ANALYSIS OF OXIDATIVE STRESS MARKERS IN CHRONIC CONSUMPTION OF MONOSODIUM GLUTAMATE ON LIVER OF WISTAR ALBINO RATS

SURENDRA BABU THANGACHI; VARSHA SRIRAM MOKHASI; AGA AMMAR MURTHUZA

doi:10.22159/ajpcr.2021.v14i11.43103

Authors

SURENDRA BABU THANGACHI Department of Anatomy, Vydehi Institute of Medical Sciences and Research Centre, Bengaluru, Karnataka, India.
VARSHA SRIRAM MOKHASI Department of Anatomy, Vydehi Institute of Medical Sciences and Research Centre, Bengaluru, Karnataka, India.
AGA AMMAR MURTHUZA Department of Anatomy, Vydehi Institute of Medical Sciences and Research Centre, Bengaluru, Karnataka, India.

DOI:

https://doi.org/10.22159/ajpcr.2021.v14i11.43103

Keywords:

Monosodium glutamate, Antioxidant, Oxidative stress, Reactive oxygen species

Abstract

Objective: The study was intended to explore whether Monosodium glutamate (MSG) induces oxidative stress on the liver of Wistar albino rats when fed chronically at three different doses, namely, low, mid, and high doses identical to human consumption doses in growing countries.

Methods: The acclimatized Wistar albino rats (n=24) were randomly selected and grouped into four groups, namely Control, Low dose MSG (180 mg kg), Mid dose MSG (360 mg/kg), and High dose MSG (720 mg/kg). The animals were orally administered MSG for 120 days. After completion of the experimental period (120 days), euthanized animal liver was homogenized to investigate the oxidative stress marker enzymes such as Superoxide Dismutase (SOD), Glutathione Peroxidase (GPx), Catalase (CAT), and Myeloperoxidase (MPO).

Results: The MPO showed a significant increase (p<0.05) in liver homogenate of all MSG induced groups when compared to control group. The SOD, CAT, and GPx activity deteriorated (p<0.05) in monosodium induced groups contrasting to the control group.

Conclusion: The effects of MSG on oxidative stress markers on liver homogenate in the current study exhibited erratic abnormal changes in oxidative stress markers of monosodium induced groups which contemplate the harmful effects of MSG consumed chronically. The further studies should confirm the genetic basis of oxidative stress damage and transform the safety regulations of MSG consumption throughout the world.

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References

Sano C. History of glutamate production. Am J Clin Nutr 2009;90:728-32. DOI: https://doi.org/10.3945/ajcn.2009.27462F

Win DT. MSG-flavor enhancer or deadly killer. AU J Technol 2008;12:43-9.

Sharma V, Deshmukk RA. A fifth taste or bio bomb. Eur J Pharma Med Res 2015;2:381-400.

Henry-Unaeze HN. Update on food safety of monosodium l-glutamate (MSG). Pathophysiology 2017;24:243-9. DOI: https://doi.org/10.1016/j.pathophys.2017.08.001

Kazmi Z, Fatima I, Perveen S, Malik SS. Monosodium glutamate: Review on clinical reports. Int J Food Prop 2017;20:1-9. DOI: https://doi.org/10.1080/10942912.2017.1295260

Datta A, Hossain A, Roy S. An overview on monosodium glutamate: Its direct and indirect effects. Res J Pharm Technol 2019;12:6187-92. DOI: https://doi.org/10.5958/0974-360X.2019.01074.6

Sies H. What is oxidative stress? In: Developments in Cardiovascular Medicine. Boston, MA: Springer; 2000. p. 1-8. DOI: https://doi.org/10.1007/978-1-4615-4649-8_1

Yoshikawa T, Naito Y. What is oxidative stress? Jap Med Assoc J 2002;45:271-6.

Arauz J, Ramos-Tovar E, Muriel P. Redox state and methods to evaluate oxidative stress in liver damage: From bench to bedside. Ann Hepatol 2016;15:160-73.

