EVALUATION OF ACUTE AND SUB-ACUTE TOXICITY OF METHANOLIC LEAF EXTRACT OF BAMBUSA VULGARIS ON MALE WISTAR RATS

SUSMITA  SARKAR; PANISREE ROY CHOWDHURY; DIPAYAN CHOUDHURI

doi:10.22159/ajpcr.2024.v17i5.50325

Authors

SUSMITA SARKAR Department of Human Physiology, Endocrinology and Reproductive Physiology Research Laboratory, Tripura University (A Central University), Suryamaninagar, Agartala, Tripura, India. https://orcid.org/0009-0006-8649-6837
PANISREE ROY CHOWDHURY Department of Human Physiology, Endocrinology and Reproductive Physiology Research Laboratory, Tripura University (A Central University), Suryamaninagar, Agartala, Tripura, India. https://orcid.org/0009-0007-9445-0262
DIPAYAN CHOUDHURI Department of Human Physiology, Tripura University (A Central University), Suryamaninagar, Agartala, Tripura, India https://orcid.org/0000-0001-8516-0211

DOI:

https://doi.org/10.22159/ajpcr.2024.v17i5.50325

Keywords:

Bambusa vulgaris, Methanolic extract, Acute toxicity,, Sub-acute toxicity, LD50 dose

Abstract

Objectives: The study aims to evaluate the potential toxicity of methanolic extract of leaves of Bambusa vulgaris in male Wistar rats.

Methods: The methanolic extract was prepared for acute and sub-acute toxicity to determine the lethal dose. During the acute toxicity study, male rats were orally administered with leaf extracts at single doses of 100, 500,800, and 2000 mg/kg according to OECD Guidelines 425 for 14 days. Sub-acute toxicity dose at 200, 400, and 800 mg/kg was studied by daily dosing on male rats for 30 days.

Results: The acute toxicity study revealed no lethal effects and behavioral signs of toxicity at the tested doses indicating that LD50 is <2000 mg/kg. In a sub-acute study, non-significant changes in the body weight (p<0.05), body weight gain percentage (p<0.05) food intake (p<0.05), and relative organ weight percentage (p<0.05), and hematological parameters (p<0.05) of the rats were observed. A significant (p<0.05) increase in ALT level was observed dose at 800 mg/kg. Histopathological analysis showed mild cell distortion in the liver and kidney in male rats treated at 800 mg/kg of extract.

Conclusion: Our study suggests that oral administration of methanolic leaf extract Bambusa vulgais is safe. However, a dose of 800 mg/kg may be toxic for the liver and kidney.

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