INVITRO ANTIOXIDANT ACTIVITIES OF VARIOUS LEAVES EXTRACTS FROM FIVE VARIETIES OF RAMBUTAN (Nephelium lappaceum) AND ITS CORRELATION WITH TOTAL FLAVONOID, PHENOLIC, CAROTENOID CONTENT
Abstract
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Objectives: The objectives of this research were to study antioxidant activity from different polarities extracts of rambutan (Nephelium lappaceum) leaves
using two methods of antioxidant testing which were 2-2-diphenyl-1-picrylhydrazyl (DPPH) and 2-2'-azinobis (3-ethyl-benzothiazoline-6-sulfonic acid)
(ABTS) and correlation of total flavonoid, phenolic and carotenoid content in various extracts of rambutan leaves with DPPH and ABTS antioxidant activities.
Methods: Extraction was performed by reflux using different polarity solvents. The extracts were evaporated using rotavapor. Antioxidant activities
using DPPH and ABTS assays, determination of total phenolic, flavonoid and carotenoid content were conducted by spectrophotometry ultra violetvisible
and its correlation with DPPH and ABTS antioxidant capacities were analyzed by Pearson's method.
Results: RPH3 (ethanolic leaves extract of rapiah rambutan) had the highest DPPH scavenging capacity with inhibitory concentration 50 (IC50) 14.666 μg/mL
and the highest ABTS scavenging activity with IC50 12.826 μg/mL. RPH3 had the highest phenolic content (29.46 g gallic acid equivalents/100 g), NON2
(ethyl acetate leaves extract of non-consumption rambutan) contained the highest total flavonoid (9.59 g quercetin equivalents/100 g), and NON1
(n-hexane leaves extract of non-consumption rambutan) had the highest carotenoid 10.99 g beta-carotene equivalent/100 g.
Conclusions: There were positively and high correlation between total phenolic content in all of the leaves extracts with their antioxidant activity
using DPPH and ABTS assays. DPPH scavenging activities in all of the samples gave linear result with ABTS scavenging capacities.
Keywords: Antioxidants, 2-2-diphenyl-1-picrylhydrazyl, 2-2'-azinobis (3-ethyl-benzothiazoline-6-sulfonic acid), Rambutan leaves, Flavonoid,
Phenolic, Carotenoid.
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Thaipong K, Boonprakob U, Crosby K, Zevallos LC, Byrne DH. Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. J Food Compost Anal 2006;19:669-75.
Boudjou S, Oomah BD, Zaidi F, Hosseinian F. Phenolics content and antioxidant and anti-inflammatory activities of legume fractions. Food Chem 2013;138(2-3):1543-50.
Khatua S, Roy T, Acharya K. Antioxidant and free radical scavenging capacity of phenolic extract from Russula laurocerasi. Asian J Pharm Clin Res 2013;6(4):156-60.
Thitilertdecha N, Teerawutgulrag A, Rakariyatham N. Antioxidant and antibacterial activities of Nephelium lappaceum L. extracts. Food Sci Technol 2008;41:2029-35.
Xu BJ, Chang SK. A comparative study on phenolic profiles and antioxidant activities of legumes as affected by extraction solvents. J Food Sci 2007;72(2):S159-66.
Xu BJ, Chang SK. Total phenolic content and antioxidant properties of eclipse black beans (Phaseolus vulgaris L.) as affected by processing methods. J Food Sci 2008;73(2):H19-27.
Abirami MS, Muthuswamy. Antioxidant potential, total phenolic and total flavonoids content of various extracts from whole plant of Polycarpaea corymbosa Lam. Asian J Pharm Clin Res 2013;6(4):121‑4.
Samuagam L, Sia CM, Akowuah GA, Okechukwu PN, Yim HS. The effect of extraction conditions on total phenolic content and free radical scavenging capacity of selected tropical fruits’ peel. Health Environ J 2013;4(2):80-102.
Thitilertdecha N, Teerawutgulrag A, Kilburn JD, Rakariyatham N. Identification of major phenolic compounds from Nephelium lappaceum L. and their antioxidant activities. Molecules 2010;15(3):1453-65.
Ling LT, Palanisamy UD. Review: Potential antioxidants from tropical plants. In: Valdez B, editor. Food Industrial Processes-Methods. Kuala Lumpur: In Tech; 1999. p. 64-72.
Lindley MG. The impact of food processing on antioxidants in vegetable oils, fruits and vegetables. Trends Food Sci Technol 1998;9:336-40.
Souri E, Amin G, Farsan H, Barazandeh TM. Screening antioxidant activity and phenolic content of 24 medicinal plats extracts. DARU J Pharm Sci 2008;16:83-7.
Müller L, Fröhlich K, Böhm V. Comparative antioxidant activities of carotenoids measured by ferric reducing antioxidant power (FRAP), ABTS bleaching assay (aTEAC), DPPH assay and peroxyl radical scavenging assay. Food Chem 2011;129:139-48.
Tachakittirungrod S, Okonogi S, Chowwanapoonpohn S. Study on antioxidant activity of certain plants in Thailand: Mechanism of antioxidant action of guava leaf extract. Food Chem 2007;103:381-8.
Molyneux P. The use of stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. J Sci Technol 2003;26(2):211-9.
Blois MS. Antioxidant determination by the use of stable free radicals. Nature 1958;181:1199-2000.
Bedawey AA. Characteristics of antioxidant isolated from some plants sources. Cairo: Shibin El-Kom; 2010. p. 1-11.
Li XC, Wang XZ, Chen DF, Chen SZ. Antioxidant activity and mechanism of protochatechuic acid in vitro. J Funct Food Health Dis 2011;1:232-44.
Pellegrini N, Serafini M, Colombi B, Rio DD, Salvatore S. Total antioxidant capacity of plant food, beverages and oils consumed in Italy assessed by three different in vitro assays. J Am Coll Nutr 2003;133:2812-9.
Pourmorad F, Hosseinimehr SJ, Shahabimajd N. Antioxidant activity, phenol and flavonoid content of some selected Iranian medicinal plants. Afr J Biotechnol 2006;5(11):1142-5.
Chang CC, Yang MH, Wen HM, Chern JC. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal 2002;10:178-82.
Apak R, Güçlü K, Demirata B, Ozyürek M, Celik SE, Bektasoglu B, et al. Comparative evaluation of various total antioxidant capacity assays applied to phenolic compounds with the CUPRAC assay. Molecules 2007;12(7):1496-547.
Heim KE, Tagliaferro AR, Bobilya DJ. Flavonoid antioxidants: chemistry, metabolism and structure-activity relationships. J Nutr Biochem 2002;13(10):572-84.
Foote CS. Free Radicals in Biology. 3rd ed. New York: Academic Press; 1976.
Beutner S, Bloedorn B, Hoffmann T, Martin HD. Synthetic singlet oxygen quenchers. Methods Enzymol 2000;319:226-41.
Kobayashi M, Sakamoto Y. Singlet oxygen quenching ability of astaxanthin esters from the green alga Haematococcus pluvialis. Biotechnol Lett 1999;21:265-9.
Charles DJ. Antioxidant Properties of Spices Leaves and Other. London: John Willey; 2013.
Huang D, Ou B, Prior RL. The chemistry behind antioxidant capacity assays. J Agric Food Chem 2005;53:1841-56.
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