Assay and Hyperuricemic Mice Model.
Evid Based Complement Alternat Med 2014;2014:873607.
Huang J, Wang S, Zhu M, Chen J, Zhu X. Effects of genistein, apigenin,
quercetin, rutin and astilbin on serum uric acid levels and xanthine
oxidase activities in normal and hyperuricemic mice. Food Chem
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