ANTIDIABETIC AND ANTIHYPERLIPIDEMIC EFFCETS OF THE ETHANOLIC EXTRACT OF ALOCASIA INDICA RHIZOMES IN HIGH FAT DIET/STREPTOZOTOCIN AND STREPTOZOTOCIN/NICOTINAMIDE-INDUCED TYPE 2 DIABETIC RATS
Abstract
Objective: The investigate the antidiabetic and antihyperlipidemic effect of ethanolic extract of Alocasia indica (EEAI) rhizomes in high-fat
diet/streptozotocin (HFD/STZ) and STZ/nicotinamide-induced Type 2 diabetic rats.
Methods: Diabetes was induced in male Wistar rats by the administration of a HFD for 15 days/STZ (35 mg/kg b.w., i.p.) and STZ (60 mg/kg b.w.,
i.p.)/nicotinamide (110 mg/kg b.w., i.p.). EEAI (100 and 200 mg/kg b.w., p.o.) was administered to diabetic rats for 28 days in HFD/STZ-induced
Type 2 diabetic rats and for 15 days in STZ/nicotinamide-induced Type 2 diabetic rats. The effect of EEAI on blood glucose and body weight was
studied in Type 2 diabetic rats. All these effects were compared with glibenclamide (5 mg/kg b.w., p.o.) as a reference antidiabetic drug.
Results: The administration of the EEAI (100 and 200 mg/kg b.w., p.o.) resulted in a significant decrease in blood glucose level and significant increase
in body weight in the HFD/STZ and STZ/nicotinamide-induced Type 2 diabetic rats. Further EEAI showed antihyperlipidemic activity as evidenced
by significant decrease in serum total cholesterol, triglycerides, low-density lipoprotein cholesterol (LDL-C), very LDL-C levels coupled together with
elevation of high-density lipoprotein cholesterol level in diabetic rats in the HFD/STZ and STZ/nicotinamide-induced Type 2 diabetic rats.
Conclusion: The results suggest that the EEAI rhizomes possess a promising effect on the HFD/STZ and STZ/nicotinamide-induced Type 2 diabetes.
Keywords: Antidiabetic, Antihyperlipidemic, Alocasia indica, High-fat diet, Streptozotocin, Nicotinamide.
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References
Christudas S, Savarimuthu I, Paul A. Antidiabetic effect of
Symplocos cochichinensis (Lour.) S. Moore in type 2 diabetic rats.
J Ethnopharmacol 2011;134(2):298-304.
Sharma V, Kumar S, Patel H. Hypoglycemic activity of Ficus Glomerata
in alloxan induced diabetic rats. Indian J Pharmacol 2010;1:18-22.
Treated group
(EEAI 100 mg/kg b.w.)
Treated group
(EEAI 200 mg/kg b.w.)
TC (mg/dl) 92.50±6.56 186.25±7.66 103.22±5.62 99.74±5.28 94.64±4.88
TG (mg/dl) 64.40±4.73 198.36±7.28 92.60±5.82 78.86±4.26** 72.27±4.16
HDL-C (mg/dl) 42.58±1.32 26.20±0.86 32.02±0.71* 36.84±1.16 40.28±1.76**
LDL-C (mg/dl) 37.04±1.22 120.38±6.76 52.68±4.75** 42.87±2.75 39.91±1.63**
VLDL-C (mg/dl) 12.88±0.94 39.67±1.45 18.52±1.16 15.77±0.85 14.45±0.83
Each value is mean±SEM of 6 rats in each group. *p<0.05, **p<0.01 and ***p<0.001 in comparison to diabetic control. EEAI: Ethanolic extract of Alocasia indica,
TG: Triglyceride, HDL-C: High-density lipoprotein cholesterol, LDL-C: Low-density lipoprotein cholesterol, VLDL-C: Very low-density lipoprotein cholesterol, High fat
diet, STZ: Streptozotocin, SEM: Standard error of mean
Table 7: Effect of EEAI on TC, TG, HDL-C, LDL-C and VLDL-C levels in STZ/nicotinamide-induced Type 2 diabetic rats
Lipid level Control Diabetic
control
Treated group
(EEAI 100 mg/kg b.w.)
Treated group
(EEAI 200 mg/kg b.w.)
