THE CONTROL OF APRICOT SEED DORMANCY AND GERMINATION BY LOW TEMPERATURE TREATMENTS
DOI:
https://doi.org/10.22159/ijags.2021.v9i1.40689Keywords:
apricot (PrunusarmeniacaL), dormancy, stratification, germination, seedling growth, chemical constituentsAbstract
Objective: Freshly harvested seeds of “Local” apricot variety were found to be dormant and did not germinate at all. A specific low-temperature stratification treatment was required to overcome seed dormancy. The most effective temperature for breaking seed dormancy, germination, and seedling growth was 5°C cold stratification (CS). Increased seed germination percentage was recorded when the period of stratification prolonged. Seedling developed from stratified seeds had better growth than those developed from non-stratified seeds.
Methods: For stratification treatments, the seeds with removed endocarp were mixed with moistened sand. Afterward, they were subjected to a period of stratification at 5°C. Seeds were stratified in pots of 30 cm×40 cm. Stratified seeds were regularly irrigated once per week. To prevent the water loss during stratification upper surface of pots was covered by a sack. The following stratification was applied for apricot variety: CS for 0, 3, 6, 9, 12, and15 days in 1998 and 1999 years for “Local” variety.
Results: Apricot seeds required a CS of about 15 days for “Local” variety to reach maximum germination and normal seedling growth. Moreover, when stratification period was prolonged, some of the chemical constituents of apricot seeds were increased and other was decreased. Therefore, it can be suggested that breaking of dormancy is coincided with several changes in different chemical constituents of seeds. Some of these materials increased (total, reducing and non-reducing sugars, total free amino acids, total indoles, and total and conjugated phenols) and other materials such as free phenols which decreased at seed germinations.
Conclusion: The most effective temperature for breaking seed dormancy, germination, and seedling growth was 5°C CS. Increased seed germination percentage was recorded when the period of stratification prolonged. Seedling developed from stratified seeds had better growth than those developed from non-stratified seeds.
References
Şan B, Yildirim AN, Yildirim F. An in vitro germination technique for some stone fruit species: The embryo isolated from cotyledons successfully germinated without cold pre-treatment of seeds. Am Soc Hortic Sci 2014;49:294-6.
Mousavi SR, Rezaei M, Mousavi A. A general overview on seed dormancy and methods of breaking it. Adv Environ Biol 2011;5:3333-7.
García-Gusano M, Martínez-Gómez P, Dicenta F. Breaking seed dormancy in almond (Prunus dulcis (Mill.) D.A. Webb). Sci Hortic 2004;99:363-70.
Shah RA, Sharma VK, Jasrotia WA, Plathia M. Effect of seed priming on peach, plum and apricot germination and subsequent seedling growth. Indian J Hortic 2013;70:591-4.
Baskin JM, Baskin CC. A classification system for seed dormancy. Seed Sci Res 2004;14:1-16.
Finch-Savage WE, Leubner-Metzger G. Seed dormancy and the control of germination. New Phytol 2006;171:501-23.
El-Yazal MA, Rady MM, El-Yazal SA. Metabolic changes in polyamines, phenylethylamine, and arginine during bud break in apple flower buds under foliar-applied dormancy-breaking. Int J Empir Educ Res 2018c;1:1-18.
El-Yazal MA. Seasonal changes in soluble and non-soluble carbohydrates during and after dormancy release in early and late varieties of apple (Malus Sylvestris, Mill) trees. Int J Empir Educ Res 2019b;3:1-18.
El-Yazal MA. Impact of chilling requirement on budburst, floral development and hormonal level in buds of early and late apple varieties (Malus sylvestris, Mill) under natural conditions. J Hortic Plant Res 2019c;8:1-11.
Sel-Yazal MA. Impact of chilling requirements on metabolic changes in nitrogenous compounds in buds during and after dormancy releasing in early and late (Malus sylvestris, Mill) apple varieties. Int Lett Nat Sci 2021;81:13-22.
El-Yazal MA, El-Yazal SA. Impact of chilling requirements on metabolic changes in nitrogenous compounds in buds during and after dormancy releasing in early and late (Malus sylvestris, Mill) apple varieties. Hortic Int J 2019a;3:230-8.
Han M, Zhang M, Tian Y, Zhang W, Zhang J. Effect of plant hormones on seed dormancy and seedling growth of stone fruits. Acta Bot Boreali Occident Sin 2002;22:1348-54.