Farombi EO, Onyema OO. Monosodium glutamate-induced oxidative damage and genotoxicity in the rat: Modulatory role of Vitamin C, Vitamin E and quercetin. Hum Exp Toxicol 2006;25:251-9. DOI: https://doi.org/10.1191/0960327106ht621oa

Eid RA, Al-Shraim M, Zaki MS, Kamar SS, Abdel Latif NS, Negm S, et al. Vitamin E protects against monosodium glutamate-induced acute liver injury and hepatocyte ultrastructural alterations in rats. Ultrastruct Pathol 2019;43:199-208. DOI: https://doi.org/10.1080/01913123.2019.1673860

Zanfirescu A, Ungurianu A, Tsatsakis AM, Nițulescu GM, Kouretas D, Veskoukis A, et al. A review of the alleged health hazards of monosodium glutamate. Compr Rev Food Sci Food Saf 2019;18:1111-34. DOI: https://doi.org/10.1111/1541-4337.12448

Lestarisa T, Alexandra FD, Jelita H, Suhartono E. Myeloperoxidase as an indicator of liver cells inflammation induced by mercury. Int J Pharm Clin Res 2016;15:1516-21.

Del Carmen Contini M, Millen N, Riera L, Mahieu S. Kidney and liver functions and stress oxidative markers of monosodium glutamate-induced obese rats. Food Public Health 2012;2:168-77. DOI: https://doi.org/10.5923/j.fph.20120205.08

Zhu R, Wang Y, Zhang L, Guo Q. Oxidative stress and liver disease: Oxidative stress and liver disease. Hepatol Res 2012;42:741-9. DOI: https://doi.org/10.1111/j.1872-034X.2012.00996.x

Albrahim T, Binobead MA. Roles of Moringa oleifera leaf extract in improving the impact of high dietary intake of monosodium glutamate-induced liver toxicity, oxidative stress, genotoxicity, DNA damage, and PCNA alterations in male rats. Oxid Med Cell Longev 2018;2018:4501097. DOI: https://doi.org/10.1155/2018/4501097

Li S, Tan HY, Wang N, Zhang ZJ, Lao L, Wong CW, et al. The role of oxidative stress and antioxidants in liver diseases. Int J Mol Sci 2015;16:26087-124. DOI: https://doi.org/10.3390/ijms161125942

Naito Y, Lee MC, Kato Y, Nagai R, Yonei Y. Oxidative stress markers. Anti Aging Med 2010;7:36-44. DOI: https://doi.org/10.3793/jaam.7.36

Hareeri NA, Alrasheedi AA, Eassaw MM. Effect of sesame on liver enzymes and lipid profile inrats exposed to oxidative stress induced by monosodium glutamate. J Am Sci 2017;13:71-8.

Chakraborty SP. Patho-physiological and toxicological aspects of monosodium glutamate. Toxicol Mech Methods 2019;29:389-96. DOI: https://doi.org/10.1080/15376516.2018.1528649

Okwudiri O. Monosodium glutamate induces oxidative stress and affects glucose metabolism in the kidney of rats. Int J Biochem Res Rev 2012;2:1-11. DOI: https://doi.org/10.9734/IJBCRR/2012/827

Cichoż-Lach H, Michalak A. Oxidative stress as a crucial factor in liver diseases. World J Gastroenterol 2014;20:8082-91. DOI: https://doi.org/10.3748/wjg.v20.i25.8082

Pavlovic V, Pavlovic D, Kocic G, Sokolovic D, Jevtovic-Stoimenov T,Cekic S, et al. Effect of monosodium glutamate on oxidative stress and apoptosis in rat thymus. Mol Cell Biochem 2007;303:161-6. DOI: https://doi.org/10.1007/s11010-007-9469-7

Quines CB, Chagas PM, Hartmann D, Carvalho NR, Soares FA, Nogueira CW. (p-ClPhSe)2 reduces hepatotoxicity induced by monosodium glutamate by improving mitochondrial function in rats: (P-ClPhSe)2 reduces hepatotoxicity induced by MSG. J Cell Biochem 2017;118:2877-86. DOI: https://doi.org/10.1002/jcb.25938