TC (mg/dl) 71.60±5.68 166.20±6.66 97.20±5.73 94.47±5.68 83.64±4.68
TG (mg/dl) 74.40±5.89 176.60±6.87 102.60±6.87 91.82±5.66** 85.02±4.05
HDL-C (mg/dl) 25.60±1.53 10.20±0.66 15.02±0.71* 20.26±0.66 23.82±0.57**
LDL-C (mg/dl) 31.12±1.75 120.68±5.68 61.66±3.75** 55.85±0.87 42.82±0.83**
VLDL-C (mg/dl) 14.88±1.18 35.32±1.37 20.52±1.37 18.36±1.13 17.00±0.81
Each value is mean±SEM of 6 rats in each group. *p<0.05, **p<0.01 and ***p<0.001 in comparison to diabetic control. EEAI: Ethanolic extract of Alocasia indica,
TG: Triglyceride, HDL-C: High-density lipoprotein cholesterol, LDL-C: Low-density lipoprotein cholesterol, VLDL-C: Very low-density lipoprotein cholesterol, High fat
diet, STZ: Streptozotocin, SEM: Standard error of mean
Srinivasan K, Viswanad B, Asrat L, Kaul CL, Ramarao P. Combination
of high-fat diet-fed and low-dose streptozotocin-treated rat: A model
for type 2 diabetes and pharmacological screening. Pharmacol Res
;52(4):313-20.
Larner J. The Pharmacological Basis of Therapeutics. 7
Standard
group
Standard
group
ed. New York:
Macmillan; 1985.
th
Bailey CJ, Day C. Traditional plant medicines as treatments for
diabetes. Diabetes Care 1989;12(8):553-64.
WHO Expert Committee on Diabetes Mellitus. Technical Reports
Series. Geneva: World Health Organization; 1980.
Gupta RK, Kesari AN, Murthy PS, Chandra R, Tandon V, Watal G.
Hypoglycemic and antidiabetic effect of ethanolic extract of leaves
of Annona squamosa L. in experimental animals. J Ethnopharmacol
;99(1):75-81.
Alarcon-Aguilara FJ, Roman-Ramos R, Perez-Gutierrez S, AguilarContreras
A,
Contreras-Weber
CC,
Flores-Saenz JL.
Study of
the antihyperglycemic
effect
of
plants used
as antidiabetics. J
Ethnopharmacol
;61(2):101-10.
Srinivasan K, Ramarao P. Animal models in type 2 diabetes research; an
overview. Indian J Med Res 2007;125(3):451-72.
Mulla WA, Salunkhe RV, Kuchekar VR. Free radical scavenging
activity of leaves of Alocasia indica. Indian J Pham Sci
;71(3):303-7.
Mulla WA, Salunkhe VR, Bhise SB. Hepatoprotective activity of
hydroalcoholic extract of leaves of Alocasia indica (Linn.). Indian J
Exp Biol 2009;47(10):816-21.
Mulla WA, Chopade AR, Bhise SB, Burade KB, Khanwelkar CC.
Evaluation of antidiarrheal and in vitro antiprotozoal activities of
extracts of leaves of Alocasia indica. Pharm Biol 2011;49(4):354-61.
Mulla WA, Vard ST, Rohan VP. Evaluation of anthelmintic activity of
leaves of Alocasia indica. Int J PharmTech Res 2010;2:26-30.
Mulla WA, Sargade PB, Parwar AM. Evaluation of antimicrobial activity
of leaves of Alocasia indica. Int J PharmTech Res 2010;2:327-33.
WHO. Quality Control Methods for Medicinal Plants Materials.
Geneva: World Health Organization; 1998. p. 32.
Trease GE, Evans WC. A Text Book of Pharmacognosy. Oxford: ELSB
Baillere Tindal; 1987. p. 1055.
Zhou J, Zhou S, Tang J, Zhang K, Guang L, Huang Y, et al. Protective
effect of berberine on beta cells in streptozotocin- and highcarbohydrate/high-fat
diet-induced
diabetic
rats.
Eur J Pharmacol
;606(1-3):262-8.
Kumar EK, Janardhana GR. Antidiabetic activity of alcoholic stem
extract of Nervilia plicata in streptozotocin-nicotinamide induced
type 2 diabetic rats. J Ethnopharmacol 2011;133(2):480-3.
Friedewald WT, Levy RI, Fredrickson DS. Estimation of the
concentration of low-density lipoprotein cholesterol in plasma, without
use of the preparative ultracentrifuge. Clin Chem 1972;18(6):499-502.
Asian J Pharm Clin Res, Vol 8, Issue 6, 2015, 58-62
Jawaid et al.
Beppu H, Shimpo K, Chihara T, Kaneko T, Tamai I, Yamaji S, et al.
Antidiabetic effects of dietary administration of Aloe arborescens Miller
components on multiple low-dose streptozotocin-induced diabetes in
mice: Investigation on hypoglycemic action and systemic absorption
dynamics of aloe components. J Ethnopharmacol 2006;103(3):468-77.
Davis SN, Granner DK. Insulin, oral hypoglycemic agents and the
pharmacology of endocrine pancreas. In: Hardman JG, Limberd LE,
Malinoff PB, Ruddon RW, Gilman AG, editors. Goodman and Gilman’s
the Pharmacological Basis of Therapeutics. 9
ed. New York:
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