Martinez-Gomez P, Dicenta F. Mechanisms of dormancy in seeds of peach [Prunus persica (L.) Batsch] cv. GF305 .Sci Hortic 2001;91:51-8.
Arbeloa A, Daorden M, Garci E, Andreu P, Marin JA. In vitro culture of ‘Myrobalan’ (Prunus cerasifera Ehrh.) embryos. Hortic Sci 2009;44:1672-4.
Rady MM, El-Yazal MA. Response of “Anna” apple dormant buds and carbohydrate metabolism during floral bud break to onion extract. Sci Hortic 2013;155:78-84.
Rady MM, El-Yazal MA. Garlic extract as a novel strategy to hasten dormancy release in buds of “Anna” apple trees. S Afr J Bot 2014;92:105-11.
El-Yazal MA, Rady MM. Changes in nitrogen and polyamines during breaking bud dormancy in “Anna” apple trees with foliar application some compounds. Sci Hortic 2012a;136:75-80.
El-Yazal MA, Rady MM, Seif SA. Foliar-applied dormancy-breaking chemicals change the content of nitrogenous compounds in the buds of apple (Malus sylvestris Mill. cv. Anna) trees. J Hortic Sci Biotechnol 2012b;87:299-304.
El-Yazal MA, Rady MM. Foliar-applied DormexTM or thiourea-enhanced proline and biogenic amine contents and hastened breaking bud dormancy in ‘‘Ain Shemer’’ apple trees. Trees 2013;27:161-9.
El-Yazal MA, Rady MM. Exogenous onion extract hastens bud break, positively alters enzyme activity, hormone, amino acids and phenol contents, and improves fruit quality in “Anna” apple trees. Sci Hortic 2014a;169:154-60.
El-Yazal MA, El-Yazal SA, Rady MM. Exogenous dormancy-breaking substances positively change endogenous phytohormones and amino acids during dormancy release in “Anna” apple trees. Plant Growth Regul 2014b;72:211-20.
El-Yazal MA, Rady MM, El-Yazal SA. Foliar-applied mineral oil enhanced hormones and phenols content and hastened breaking bud dormancy in “Astrachan” apple trees. Int J Empir Educ Res 2018a;1:57-73.
El-Yazal MA, El-Yazal SA, Rady MM. Changes in promoter and inhibitor substances during dormancy release in apple buds under foliar-applied dormancy-breaking agents. Int J Empir Educ Res 2018b;1:1-20.
El-Yazal MA, Rady MM, El-Yazal SA, Morsi ME. Changes in metabolic processes during break dormancy in apple buds under foliar-applied garlic extract. Int J Empir Educ Res 2018d;1:36-58.
El-Yazal MA, El-Yazal SA, Morsi ME, Rady MM. Onion extract application effects on flowering behavior and yield, and a few chemical constituents of shoots throughout dormancy break in “Anna” apple trees. J Hortic Plant Res 2019b;7:1-15.
El-Yazal MA. Effect of timing of mineral oil spraying on budburst and metabolic changes in “Barkhar” apple trees under conditions of inadequate winter chilling in Egypt. Hortic Int J 2019a;3:67-75.
Mark R, Jo-Ann B, Paul W, Ercheng L, Masaji K. Maturity and temperature stratification affect the germination of Styrax japonicus seeds. J Hortic Sci Biotechnol 2015;79:645-51.
Keun SJ, Hee KJ, Kyung LA. Effect of warm and cold stratification, and ethanol treatment on germination of Corylopsis seeds. Hortic Sci 2016;43:84-91.
Pliszko A, Kostrakiewicz-Gierałt K. Resolving the naturalization strategy of Solidago × niederederi (Asteraceae) by the production of sexual ramets and seedlings. Plant Ecol 2017a;218:1243-53.
Pliszko A, Kostrakiewicz-Gierałt K. Seed germination in Solidago × niederederi (Asteraceae) and its parental species after two different fruit storage periods. Biodivers Res Conserv 2017b;48:19-24.
Pliszko A, Kostrakiewicz-Gierałt K. Effect of cold stratification on seed germination in Solidago × niederederi (Asteraceae) and its parental species. Biologia 2018;73:945-50.
Górnik K, Grzesik M, Janas R, Żurawicz E, Chojnowska E, Góralska R. The effect of apple seed stratification with growth regulators on breaking the dormancy of seeds, the growth of seedlings and chlorophyll fluorescence. J Hortic Res 2018;26:37-44.
Seng M, Cheong EJ. Comparative study of various pretreatment on seed germination of Dalbergia cochinchinensis. For Sci Technol 2020;16:68-74.
