• Talha Jawaid
  • Syed Aman Imam
  • Mehnaz Kamal Faculty of Pharmacy, Integral University, Dasauli, Kursi Road, Lucknow, Uttar Pradesh - 226 026, India


Objective: The objective was to investigate the antidepressant activity of methanolic extract of leaves of Verbena officinalis Linn. (MEVO) in mice.
Methods: The present study evaluates the antidepressant activity of MEVO in mice using the tail suspension test (TST) and forced swimming test
(FST). Their influence on spontaneous locomotor activity (SLMA) was also studied in mice. The MEVO (100 mg/kg, p.o. and 200 mg/kg, p.o.) was
administered orally in TST, FST and SLMA for 7 successive days in separate groups of Swiss mice.
Results: The extract at 100 and 200 mg/kg, p.o. were able to decrease the immobility time of mice doseâ€dependently when subjected to both tail
suspension and forced swim tests and the effects are comparable to that of control group. These results demonstrated that MEVO had specifically
antidepressant effects.
Conclusion: The present study suggested that MEVO possessed potential antidepressant effects which could be of therapeutic interest for using in
the treatment of patients with depression.
Keywords: Verbena officinalis Linn., Antidepressant activity, Forced swim test, Tail suspension test, Spontaneous locomotor activity.


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Author Biography

Mehnaz Kamal, Faculty of Pharmacy, Integral University, Dasauli, Kursi Road, Lucknow, Uttar Pradesh - 226 026, India

Faculty of Pharmacy, Integral University, Dasauli, Kursi Road, Lucknow, Uttar Pradesh - 226 026, India



Meti V, Ruckmani A, Chandrashekhar K, Konda VG, Madhavi E, et al. Antidepressant activity of ethanolic extract of Piper betle leaves in mice. Current Res Neurosci 2012;2:11-6.

Santosh P, Venugopal R, Nilakash AS, Kunjbihari S, Mangala L. Antidepressant activity of methanolic extract of Passiflora foetida leaves in mice. Int J Pharm Pharm Sci 2011;3(1):112-5.

Sharma VK, Chauhan NS, Lodhi SR, Singhai AK. Antidepressant activity of Zizyphus xylopyrus. Int J Phytomed 2009;1:12-7.

Holtzheimer PE 3rd, Nemeroff CB. Future prospects in depression research. Dialogues Clin Neurosci 2006;8(2):175-89.

Deepak M, Handa SS. Antiinflammatory activity and chemical composition of extracts of Verbena officinalis. Phytother Res 2000;14(6):463-5.

Hernández NE, Tereschuk ML, Abdala LR. Antimicrobial activity of flavonoids in medicinal plants from Tafí del Valle (Tucumán, Argentina). J Ethnopharmacol 2000;73(1-2):317-22.

Lai SW, Yu MS, Yuen WH, Chang RC. Novel neuroprotective effects of the aqueous extracts from Verbena officinalis Linn. Neuropharmacology 2006;50(6):641-50.

Calvo MI. Anti-inflammatory and analgesic activity of the topical preparation of Verbena officinalis L. J Ethnopharmacol 2006;107(3):380-2.

Casanova E, García-Mina JM, Calvo MI. Antioxidant and antifungal activity of Verbena officinalis L. leaves. Plant Foods Hum Nutr 2008;63(3):93-7.

Rehecho S, Hidalgo O, Garcia-Iniguez de Cirano M, Navarro I, Astiasaran I, Ansorena D, et al. Chemical composition, mineral content and antioxidant activity of Verbena officinalis L. LWT Food Sci Tech 2011;44:875-82.

Khare CP. Indian Medicinal Plants: An Illustrated Dictionary. Berlin, Heidelberg: Springer Verlag; 2007. p. 669-71.

Pemminati S, Gopalakrishna HN, Shenoy AK, Sahu SS, Mishra S, Meti V, et al. Antidepressant activity of aqueous extract of fruits of Emblica officinalis in mice. Int J Appl Bio Pharm Tech 2010;1(2):448‑54.

Dhingra D, Sharma A. Evaluation of antidepressant-like activity of glycyrrhizin in mice. Int J Pharmacol 2005;37(6):390-4.

Rivera F, Gervaz E, Sere C, Dajas F. Toxicological studies of the aqueous extract from Achyrocline satureioides (Lam.) DC (Marcela). J Ethnopharmacol 2004;95(2-3):359-62.

Steru L, Chermat R, Thierry B, Simon P. The tail suspension test: A new method for screening antidepressants in mice. Psychopharmacology (Berl) 1985;85(3):367-70.

Porsolt RD, Bertin A, Jalfre M. Behavioral despair in mice: A primary screening test for antidepressants. Arch Int Pharmacodyn Ther 1977;229(2):327-36.

Sanmukhani J, Anovadiya A, Tripathi CB. Evaluation of antidepressant like activity of Curcumin and its combination with fluoxetine and imipramine: An acute and chronic study. Acta Pol Pharm 2011;68(5):769-75.

Hasrat JA, De Bruyne T, De Backer JP, Vauquelin G, Vlietinck AJ. Isoquinoline derivatives isolated from the fruit of Annona muricata as 5-HTergic 5-HT1A receptor agonists in rats: Unexploited antidepressive (lead) products. J Pharm Pharmacol 1997;49(11):1145-9.

Hasrat JA, Pieters L, De Backer JP, Vauquelin G, Vlietinck AJ. Screening of medicinal plants from Suriname for 5-HT (1A) ligands: Bioactive isoquinoline alkaloids from the fruit of Annona muricata. Phytomedicine 1997;4(2):133-40.

Rénéric JP, Lucki I. Antidepressant behavioral effects by dual inhibition of monoamine reuptake in the rat forced swimming test. Psychopharmacology (Berl) 1998;136(2):190-7.

Sapolsky RM. The possibility of neurotoxicity in the hippocampus in major depression: A primer on neuron death. Biol Psychiatry 2000;48(8):755-65.

Rajkowska G, Miguel-Hidalgo JJ, Wei J, Dilley G, Pittman SD, Meltzer HY, et al. Morphometric evidence for neuronal and glial prefrontal cell pathology in major depression. Biol Psychiatry 1999;45(9):1085-98.



How to Cite

Jawaid, T., S. Aman Imam, and M. Kamal. “ANTIDEPRESSANT ACTIVITY OF METHANOLIC EXTRACT OF VERBENA OFFICINALIS LINN. PLANT IN MICE”. Asian Journal of Pharmaceutical and Clinical Research, vol. 8, no. 4, July 2015, pp. 308-10,



Original Article(s)