GENETIC RELATEDNESS AMONG MAIZE INBRED LINES BASED ON ISSR MARKERS AND ITS ASSOCIATION WITH HETEROSIS AND HYBRID PERFORMANCE

AHMED MEDHAT MOHAMED AL-NAGGAR; AHMED MONGED SOLIMAN; MONA HASHEM HUSSIEN; SHAFIK DARWISH IBRAHIM; ELHAM MOHAMED HASSAN MOHAMED

doi:10.22159/ijags.2022.v10i6.46548

Authors

AHMED MEDHAT MOHAMED AL-NAGGAR Department of Agronomy, Faculty of Agriculture, Cairo University, Giza, Egypt.
AHMED MONGED SOLIMAN Department of Agronomy, Faculty of Agriculture, Cairo University, Giza, Egypt.
MONA HASHEM HUSSIEN Department of Genetics, Faculty of Agriculture, Cairo University, Giza, Egypt.
SHAFIK DARWISH IBRAHIM Department of Genome Mapping, Agricultural Genetic Engineering Research Institute, Agricultural Research Center, Giza, Egypt.
ELHAM MOHAMED HASSAN MOHAMED Department of Agronomy, Faculty of Agriculture, Omar Elmokhtar University, Al Bayda’, Libya.

DOI:

https://doi.org/10.22159/ijags.2022.v10i6.46548

Keywords:

Zea mays, ISSR, genetic diversity, heterosis

Abstract

The objectives of the present study were to measure the genetic diversity among eight inbred lines of maize using ISSR markers and the correlation coefficients between genetic diversity and each of heterosis and mean performance of hybrids for grain yield. Ten ISSR primers were used in the detection of polymorphism of the eight inbred lines in a laboratorial experiment. Heterosis and mean performance of grain yield/ha in their F1 diallel crosses were measured in a 2-year field experiment using a randomized complete blocks design with three replications. Based on ISSR markers, the genetic similarity coefficients among the eight maize inbred lines ranged from 0.798 (between L17 and IL53) to 0.943 (between IL80 and IL84) with an average of 0.869. Unique bands associated with maize inbred lines were identified. The results revealed that the genetic diversity among the inbred lines based on ISSR markers showed a significant, and negative relationship with mid-parent heterosis and mean performance of grain yield/ha. Further, intensive investigation of a large set of maize inbred lines from diverse populations using a large number of ISSR primers is required for proper understanding of genetic diversity of maize crop. Findings will be valuable for maize breeder, to practice effective selection of parental inbred lines for obtaining maximum heterosis and high mean grain yield/ha in their hybrids.

Author Biographies

AHMED MONGED SOLIMAN, Department of Agronomy, Faculty of Agriculture, Cairo University, Giza, Egypt.

Professor of plant breeding, Agronomy department, Faculty of Agriculture, Cairo university

MONA HASHEM HUSSIEN, Department of Genetics, Faculty of Agriculture, Cairo University, Giza, Egypt.

Professor, Genetics Department, Faculty of Agric., Cairo University, Egypt

SHAFIK DARWISH IBRAHIM, Department of Genome Mapping, Agricultural Genetic Engineering Research Institute, Agricultural Research Center, Giza, Egypt.

Department of Genome Mapping, Agricultural Genetic Engineering Research Institute, Agricultural Research Center, Giza, Egypt.

ELHAM MOHAMED HASSAN MOHAMED, Department of Agronomy, Faculty of Agriculture, Omar Elmokhtar University, Al Bayda’, Libya.

Department of Agronomy, Faculty of Agriculture, Omar Elmokhtar University, Libya

References

Adetimirin VO, Vroh-Bi I, The C, Menkir A, Mitchell SE, Kresovich S. Diversity analysis of elite maize inbred lines adapted to west and central Africa using SSR markers. Maydica 2008;53:143-9.

Al-Naggar AM, El-Salam RM, Badran AE, El-Moghazi MA. Molecular differentiation of five Quinoa (Chenopodium quinoa Willd.) genotypes using inter-simple sequence repeat (ISSR) markers. Biotechnol J Int 2017;20:1-12.

Al-Naggar AM, Shafik MM, Musa RY. Genetic diversity based on morphological traits of 19 maize genotypes using principal component analysis and GT biplot. Annu Res Rev Biol 2020;35:68-85.

Amorim EP, Amorim VB, dos Santos JB, de Souza AP, de Souza JC. Genetic distance based on SSR and grain yield of inter and intra-population maize single cross hybrids. Maydica 2006;51:507-13.

Balestre M, Von Pinho RG, Souza JC, Lima JL. Comparison of maize similarity and dissimilarity genetic coefficients based on microsatellite markers. Genet Mol Res 2008;7:695-705.

Berilli AP, Pereira MG, Gonçalves LS, da Cunha KS, Ramos HC, Filho GA, et al. Use of molecular markers in reciprocal recurrent selection of maize increases heterosis effects. Genet Mol Res 2011;10:2589-96.