Yan A, Chen Z. The control of seed dormancy and germination by temperature, light and nitrate. Bot Rev 2020;86:39-75.
Guo C, Shen Y, Shi F. Effect of temperature, light, and storage time on the seed germination of Pinus bungeana Zucc. ex Endl.: The role of seed-covering layers and abscisic acid changes. Forests 2020;11:300.
Diaz DH, Martin GC. Peach seeds dormancy in relation to endogenous inhibitors and applied growth substances. J Am Soc Hortic Sci 1972;97:651-4.
AOAC. Official Methods of Analysis of the Association of Official Agricultural Chemists. 16th ed. Washington DC, USA: AOAC; 1995.
Jayaraman J. Laboratory Manual in Biochemistry. New York: Wiley Eastern Limited; 1981. p. 61-73.
Chen L, Chen Q, Zhang Z, Wana X. A novel colorimetric determination of free amino acids content in tea infusions with 2, 4-dinitrofluorobenzene. J Food Compost Anal 2009;22:137-41.
Larson P, Herlo A, Klunsour S, Asheim TA. On the biogenesis of some indole compounds in Acetobacter xylinum. Physiol Plant 1962;15:552-65.
Galicia L, Nurit E, Rosales A, Palacios-Rojas A. Laboratory Protocols: Maize Nutrition Quality and Plant Tissue Analysis Laboratory. Mexico: CIMMYT; 2009.
Duncan DB. Multiple range and multiple F tests. Biometrics 1955;11:1-42.
Eriş A. Bahçe Bitkileri Fizyolojisi. U Ü Z F Ders Notları 1990;11:152s.
Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 1962;15:473-97.
Serebryakova NV, Kalanova AI. The effect of water soluble vitamins on rose seed germination and rooting of cuttings. Hortic Abstr 1978;44:5819.
Tuzcu O, Kaplankıran M, Yesiloglu T, Ozcan M. The Effects of Germination and Developing of Different Preservation Methods on Pecan (Carya illinoensis) Seeds. Turkey First Nurseries Symposium. New Delhi: The Ministry of Agriculture Publication; 1991. p. 201-11.
Lewak S. Metabolic control of embryonic dormancy in apple seed: Seven decades of research. Acta Physiol Plant 2011;33:1-24.
Lewak S. Regulatory pathways in removal of apple seed dormancy. Acta Hortic 1981;120:149-59.
Bogatek R, Lewak S. Effect of cyanide and cold treatment on sugar catabolism in apple seeds during dormancy removal. Physiol Plant 1988;173:406-11.
Bewley JD, Black M. Seeds Physiology of Development and Germination. New York: Plenum Press; 1994. p. 445.
Kilany OA. Studies on germination of peach seeds. 1-Effect of seed coat, cold stratification and growth regulators. Ann Agric Sci 1986;24:2169-80.
Jones RL, Armstrong JE. Evidence for osmotic regulation of hydrolytic enzyme production in germinating barley seeds. Plant Physiol 1971;48:137-42.
Daskalyuk AP, Toma OK, Yarotskaya LV, Nikita II. Seed germination and polypeptide composition in early-and late-ripening apple cultivars as related to stratification time. Russ J Plant Physiol 1996;43:504-50.
Kopecky F, Sebanek J, Blazkova J. Time course of the changes in the level of endogenous growth regulators during the stratification of the seeds of the ‘‘Panenske ceske’’ apple. Biol Plant 1975;17:81-7.
Dziewanowska K, Lewak S. Non-decarboxylating transforma-tion of indol-3-acetic acid in apple seeds. Biol Plant 1987;29:110-7.
Dziewanowska K, Grochowska MJ, Lewak S. Changes inphloridzin and chlorogenic acid content and in indolylacetic acidoxidase activity during development of apple seeds. Fruit Sci Rep 1974;1:3-9.
Bogatek R, Podstolski A, Ostaszewska A, Lewak S. Phloridzin transformation and accumulation during the stratification of apple seeds and the culture of isolated embryos. Biol Plant 1976;18:241-50.
Come D. Relations entre l’oxygene et les phenomenes dedormance embryonnaire et d’inhibition tegumentaire. Bull Soc Frane Physiol Veg 1968;14:3145.
Dziewanowska K, Lewak S. Indolylacetic acid oxidase indormant apple embryos. Biol Plant 1975;17:207-13.
Kefeli V, Kutacek M. Phenolic substances and their possible role in plant growth regulation. Plant Growth Regul 1977;5:472.
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