Beyene Y, Botha AM, Myburg AA. A comparative study of molecular and morphological methods of describing genetic relationships in traditional Ethiopian highland maize. Afr J Biotechnol 2005;4:586-95.

Bhusal TN, Lal GM. Relationship among heterosis, combining ability and SSR based genetic distance in single cross hybrids of maize (Zea mays L). Vegetos 2017;30:17-26.

Devi P, Singh NK. Heterosis, molecular diversity, combining ability and their nterrelationships in short duration maize (Zea mays L) across the environments. Euphytica 2011;178:71-81.

Dhliwayo T, Pixley K, Menkir A, Warburton ML. Combining ability, genetic distances and heterosis among elite CIMMYT and IITA tropical maize inbred lines. Crop Sci 2009;49:1201-10.

Duvick DN. Heterosis: Feeding people and protecting natural resources. In: Coors JG, Pandey S, Editors. The Genetics and Exploitation of Heterosis in Crops. Madison, WI: American Society of America; 1999. p. 19-29.

Food and Agriculture Organization. FAOSTAT. Rome: Food and Agriculture Organization, Statistical Division; 2022. Available from: https://www. fao.org/faostat/en/#data/QC [Last accessed on 2022 Jun 24].

Fufa H, Baenziger PS, Beecher BS, Dweikat I, Graybosch RA, Eskridge KM. Comparison of phenotypic and molecular marker-based classifications of hard red winter wheat cultivars. Euphytica 2005;145:133-46.

George ML, Salazar F, Warbuton M, Narro L, Vallejo FA. Genetic distance and hybrid value in tropical maize under P stress and non-stress conditions in acid soils. Euphytica 2011;178:99-109.

Govindaraj M, Vetriventhan M, Srinivasan M. Importance of genetic diversity assessment in crop plants and its recent advances: An overview of its analytical perspectives. Genet Res Int 2015;2015:431-87.

Gupta PK, Varshney RK, Sharma PC, Ramech B. Molecular markers and their applications in wheat breeding. Plant Breed 1999;118:369-90.

Hallauer AR, Carena MJ, Filho JB. Handbook of Plant Breeding: Quantitative Genetics in Maize Breeding. New York, USA: Springer; 2010.

Hammer AT, David AT, Paul DR. PAST: Palaeontological statistics software package for education and data analysis. Palaeontol Electronica 2001;4:9.

Ibrahim SD, El-Hakim AF, Ali HE, El-Maksoud RM. Genetic differentiation using ISSR, SCoT and DNA barcoding for Quinoa genotypes. Arab J Biotech 2019;22:103-18.

Legesse BW, Myberg AA, Pixley KV, Twumasi-Afriyie S, Botha AM. Relationship between hybrid performance and AFLP based genetic distance in highland maize inbred lines. Euphytica 2008;162:313-23.

Lenka D, Tripathy SK, Kumar R, Behera M, Ranjan R. Assessment of genetic diversity in quality protein maize (QPM) inbreds using ISSR markers. J Environ Biol 2015;36:985-92.

Ndhlela T, Herselman L, Semagn K, Magorokosho C, Mutimaamba C, Labuschagne MT. Relationships between heterosis, genetic distances and specific combining ability among CIMMYT and Zimbabwe developed maize inbred lines under stress and optimal conditions. Euphytica 2015;204:635-47.

Pajic Z, Eric U, Drinic SM, Srdic J, Filipovic M. Genetic divergence estimated by RAPD markers and its relationship with hybrid performance in popcorn. Cereal Res Commun 2010;38:184-92.

Santos LF, Andueza-Noh RH, Ruíz ES, Latournerie-Moreno L, Garruña R, Mijangos-Cortes JO, et al. Characterization of the genetic structure and diversity of maize (Zea mays L) landrace populations from Mexico. Maydica Electron Publica 2017;62: 1-7.

Soliman ER, El-Shazly HH, Börner A, Badr A. Genetic diversity of a global collection of maize genetic resources in relation to their subspecies assignments, geographic origin, and drought tolerance. Breed Sci 2021;71:313-25.

Spooner DM, Tivang J, Nienhis J, Miller JT, Douches DS, Contreras-M A. Comparison of four molecular markers measuring relationship among the wild potato relatives Solanum section Etuberosum (subgenus Potato). Theor Appl Genet 1996;92:532-40.

Tanvir HD, Shakeel R, Verma S. Comparative germplasm characterization of maize (Zea mays L.) in Rajouri region of Pir Panjal Himalaya J and K (India), based on morphological and ISSR markers. J Crop Sci Biotech 2018;21:43-55.

Valdemar PC, Paulo MR, Claudete FR, Ferreira JM, Rosângela MM. Assessment of genetic diversity in maize (Zea mays L.) landraces using inter simple sequence repeat (ISSR) markers. Crop Breed Appl Biotechnol 2002;2:557-68.